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Ray -- Parallel genome assemblies for parallel DNA sequencing
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Insert peaks (again but in 2.0.0-rc4) #49

Closed mscook closed 12 years ago

mscook commented 12 years ago

Hi Seb,

I'm running - Ray version: 2.0.0-rc4

We still get artificially detected "large peaks".

I've included the metrics:

General

['kmer', '17', '19', '21', '23', '25', '27', '29', '31', '33', '35'] ['c > 100 bp', '143', '147', '145', '142', '137', '124', '128', '132', '134', '126'] ['c > 100 bp total len', '5241893', '5465571', '5354470', '5255806', '5242270', '5257263', '5385963', '5405457', '5327053', '5406021'] ['c > 100 bp av len', '36656', '37180', '36927', '37012', '38264', '42397', '42077', '40950', '39754', '42904'] ['c > 100 bp N50', '124514', '139408', '161895', '161895', '162956', '159457', '164333', '159457', '159457', '159457'] ['c > 100 bp largest', '345228', '345245', '348268', '348294', '349160', '345344', '345370', '345526', '345526', '345439'] ['c > 500 bp', '121', '119', '113', '109', '112', '112', '118', '118', '115', '114'], ['c > 500 bp total len', '5238060', '5460711', '5348486', '5249896', '5237169', '5255489', '5384460', '5402805', '5323604', '5404203'] ['c > 500 bp average len', '43289', '45888', '47331', '48164', '46760', '46924', '45631', '45786', '46292', '47405'] ['c > 500 bp N50', '124514', '139408', '161895', '161895', '162956', '159457', '164333', '159457', '159457', '159457'] ['c > 500 bp largest', '345228', '345245', '348268', '348294', '349160', '345344', '345370', '345526', '345526', '345439'] ['s > 100', '125', '132', '129', '121', '125', '110', '115', '116', '117', '108'], ['s > 100 bp total len', '5245763', '5467786', '5356812', '5260052', '5244192', '5307461', '5432292', '5470661', '5389713', '5478137'] ['s > 100 bp av len', '41966', '41422', '41525', '43471', '41953', '48249', '47237', '47160', '46065', '50723'] ['s > 100 bp N50', '159462', '161919', '171861', '164333', '190540', '186846', '171660', '172749', '171272', '187079'] ['s > 100 bp largest', '345228', '345245', '535229', '535191', '535193', '345724', '345370', '345526', '345526', '345439'] ['s > 500 bp', '103', '104', '97', '88', '100', '98', '105', '102', '98', '96'] ['s > 500 bp total len', '5241930', '5462926', '5350828', '5254142', '5239091', '5305687', '5430789', '5468009', '5386264', '5476319'] ['s > 500 bp av', '50892', '52528', '55163', '59706', '52390', '54139', '51721', '53607', '54961', '57044'] ['s > 500 bp N50', '159462', '161919', '171861', '164333', '190540', '186846', '171660', '172749', '171272', '187079'] ['s > 500 bp largest', '345228', '345245', '535229', '535191', '535193', '345724', '345370', '345526', '345526', '345439']

Scaffolding Stats

['NumberOfNChars', 3870, 2215, 2342, 4246, 1922, 50198, 46329, 65204, 62660, 72116] ['NumberOfNCharRuns', 18, 15, 16, 21, 12, 14, 13, 16, 17, 18] ['MinNChars', 29, 23, 37, 56, 17, 881, 506, 3594, 83, 3474] ['MaxNChars', 390, 312, 252, 363, 357, 4357, 4334, 4458, 4379, 4384] ['AvNChars', 215.0, 147.66666666666666, 146.375, 202.1904761904762, 160.16666666666666, 3585.5714285714284, 3563.769230769231, 4075.25, 3685.8823529411766, 4006.4444444444443]]

Insert Stats (: sepearates multiple)

['meanIns', '344', '350', '351', '351', '351', '351:4422', '351:4426', '351:4426', '351:4424', '351:4423'], ['sdIns', '56', '50', '49', '49', '49', '49:24', '49:20', '49:20', '49:22', '49:24']

I've included the LibraryStatistics and Library0 files for kmer 35:

LibraryNumber: 0 InputFormat: Interleaved,Paired DetectionType: Automatic File: XXXX_proc.fastq NumberOfSequences: 8205596 Distribution: 35/Library0.txt Peak 0 AverageOuterDistance: 351 StandardDeviation: 49 Peak 1 AverageOuterDistance: 4423 StandardDeviation: 24

