Open dcopetti opened 2 years ago
Hello, just checking on on this: do you have any comment on my results above? THanks
Hi @dcopetti ,
I just saw your message. I hope you had found the way to process your data.
If not yet, let me know.
Best, Hequan
Hello, I am looking for some advice on how to run findGSE and how to interpret the data when dealing with heterozygosity and plant genomes. I summarized the runs on 4 species in this file: FindGSE_tests_220124.pdf The results show inaccuracy in genome size estimation as well as inconsistency in the resulting values when parameters change.
Briefly:
exp_hom=NN
or not - all 4 casesexp_hom=NN
at the mode of the homo peak, no estimation results (except for Cgil - because the het peak is buried?exp_hom=NN
larger than the mode of the homo peak, the estimation does not changeexp_hom=NN
values, some estimations vary by a lot, some by very little.by species:
exp_hom=NN
LOWER than the homo peakThe documentation says that the
exp_hom=NN
should be between the homo peak and its double2*hom_peak>x>hom_peak !
and I see that there is consistency in the estimations in that. The only thing is, sometimes the values are correct (Caus), others they are off (Cgig, Lmul). Can you please detail some guidelines on how to use the tool and get a reliable and consistent estimation in the case that the flow cytometry value is not known? Thanks!