Barcode file missing. Expecting barcodes.tsv.gz

[algebio]

Hi (not sure this is a bug, please correct if mistaken)

I'm running the multimodal_vignette.Rmd but I got stack in one of the last chunks of code to run. (# Loading data from 10X multi-modal experiments). I downloaded two featured filtered files with format h5 and tar.gz from here and I stored them in a folder with same path as the next line requires.

When I run the next line, I get this error:

pbmc10k.data <- Read10X(data.dir = '../data/pbmc10k/filtered_feature_bc_matrix/')

Error in Read10X(data.dir = "C:/Users/njo47/OneDrive - Newcastle University/Documents/Seurat/data/pbmc10k/") : Barcode file missing. Expecting barcodes.tsv.gz 2. stop("Barcode file missing. Expecting ", basename(path = barcode.loc)) 1. Read10X(data.dir = "C:/Users/njo47/OneDrive - Newcastle University/Documents/Seurat/data/pbmc10k/")

How do I get or download tsv.gz files?

Thank you for your help.

Regards Juan

[samuel-marsh]

Hi Juan,

Not member of dev team but hopefully can be helpful. If you have the H5 files you can just use the Read10X_h5 function instead of Read10X.

Best, Sam

[algebio]

Hi Sam

Great answer. Thank you for your help!

Regards Juan