47 1 54 1 57 1 59 1 65 1 66 1 68 1 69 2 71 3 72 2 74 7 75 5 76 5 77 9 78 3 79 4 80 2 81 5 82 1 83 1 84 4 85 4 86 2 87 6 88 1 89 3 90 3 91 2 92 4 93 3 94 3 95 6 96 3 97 7 98 6 99 4 100 7 101 121 102 113 103 93 104 95 105 112 106 128 107 133 108 104 109 127 110 145 111 178 112 180 113 187 114 193 115 201 116 215 117 208 118 265 119 257 120 273 121 263 122 296 123 344 124 345 125 362 126 375 127 342 128 397 129 446 130 482 131 494 132 513 133 515 134 581 135 609 136 661 137 683 138 663 139 733 140 762 141 806 142 821 143 891 144 961 145 959 146 1046 147 1027 148 1114 149 1075 150 1189 151 1188 152 1225 153 1269 154 1340 155 1423 156 1403 157 1454 158 1505 159 1521 160 1562 161 1598 162 1620 163 1719 164 1710 165 1754 166 1757 167 2012 168 1931 169 1912 170 1982 171 1961 172 2029 173 2075 174 2119 175 2110 176 2147 177 2184 178 2158 179 2248 180 2222 181 2314 182 2346 183 2351 184 2361 185 2318 186 2362 187 2352 188 2379 189 2508 190 2416 191 2508 192 2536 193 2425 194 2375 195 2483 196 2544 197 2413 198 2402 199 2469 200 2509 201 2435 202 2573 203 2428 204 2433 205 2496 206 2510 207 2493 208 2578 209 2531 210 2652 211 2472 212 2421 213 2488 214 2457 215 2382 216 2553 217 2475 218 2456 219 2440 220 2431 221 2343 222 2500 223 2450 224 2377 225 2307 226 2384 227 2463 228 2330 229 2389 230 2362 231 2399 232 2349 233 2388 234 2353 235 2386 236 2284 237 2325 238 2374 239 2287 240 2347 241 2366 242 2269 243 2262 244 2261 245 2242 246 2220 247 2348 248 2197 249 2284 250 2332 251 2214 252 2268 253 2243 254 2143 255 2214 256 2226 257 2234 258 2142 259 2118 260 2192 261 2168 262 2152 263 2126 264 2229 265 2128 266 2162 267 2158 268 2151 269 2136 270 2156 271 2201 272 2200 273 2264 274 2250 275 2223 276 2263 277 2135 278 2231 279 2183 280 2336 281 2233 282 2268 283 2252 284 2308 285 2267 286 2329 287 2340 288 2293 289 2404 290 2272 291 2288 292 2469 293 2391 294 2441 295 2534 296 2448 297 2516 298 2529 299 2425 300 2612 301 2662 302 2732 303 2782 304 2876 305 2952 306 3094 307 3176 308 3301 309 3518 310 3664 311 3986 312 4174 313 4420 314 4805 315 5167 316 5657 317 6207 318 6835 319 7489 320 8414 321 9325 322 10344 323 11486 324 12684 325 14228 326 15928 327 17390 328 19347 329 20962 330 22709 331 24658 332 26985 333 28822 334 31205 335 32772 336 34470 337 36416 338 37924 339 39824 340 40501 341 41865 342 43719 343 44970 344 45913 345 46423 346 47696 347 48126 348 48847 349 49953 350 50303 351 50775 352 51165 353 51567 354 51607 355 52142 356 52288 357 52254 358 52345 359 52346 360 52078 361 51349 362 51441 363 51071 364 50682 365 50937 366 50411 367 50102 368 