How to specify multiple technologies as the query set

[ksaunders73]

Hello!

This may be a simple question, but what if, in the following code from the vignette, I wanted to not just make fluidigmc1the query, but also smartseq2?

pancreas.query <- pancreas.list[["fluidigmc1"]]

#output of pancreas.query will be input into the following lines of code:
pancreas.anchors <- FindTransferAnchors(reference = pancreas.integrated, query = pancreas.query,
    dims = 1:30, reference.reduction = "pca")
predictions <- TransferData(anchorset = pancreas.anchors, refdata = pancreas.integrated$celltype,
    dims = 1:30)
pancreas.query <- AddMetaData(pancreas.query, metadata = predictions)

Thanks for reading!

[yuhanH]

Hi, You may consider to do the above steps twice. It means you transfer the annotations for fluidigmc1 and smartseq2 separately. Then you can merge them in the end. Or you can use the below line.

pancreas.query <- pancreas.list[c("fluidigmc1", "smartseq2")]