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satijalab / seurat

R toolkit for single cell genomics
http://www.satijalab.org/seurat
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Can I analyze flow cytometry data with Seurat ? #6492

Closed Nidane closed 2 years ago

Nidane commented 2 years ago

Hi team, thanks a lot for such a useful tool!

I wonder other than conventional scRNA-seq data, whether Seurat could be used to analyze other single cell data like flow cytometry ones ?

i.e. if I convert flow cytometry results (.fcs files) into a dataframe-ish file (.csv etc., which is also like cells x genes/markers), whether I could convert such a dataframe into a Seurat object and analyze it with Seurat ?

Thanks.

yuhanH commented 2 years ago

HI Nidane Sure. When you load the matrix into seurat, you can analyze it in the same way that you would ADT data. https://satijalab.org/seurat/articles/multimodal_vignette.html