49922 369 49540 370 48842 371 48466 372 48273 373 47883 374 47479 375 46592 376 46501 377 45421 378 44737 379 44276 380 43646 381 43146 382 41802 383 41218 384 40094 385 39390 386 38392 387 37312 388 36124 389 34988 390 33370 391 32068 392 30878 393 29090 394 27928 395 26226 396 25191 397 23977 398 22608 399 21323 400 20070 401 18912 402 17747 403 16524 404 15490 405 14380 406 13500 407 12759 408 11992 409 11160 410 10352 411 9636 412 9086 413 8313 414 7739 415 7460 416 6908 417 6255 418 5861 419 5610 420 5171 421 4593 422 4294 423 4030 424 3728 425 3496 426 3164 427 2873 428 2653 429 2404 430 2215 431 2005 432 1861 433 1640 434 1485 435 1305 436 1250 437 1062 438 943 439 819 440 745 441 674 442 567 443 515 444 460 445 380 446 336 447 319 448 276 449 207 450 197 451 161 452 138 453 122 454 104 455 100 456 79 457 68 458 52 459 46 460 33 461 31 462 29 463 10 464 19 465 23 466 11 467 14 468 10 469 9 470 12 471 10 472 2 473 2 474 4 475 3 476 4 477 4 478 2 479 1 480 2 481 2 482 3 483 1 484 1 485 2 486 2 488 1 489 1 490 4 493 2 494 1 495 1 497 1 499 3 505 2 506 2 507 2 508 1 511 3 513 1 517 1 520 2 523 1 525 1 528 1 529 1 535 1 537 4 538 1 540 1 544 2 545 3 546 1 549 1 551 1 552 1 554 1 555 1 559 1 561 1 567 1 571 1 572 1 586 1 587 3 592 1 594 1 598 1 604 1 607 1 612 1 621 1 630 1 632 1 636 1 639 1 649 1 655 1 656 1 657 1 670 1 679 1 682 1 683 1 684 1 690 1 693 1 695 2 708 1 713 1 716 1 721 1 732 2 733 1 755 1 779 1 780 1 808 1 895 1 973 1 1302 1 1497 1 2056 1 2237 1 3744 1 3937 1 3981 1 4279 2 4345 1 4350 1 4352 1 4353 1 4355 2 4358 1 4359 1 4361 1 4362 1 4363 2 4365 2 4368 1 4369 2 4370 3 4371 4 4372 2 4373 1 4375 2 4377 1 4379 1 4380 2 4382 2 4384 2 4385 1 4386 1 4387 4 4388 3 4389 2 4390 2 4391 3 4392 4 4393 5 4395 1 4396 1 4397 8 4398 6 4399 2 4401 3 4402 3 4403 5 4404 3 4405 6 4406 6 4407 4 4408 7 4409 4 4410 5 4411 5 4412 5 4413 6 4414 3 4415 4 4416 4 4417 12 4418 3 4419 5 4420 6 4421 6 4422 9 4423 7 4424 6 4425 5 4426 8 4427 8 4428 4 4429 2 4430 2 4431 5 4432 4 4433 5 4434 7 4435 4 4436 7 4437 6 4438 3 4439 3 4440 6 4441 6 4442 5 4443 4 4444 2 4445 6 4446 4 4447 4 4448 4 4449 6 4450 5 4451 4 4452 3 4453 3 4454 7 4455 6 4456 1 4457 2 4458 3 4459 3 4460 3 4461 1 4462 2 4463 2 4464 2 4466 1 4467 1 4468 2 4470 1 4471 1 4474 2 4479 1 4480 1 4484 1 4492 1 4494 1 4496 1 4501 1 4510 1 4511 1 4521 1 4532 1 4536 2 4543 1 4556 1 4561 1 4568 1 4581 1 4583 1 4585 1 4587 1 4597 1 4602 1 4616 1 4637 1 4721 1 4871 1 5126 1 5276 1 5848 1 6751 1 6868 1 7544 1 7996 1 8213 1 8528 1 13467 1 14233 1 16717 1 19963 1 20008 1 32159 1 34585 1 39611 1 40416 1 45612 1

