satijalab / seurat

R toolkit for single cell genomics
http://www.satijalab.org/seurat
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Number of cell in SCTramsform() #9505

Open Chrisdoan9 opened 5 hours ago

Chrisdoan9 commented 5 hours ago

Hi team,

I use a reference data set with around 160,000 cell, so may I know how many cells should I use in this case? Is 40,000 cells good enough? Thank you so much!

allen_reference <- SCTransform(allen_reference, ncells = 40000, verbose = FALSE) %>%
  RunPCA(verbose = FALSE) %>%
  RunUMAP(dims = 1:30)