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scutbioinformatic / causalcall

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Raw data is not stored in Raw/Reads/Read_[read#] so new segments cannot be identified. #2

Open N-damo opened 3 years ago

N-damo commented 3 years ago

Dear developer: could you give me some help? command:python ../raw.py -i ~/Nanopore_RNA_directly_Sequencing/data/RawData/20200902RNA/20200902_1234_MN27328_FAL79918_bc368336/fast5_pass/ -o train error: Raw data is not stored in Raw/Reads/Read_[read#] so new segments cannot be identified. best regards LianLin

demizeng commented 3 years ago

Dear LianLin: As the error message shows, the raw signal in your .fast5 file is not stored in the folder Raw/Reads/Read_[read#] . The raw.py only supports .fast5 file stored as below. --xxxx.fast5 　--Raw 　　--Reads 　　　--Read_xx 　　　　--Signal 　--UniqueGlobalKey

N-damo commented 3 years ago

Dear developer: I used Tombo V1.5.1 resquiggle signal, now the format is correct. Should I use tombo to train a resquiggle model to predict events for more precisely? best regards LianLin

demizeng commented 3 years ago

I am not sure. You can try it.