leticiaquino
commented
8 years ago @gustavofonseca e @asael-silva
- [x] elemento-article-categories.rst
...
<article-categories>
<subj-group subj-group-type="heading">
<subject>Artigo Original</subject>
</subj-group>
</article-categories>
...- [x] elemento-award-group.rst
<award-group>
<funding-source>Fundação de Amparo à Pesquisa do Estado de São Paulo</funding-source>
<award-id>2013/24633-2</award-id>
</award-group>- [x] elemento-back.rst
<back>
<ref-list>
<title>References</title>
<ref id="B1">
<mixed-citation>Associação Brasileira das Indústrias Exportadoras de Carnes. (2012, fevereiro 29). [Entrevista com o Antônio Camardelli]. Presidente da ABIEC. Retrieved from http://www.abiec.com.br/noticia.asp?id=779#.Uj8VnWt5mSM </mixed-citation>
<element-citation publication-type="book">
<person-group person-group-type="author">
<collab>Associação Brasileira das Indústrias Exportadoras de Carnes</collab>
</person-group>
<year>2012,</year>
<source>[Entrevista com o Antônio Camardelli]. Presidente da ABIEC</source>
<ext-link ext-link-type="uri" xlink:href="http://www.abiec.com.br/noticia.asp?id=779#.Uj8VnWt5mSM">http://www.abiec.com.br/noticia.asp?id=779#.Uj8VnWt5mSM</ext-link>
</element-citation>
</ref>
<ref id="B2">
<mixed-citation>Blumentritt, T. P. (2003). Foreign subsidiaries' government affairs activities. Business & Society, 42(2), 202-233. doi: 10.1177/0007650303042002003</mixed-citation>
<element-citation publication-type="journal">
<person-group person-group-type="author">
<name>
<surname>Blumentritt</surname>
<given-names>T. P.</given-names>
</name>
</person-group>
<year>2003</year>
<article-title>Foreign subsidiaries' government affairs activities</article-title>
<source>Business & Society</source>
<volume>42</volume>
<issue>2</issue>
<fpage>202</fpage>
<lpage>233</lpage>
<pub-id pub-id-type="doi">10.1177/0007650303042002003</pub-id>
</element-citation>
</ref>
...
</ref-list>
<app-group>
<app id="app1">
<label>ANNEX A</label>
<table-wrap id="t6">
<label>Table 6</label>
<caption>
<title>Multivariate analysis of risk factors associated with readmission - Model 2</title>
</caption>
<graphic xlink:href="1234-5678-rctb-45-05-0110-gt031.tif"/>
</table-wrap>
</app>
</app-group>
</back>- [x] elemento-body.rst
<body>
<sec sec-type="intro">
<title>INTRODUCTION</title>
<p>Cyclin-dependent kinase-like 5 (CDKL5), which is also known as serine/threonine kinase 9 (STK9), is a protein kinase that is widely distributed in all tissues and highly expressed in the brain (<xref ref-type="bibr" rid="B10">Lin et al. 2005</xref>). CDKL5 is homologous to mitogen-activated protein kinases (MAPKs) and cyclin-dependent kinases (CDKs). Mutations in the gene that encodes CDKL5 cause intellectual disability, infantile spasms, and variant form of Rett syndrome, which is a neurodevelopmental disorder that is caused primarily by mutations in the methyl CpG binding protein 2 gene (<italic>MECP2</italic>) (<xref ref-type="bibr" rid="B5">Evans et al. 2005</xref>; <xref ref-type="bibr" rid="B7">Kalscheuer et al. 2003</xref>; <xref ref-type="bibr" rid="B11">Mari et al. 2005</xref>; <xref ref-type="bibr" rid="B17">Tao et al. 2004</xref>; <xref ref-type="bibr" rid="B18">Weaving et al. 2004</xref>). Because mutations in <italic>CDKL5</italic> and <italic>MECP2</italic> can cause similar phenotypes in patients, it is possible that CDKL5 and MeCP2 share the same molecular pathway in the central nervous system. Investigating the relationship of CDKL5 with MeCP2 and other interactors will help to further elucidate the critical roles of CDKL5 and MeCP2 in neural development, plasticity and neurological disorders. </p>
</sec>
<sec sec-type="materials|methods">
<title>MATERIALS AND METHODS</title>
<sec>
<title>Rat embryonic cortical neuron cultures</title>
