Closed alanaleah89 closed 3 years ago
Hi,
zUMIs will paste the three bits of barcode into one unique string when extracting from your fastq reads, so in your whitelist you should give the expected combinations in a single file. You can use expand.grid()
in R to find the unique combinations easily.
There is no option inbuilt for several whitelists.
Best, C
Feel free to reopen if you need further input.
Is there a way to insert 3 separate whitelist files?
The BD Rapsody single cell analysis system contains 3 BCs. Can zUMIs accept this type of parameter and if so should this file path be separated by a comma or a space?