Hope this helps.

Cheers

Mitch

sebhtml commented 12 years ago

You can clone Ray-TestSuite, Ray and RayPlatform to fix this in code/plugin_Library/LibraryPeakFinder.cpp

The script to test peak finding is located in unit-tests/test_peakFinder.sh (Ray-TestSuite).

Otherwise, I will look into that in a few weeks when I have some time.

On Thu, 2012-03-22 at 19:40 -0700, Mitchell J Stanton-Cook wrote:

Hi Seb,

I'm running - Ray version: 2.0.0-rc4

We still get artificially detected "large peaks".

I've included the metrics:

General

['kmer', '17', '19', '21', '23', '25', '27', '29', '31', '33', '35'] ['c > 100 bp', '143', '147', '145', '142', '137', '124', '128', '132', '134', '126'] ['c > 100 bp total len', '5241893', '5465571', '5354470', '5255806', '5242270', '5257263', '5385963', '5405457', '5327053', '5406021'] ['c > 100 bp av len', '36656', '37180', '36927', '37012', '38264', '42397', '42077', '40950', '39754', '42904'] ['c > 100 bp N50', '124514', '139408', '161895', '161895', '162956', '159457', '164333', '159457', '159457', '159457'] ['c > 100 bp largest', '345228', '345245', '348268', '348294', '349160', '345344', '345370', '345526', '345526', '345439'] ['c > 500 bp', '121', '119', '113', '109', '112', '112', '118', '118', '115', '114'], ['c > 500 bp total len', '5238060', '5460711', '5348486', '5249896', '5237169', '5255489', '5384460', '5402805', '5323604', '5404203'] ['c > 500 bp average len', '43289', '45888', '47331', '48164', '46760', '46924', '45631', '45786', '46292', '47405'] ['c > 500 bp N50', '124514', '139408', '161895', '161895', '162956', '159457', '164333', '159457', '159457', '159457'] ['c > 500 bp largest', '345228', '345245', '348268', '348294', '349160', '345344', '345370', '345526', '345526', '345439'] ['s > 100', '125', '132', '129', '121', '125', '110', '115', '116', '117', '108'], ['s > 100 bp total len', '5245763', '5467786', '5356812', '5260052', '5244192', '5307461', '5432292', '5470661', '5389713', '5478137'] ['s > 100 bp av len', '41966', '41422', '41525', '43471', '41953', '48249', '47237', '47160', '46065', '50723'] ['s > 100 bp N50', '159462', '161919', '171861', '164333', '190540', '186846', '171660', '172749', '171272', '187079'] ['s > 100 bp largest', '345228', '345245', '535229', '535191', '535193', '345724', '345370', '345526', '345526', '345439'] ['s > 500 bp', '103', '104', '97', '88', '100', '98', '105', '102', '98', '96'] ['s > 500 bp total len', '5241930', '5462926', '5350828', '5254142', '5239091', '5305687', '5430789', '5468009', '5386264', '5476319'] ['s > 500 bp av', '50892', '52528', '55163', '59706', '52390', '54139', '51721', '53607', '54961', '57044'] ['s > 500 bp N50', '159462', '161919', '171861', '164333', '190540', '186846', '171660', '172749', '171272', '187079'] ['s > 500 bp largest', '345228', '345245', '535229', '535191', '535193', '345724', '345370', '345526', '345526', '345439']

Scaffolding Stats

['NumberOfNChars', 3870, 2215, 2342, 4246, 1922, 50198, 46329, 65204, 62660, 72116] ['NumberOfNCharRuns', 18, 15, 16, 21, 12, 14, 13, 16, 17, 18] ['MinNChars', 29, 23, 37, 56, 17, 881, 506, 3594, 83, 3474] ['MaxNChars', 390, 312, 252, 363, 357, 4357, 4334, 4458, 4379, 4384] ['AvNChars', 215.0, 147.66666666666666, 146.375, 202.1904761904762, 160.16666666666666, 3585.5714285714284, 3563.769230769231, 4075.25, 3685.8823529411766, 4006.4444444444443]]

Insert Stats (: sepearates multiple)

['meanIns', '344', '350', '351', '351', '351', '351:4422', '351:4426', '351:4426', '351:4424', '351:4423'], ['sdIns', '56', '50', '49', '49', '49', '49:24', '49:20', '49:20', '49:22', '49:24']

I've included the LibraryStatistics and Library0 files for kmer 35:

LibraryNumber: 0 InputFormat: Interleaved,Paired DetectionType: Automatic File: XXXX_proc.fastq NumberOfSequences: 8205596 Distribution: 35/Library0.txt Peak 0 AverageOuterDistance: 351 StandardDeviation: 49 Peak 1 AverageOuterDistance: 4423 StandardDeviation: 24

