Dear developer,
Thanks for developing such a useful tools for spatial analysis. I have a question. Let's say I have 100 images, each with 20-30 markers, and I've constructed the adata. However, when I execute UMAP, it doesn't delineate any distinct clusters. Instead, all the cells are grouped together. Do you have any advice on addressing this issue? I performed the images one-by-one to get the cell type and integrated them together? Thanks for your kind help in advance!
Dear developer, Thanks for developing such a useful tools for spatial analysis. I have a question. Let's say I have 100 images, each with 20-30 markers, and I've constructed the adata. However, when I execute UMAP, it doesn't delineate any distinct clusters. Instead, all the cells are grouped together. Do you have any advice on addressing this issue? I performed the images one-by-one to get the cell type and integrated them together? Thanks for your kind help in advance!