expected_barcodes file with dual indices

[carmensandoval]

How should one format the list of expected barcodes when using dual indices?

I am using zUMIs to process libraries generated with Takara mRNA LP with UMIs kit, and each library has a unique dual index.

Should my file be:

barcode_1_i5   barcode_1_i7
barcode_2_i5   barcode_2_i7
barcode_3_i5   barcode_3_i7
.
.
.

Thanks!

[cziegenhain]

Hi Carmen,

Your file should contain the concatenated barcode string of every expected combination: https://github.com/sdparekh/zUMIs/wiki/Barcodes#barcode-annotation

Best, Christoph

[carmensandoval]

Thanks!