47 1 54 1 57 1 59 1 65 1 66 1 68 1 69 2 71 3 72 2 74 7 75 5 76 5 77 9 78 3 79 4 80 2 81 5 82 1 83 1 84 4 85 4 86 2 87 6 88 1 89 3 90 3 91 2 92 4 93 3 94 3 95 6 96 3 97 7 98 6 99 4 100 7 101 121 102 113 103 93 104 95 105 112 106 128 107 133 108 104 109 127 110 145 111 178 112 180 113 187 114 193 115 201 116 215 117 208 118 265 119 257 120 273 121 263 122 296 123 344 124 345 125 362 126 375 127 342 128 397 129 446 130 482 131 494 132 513 133 515 134 581 135 609 136 661 137 683 138 663 139 733 140 762 141 806 142 821 143 891 144 961 145 959 146 1046 147 1027 148 1114 149 1075 150 1189 151 1188 152 1225 153 1269 154 1340 155 1423 156 1403 157 1454 158 1505 159 1521 160 1562 161 1598 162 1620 163 1719 164 1710 165 1754 166 1757 167 2012 168 1931 169 1912 170 1982 171 1961 172 2029 173 2075 174 2119 175 2110 176 2147 177 2184 178 2158 179 2248 180 2222 181 2314 182 2346 183 2351 184 2361 185 2318 186 2362 187 2352 188 2379 189 2508 190 2416 191 2508 192 2536 193 2425 194 2375 195 2483 196 2544 197 2413 198 2402 199 2469 200 2509 201 2435 202 2573 203 2428 204 2433 205 2496 206 2510 207 2493 208 2578 209 2531 210 2652 211 2472 212 2421 213 2488 214 2457 215 2382 216 2553 217 2475 218 2456 219 2440 220 2431 221 2343 222 2500 223 2450 224 2377 225 2307 226 2384 227 2463 228 2330 229 2389 230 2362 231 2399 232 2349 233 2388 234 2353 235 2386 236 2284 237 2325 238 2374 239 2287 240 2347 241 2366 242 2269 243 2262 244 2261 245 2242 246 2220 247 2348 248 2197 249 2284 250 2332 251 2214 252 2268 253 2243 254 2143 255 2214 256 2226 257 2234 258 2142 259 2118 260 2192 261 2168 262 2152 263 2126 264 2229 265 2128 266 2162 267 2158 268 2151 269 2136 270 2156 271 2201 272 2200 273 2264 274 2250 275 2223 276 2263 277 2135 278 2231 279 2183 280 2336 281 2233 282 2268 283 2252 284 2308 285 2267 286 2329 287 2340 288 2293 289 2404 290 2272 291 2288 292 2469 293 2391 294 2441 295 2534 296 2448 297 2516 298 2529 299 2425 300 2612 301 2662 302 2732 303 2782 304 2876 305 2952 306 3094 307 3176 308 3301 309 3518 310 3664 311 3986 312 4174 313 4420 314 4805 315 5167 316 5657 317 6207 318 6835 319 7489 320 8414 321 9325 322 10344 323 11486 324 12684 325 14228 326 15928 327 17390 328 19347 329 20962 330 22709 331 24658 332 26985 333 28822 334 31205 335 32772 336 34470 337 36416 338 37924 339 39824 340 40501 341 41865 342 43719 343 44970 344 45913 345 46423 346 47696 347 48126 348 48847 349 49953 350 50303 351 50775 352 51165 353 51567 354 51607 355 52142 356 52288 357 52254 358 52345 359 52346 360 52078 361 51349 362 51441 363 51071 364 50682 365 50937 366 50411 367 50102 368 49922 369 49540 370 48842 371 48466 372 48273 373 47883 374 47479 375 46592 376 46501 377 45421 378 44737 379 44276 380 43646 381 43146 382 41802 383 41218 384 40094 385 39390 386 38392 387 37312 388 36124 389 34988 390 33370 391 32068 392 30878 393 29090 394 27928 395 26226 396 25191 397 23977 398 22608 399 21323 400 20070 401 18912 402 17747 403 16524 404 15490 405 14380 406 13500 407 12759 408 11992 409 11160 410 10352 411 9636 412 9086 413 8313 414 