satijalab / azimuth

A Shiny web app for mapping datasets using Seurat v4
https://satijalab.org/azimuth
GNU General Public License v3.0
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No feature overlap between existing object and new layer data #186

Open mainyanghr opened 11 months ago

mainyanghr commented 11 months ago

Please see the code, It seems my object does not have overlap with reference. How should I improve?

I am also confused with Normalization step, In the previous Vignette, it was suggested to use STC transormation.

names(object) [1] "RNA" "HTO" "ADT" "RNA_nn" "RNA_snn" "wknn" "wsnn" "pca"
[9] "umap" "harmony" "apca" "wnn.umap" "rna.umap" "adt.umap"

> names(object@assays) [1] "RNA" "HTO" "ADT"

**> objquery <- RunAzimuth(object,