Error in drawing dotplot

Hi, thank you very much for developing Seurat. This is my first time asking questions on the platform, please feel free to point out any mistakes.

As I was trying to create a dotplot for my Seurat object, I realised that they are not in the order of what I wanted, thus I reordered the active.ident as the following: hip@active.ident <- factor(hip@active.ident, levels = c("Granule Cells", "Interneuron", "CA1/CA3 Neuron", "Neuroblasts", "Astrocytes", "Radial Glia-like Cells", "MOL", "MFOL", "OPC", "COP", "Vascular Endothelial Cells", "Leptomeningeal Cells", "Ependymall Cells", "Macrophages/Microglia", "Undefined"))

By doing so, the levels in active.ident are in different order. Which allow me to bring the SeuratObject to the next step for DotPlot. { d <- hip DefaultAssay(d) <- "RNA" length(colnames(d)) markers.to.plot <- c("MOG", "MBP") DotPlot(d, features = markers.to.plot, cols = c("blue", "red"), dot.scale = 8) + RotatedAxis() }

However, here is where I face error as the following comes up: Warning: Found the following features in more than one assay, excluding the default. We will not include these in the final data frame: MOG, MBP Error in FetchData.Seurat(object = object, vars = features, cells = cells) : None of the requested variables were found: MOG, MBP In addition: Warning message: In cells.idents["NA"] <- names(x = which(x = is.na(x = Idents(object = object)[cells]))) : Error in FetchData.Seurat(object = object, vars = features, cells = cells) : None of the requested variables were found: MOG, MBP

I am not entirely sure what these error messages indicates, as before I alter the active.ident order the DotPlot can be illustrated very easily without any issue.

Thank you very much.

Seurat (4.1.1) R version 4.1.2 (2021-11-01) Platform: x86_64-apple-darwin20.6.0 (64-bit) Running under: macOS Monterey 12.3.1

satijalab / seurat

Error in drawing dotplot #6027