7739 415 7460 416 6908 417 6255 418 5861 419 5610 420 5171 421 4593 422 4294 423 4030 424 3728 425 3496 426 3164 427 2873 428 2653 429 2404 430 2215 431 2005 432 1861 433 1640 434 1485 435 1305 436 1250 437 1062 438 943 439 819 440 745 441 674 442 567 443 515 444 460 445 380 446 336 447 319 448 276 449 207 450 197 451 161 452 138 453 122 454 104 455 100 456 79 457 68 458 52 459 46 460 33 461 31 462 29 463 10 464 19 465 23 466 11 467 14 468 10 469 9 470 12 471 10 472 2 473 2 474 4 475 3 476 4 477 4 478 2 479 1 480 2 481 2 482 3 483 1 484 1 485 2 486 2 488 1 489 1 490 4 493 2 494 1 495 1 497 1 499 3 505 2 506 2 507 2 508 1 511 3 513 1 517 1 520 2 523 1 525 1 528 1 529 1 535 1 537 4 538 1 540 1 544 2 545 3 546 1 549 1 551 1 552 1 554 1 555 1 559 1 561 1 567 1 571 1 572 1 586 1 587 3 592 1 594 1 598 1 604 1 607 1 612 1 621 1 630 1 632 1 636 1 639 1 649 1 655 1 656 1 657 1 670 1 679 1 682 1 683 1 684 1 690 1 693 1 695 2 708 1 713 1 716 1 721 1 732 2 733 1 755 1 779 1 780 1 808 1 895 1 973 1 1302 1 1497 1 2056 1 2237 1 3744 1 3937 1 3981 1 4279 2 4345 1 4350 1 4352 1 4353 1 4355 2 4358 1 4359 1 4361 1 4362 1 4363 2 4365 2 4368 1 4369 2 4370 3 4371 4 4372 2 4373 1 4375 2 4377 1 4379 1 4380 2 4382 2 4384 2 4385 1 4386 1 4387 4 4388 3 4389 2 4390 2 4391 3 4392 4 4393 5 4395 1 4396 1 4397 8 4398 6 4399 2 4401 3 4402 3 4403 5 4404 3 4405 6 4406 6 4407 4 4408 7 4409 4 4410 5 4411 5 4412 5 4413 6 4414 3 4415 4 4416 4 4417 12 4418 3 4419 5 4420 6 4421 6 4422 9 4423 7 4424 6 4425 5 4426 8 4427 8 4428 4 4429 2 4430 2 4431 5 4432 4 4433 5 4434 7 4435 4 4436 7 4437 6 4438 3 4439 3 4440 6 4441 6 4442 5 4443 4 4444 2 4445 6 4446 4 4447 4 4448 4 4449 6 4450 5 4451 4 4452 3 4453 3 4454 7 4455 6 4456 1 4457 2 4458 3 4459 3 4460 3 4461 1 4462 2 4463 2 4464 2 4466 1 4467 1 4468 2 4470 1 4471 1 4474 2 4479 1 4480 1 4484 1 4492 1 4494 1 4496 1 4501 1 4510 1 4511 1 4521 1 4532 1 4536 2 4543 1 4556 1 4561 1 4568 1 4581 1 4583 1 4585 1 4587 1 4597 1 4602 1 4616 1 4637 1 4721 1 4871 1 5126 1 5276 1 5848 1 6751 1 6868 1 7544 1 7996 1 8213 1 8528 1 13467 1 14233 1 16717 1 19963 1 20008 1 32159 1 34585 1 39611 1 40416 1 45612 1

Hope this helps.

Cheers

Mitch

Reply to this email directly or view it on GitHub: https://github.com/sebhtml/ray/issues/49

sebhtml commented 12 years ago

Hello,

I added a unit test for your case in Ray-TestSuite.

The problem was that the signal was being poluted by points with a frequency of only 1.

Therefore, the new code discards frequencies of 1.

All the tests pass for this suite:

[sboisver12@colosse1 unit-tests]$ bash test_peakFinder.sh > log [sboisver12@colosse1 unit-tests]$ grep error log|wc -l 0 [sboisver12@colosse1 unit-tests]$ grep FAIL log|wc -l 0 [sboisver12@colosse1 unit-tests]$ grep PASS log|wc -l 61

Thank you for helping improving the software !

[sboisver12@colosse1 unit-tests]$ ./peaks Libraries/mitch-issue-49.txt Mode= 2 signalAverage= 4 CURRENT IS NOISE, 485 GOT HIT 359 Peak 0 351 49

See this commit https://github.com/sebhtml/ray/commit/538550f8f2648a4b72bce949c9b69975ff76dabc