<p>This study was approved by the Experimental Animal Ethics Committee of Peking University First Hospital (protocol number J201223). Primary cortical neurons were prepared from the brains of embryonic day 18 (E18) Sprague Dawley (SD) rats. Cell culture was performed as described previously (<xref ref-type="bibr" rid="B19">Zhang et al. 2006</xref>), with the following modifications. Cortical tissue from fetal rats was carefully dissected and digested with 0.25%Trypsin (Gibco) at 37℃ under 5%CO<sub>2</sub> for 5-8 min in 3.5 cm dishes. The digestion was terminated by the addition of 6-8 ml of Dulbecco Modified Eagle Medium (DMEM) (Gibco) supplemented with 10% FBS (Gibco). Then, the tissue was scattered with pipettes. The separated neurons were plated on poly-L-lysine-coated (Sigma) dishes and maintained first in DMEM with 10% FBS for 2-4 hours and then in Neurobasal<sup>(r)</sup> Medium (Gibco) supplemented with 2% B-27 Supplement (Gibco) and 1% L-Glutamine (200 mM, Gibco). Every other day, 50% of the medium volume was replaced.</p>
</sec>
<sec>
<title>Antibodies </title>
<p>The following primary antibodies were used in this study: rabbit polyclonal anti-CDKL5 (Abcam, ab191510), rabbit polyclonal anti-CDKL5 (Santa Cruz Biotechnology), mouse monoclonal anti-MeCP2 (Abcam, ab50005), mouse monoclonal anti-MAP2 (Abcam), rabbit monoclonal anti-Dnmt1 (Cell Signaling Technology), and rabbit monoclonal anti-β-Actin (Cell Signaling Technology). The secondary antibodies used were the HRP Goat anti-Mouse IgG Antibody (Abgent) and the HRP Goat anti-Rabbit IgG Antibody (Abgent). </p>
</sec>
</sec>
<sec sec-type="results">
<title>RESULTS</title>
<p>To elucidate whether CDKL5 can bind to MeCP2 and Dnmt1 during rat neuronal in vitro differentiation, co-immunoprecipitation was performed. Incubation of polyclonal anti-CDKL5 with primary neuronal cell lysate samples at DIV4 and subsequent western blot analysis of MeCP2 and Dnmt1 revealed that an interaction occurs among endogenous CDKL5, MeCP2 and Dnmt1.</p>
</sec>
</sec>
</body>- [x] elemento-collab.rst
Em Front:
<contrib-group>
<contrib contrib-type="author">
<collab>BFG - The Brazil Flora Group</collab>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Zappi</surname>
<given-names>Daniela C.</given-names>
</name>
<xref ref-type="corresp" rid="c1">1</xref>
</contrib>
</contrib-group>Em back:
<element-citation publication-type="book">
<person-group person-group-type="author">
<collab>World Health Organization (WHO)</collab>
</person-group>
<source>The top 10 causes of death. Fact sheet nº 310</source>
<date-in-citation content-type="updated">Updated May 2014</date-in-citation>
<date-in-citation content-type="access-date">citado 2012 Out 10</date-in-citation>
<comment>Disponível em:. <ext-link ext-link-type="uri" xlink:href="http://www.who.int/mediacentre/factsheets/fs310/en/index2.html">http://www.who.int/mediacentre/factsheets/fs310/en/index2.html</ext-link>
</comment>
</element-citation>- [x] elemento-front.rst
<front>
<journal-meta>
<journal-id journal-id-type="publisher-id">bjos</journal-id>
<journal-title-group>
<journal-title>Brazilian Journal of Oral Sciences</journal-title>
<abbrev-journal-title abbrev-type="publisher">Braz. J. Oral Sci.</abbrev-journal-title>
</journal-title-group>
<issn pub-type="ppub">1677-3217</issn>
<issn pub-type="epub">1677-3225</issn>
<publisher>
<publisher-name>Faculdade de Odontologia de Piracicaba - UNICAMP</publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id pub-id-type="doi">10.1590/1677-3225v14n4a01</article-id>
<article-categories>
<subj-group subj-group-type="heading">
<subject>Original Articles</subject>
</subj-group>
</article-categories>
<title-group>
<article-title>Dentists' actions about oral health of individuals with Down Syndrome</article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname>Lira</surname>
<given-names>Ana de Lourdes Sá de</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Silva</surname>
<given-names>Claudio Inácio Reis da</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Rebelo</surname>
<given-names>Sylvana Thereza de Castro Pires</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>1</sup>
</xref>
</contrib>
<aff id="aff1">
<label>1</label>
