Thank you for your awesome software! I got several errors in processing my smartseq3 data, and I have scanned some related_issues, yet it's not solved my problem. First, because several cells have the same index, I merged demultiplexed fastq files by script 'merge_demultiplexed_fastq.R'. Then I run zumis.sh by slurm_script, below are yaml file and error log (surprising, its size is 2.5GB),
smartseq3.yamlerror_log
The run would produce results files, but the 'feature.pdf' showed there was too much unused barcodes. I think this is wrong. I refered to the issuse310, but it wouldnt produce result if I didnt offer index_fastq files. Can you give me some kindful help to solve it? I will be deeply grateful. Thanks! @cziegenhain
Thank you for your awesome software! I got several errors in processing my smartseq3 data, and I have scanned some related_issues, yet it's not solved my problem. First, because several cells have the same index, I merged demultiplexed fastq files by script 'merge_demultiplexed_fastq.R'. Then I run zumis.sh by slurm_script, below are yaml file and error log (surprising, its size is 2.5GB), smartseq3.yaml error_log
The run would produce results files, but the 'feature.pdf' showed there was too much unused barcodes. I think this is wrong. I refered to the issuse310, but it wouldnt produce result if I didnt offer index_fastq files. Can you give me some kindful help to solve it? I will be deeply grateful. Thanks! @cziegenhain
