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satijalab / sctransform

R package for modeling single cell UMI expression data using regularized negative binomial regression
GNU General Public License v3.0
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SCTransform Normalize Sequencing Depth #51

Open ShaowenJ opened 4 years ago

ShaowenJ commented 4 years ago

Hi, I just have a quick question, How does SCTransform Normalize Sequencing Depth across all cells, do you need to specify the argument vars.to.regress = "nCount_RNA" in the SCTransform function? Or it automatically would do that?

Thanks!

ShaowenJ commented 4 years ago

And one more question if you don't mind, What's the difference between "ScaleData(obj, vars.to.regress = "nCount_RNA")

Thanks!