<institution content-type="original">Universidade Estadual do Piauí - UESPI, School of Dentistry, Department of Clinical Dentistry, Area of Integrated Clinic, Parnaíba, PI, Brazil</institution>
<institution content-type="normalized">Universidade Estadual do Piauí</institution>
<institution content-type="orgname">Universidade Estadual do Piauí</institution>
<institution content-type="orgdiv1">School of Dentistry</institution>
<institution content-type="orgdiv2">Department of Clinical Dentistry</institution>
<addr-line>
<named-content content-type="city">Parnaíba</named-content>
<named-content content-type="state">PI</named-content>
</addr-line>
<country country="BR">Brazil</country>
</aff>
</contrib-group>
<author-notes>
<corresp id="c1">Correspondence to: Ana de Lourdes Sá de Lira Universidade Estadual do Piauí, Faculdade de Odontologia Rua Senador Joaquim Pires 2076 - Ininga CEP: 64049-590 Teresina, PI, Brasil Phone: +55 86 xxxxxxxxx E-mail: <email>foo@foobar.com</email>
</corresp>
</author-notes>
<pub-date pub-type="epub-ppub">
<season>Oct-Dec</season>
<year>2015</year>
</pub-date>
<volume>14</volume>
<issue>04</issue>
<fpage>256</fpage>
<lpage>261</lpage>
<history>
<date date-type="received">
<day>21</day>
<month>10</month>
<year>2015</year>
</date>
<date date-type="accepted">
<day>09</day>
<month>12</month>
<year>2015</year>
</date>
</history>
<permissions>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/4.0/" xml:lang="en">
<license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License</license-p>
</license>
</permissions>
<abstract>
<title>Abstract</title>
<sec>
<title>Aim:</title>
<p> To investigate the knowledge and actions of dentists for treatment of individuals with Down syndrome. </p>
</sec>
<sec>
<title>Methods:</title>
<p> A questionnaire was applied to all the dentists (n=90) working at the FHS (Family Health Strategy) modules in the urban limits of Parnaíba, PI, Brazil. Four of the questions in the questionnaire were written according to the Theory of Planned Behavior Table and Likert scale (questions 6,7,9 and 15), in order to analyze the professionals' intentions. Sixteen objective questions were elaborated with the purpose of collecting information about the degree of the dentists' knowledge as regards the intention of attending courses in the patients with special needs area including DS, and interaction with other professionals and families. The option was to use a questionnaire applied to the dentists of the region, from August to November 2014. </p>
</sec>
<sec>
<title>Results:</title>
<p> It was found that most professionals were women and they considered themselves able to identify these patients. Among the professionals, 70% showed they had no difficulty in identifying the patient with DS, and 5.2% had no opinion about the subject. Only 6.6% of the professionals showed to be certain about their aptitude to attend to these patients; 70% were partially apt, that is, they were not absolutely sure about their aptness. There was a statistical relationship between the variables understanding and difficulty in the treatment. There was no statistical relationship between the variable capacity to identify, understanding of the needs and fitness variable in attendance. </p>
</sec>
<sec>
<title>Conclusions:</title>
<p> Patients with Down syndrome need more attention and care of dentists, they must also be involved in a multidisciplinary approach. Most of the professionals do not follow the procedures laid down by the Ministry of Health, but showed interest in attending a course in this area and there is a low number of SD patients being cared in Parnaíba, PI.</p>
</sec>
</abstract>
<kwd-group xml:lang="en">
<title>Keywords:</title>
<kwd>Down Syndrome</kwd>
<kwd>oral health</kwd>
<kwd>quality of life</kwd>
</kwd-group>
<counts>
<fig-count count="3"/>
<table-count count="0"/>
<equation-count count="0"/>
<ref-count count="22"/>
<page-count count="6"/>
</counts>
</article-meta>
</front>
asael-silva
gustavofonseca
Os arquivos dos elementos ainda pendentes de exemplo são: tagset/