Open Grelot opened 4 years ago
I want to perform demultiplexing on paired-end fastq files but with barcodes for each pair fastq file.
This basic command works well
$flexbar -r $R1_fastq --barcodes $Tags
This one too
$flexbar -r $R1_fastq -p $R2_fastq --barcodes $Tags
But now i want to check specific barcodes on the second pair:
$flexbar -r $R1_fastq -p $R2_fastq --barcodes $Tags_F --barcodes2 $Tags_R
This command gave me the following output:
________ __ / ____/ /__ _ __/ /_ ____ ______ / /_ / / _ \| |/ / __ \/ __ `/ ___/ / __/ / / __/> </ /_/ / /_/ / / /_/ /_/\___/_/|_/_.___/\__,_/_/ Flexbar - flexible barcode and adapter removal, version 2.5 Local time: Wed Dec 4 14:31:03 2019 Target name: flexbar File type: fastq Reads file: /share/reservebenefit/working/Input_data/Outputs/grinder_teleo1/grinder_teleo1_R1.fastq Reads file 2: /share/reservebenefit/working/Input_data/Outputs/grinder_teleo1/grinder_teleo1_R2.fastq (paired run) Barcode file: /share/reservebenefit/working/pierre/eDNA--benchmark_pipelines/02_demultiplex/Tags_F.fasta Barcode file 2: /share/reservebenefit/working/pierre/eDNA--benchmark_pipelines/02_demultiplex/Tags_R.fasta threads: 1 max-uncalled: 0 min-read-length: 18 barcode-trim-end: ANY barcode-threshold: 1 barcode-match: 1 barcode-mismatch: -1 barcode-gap: -9 Barcode: Sequence: S1-01 ^AACAAGCC S1-02 ^TGAGAGCT S1-03 ^ACAACCGA S1-04 ^TTACGCCA S1-05 ^GGAGAAGA S1-06 ^CAGGCTAA [...] S29-11 ^TCTGTTCG S29-12 ^ACCAGTAC Barcode2: Sequence: S1-01 ^AACAAGCC S1-02 ^TGAGAGCT [...] S29-10 ^CTTGTGAC S29-11 ^TCTGTTCG S29-12 ^ACCAGTAC Processing reads ...Error opening file: flexbar_barcode_S14-06-S1-02_2.fastq
It also generated empty files with name as combination of tags
flexbar_barcode_S1-01-S1-01_1.fastq flexbar_barcode_S1-01-S1-01_2.fastq flexbar_barcode_S1-01-S1-02_1.fastq flexbar_barcode_S1-01-S1-02_2.fastq flexbar_barcode_S1-02-S1-01_1.fastq flexbar_barcode_S1-02-S1-01_2.fastq flexbar_barcode_S1-02-S1-02_1.fastq flexbar_barcode_S1-02-S1-02_2.fastq flexbar_barcode_S1-03-S1-01_1.fastq flexbar_barcode_S1-03-S1-01_2.fastq flexbar_barcode_S1-03-S1-02_1.fastq flexbar_barcode_S1-03-S1-02_2.fastq flexbar_barcode_S1-04-S1-01_1.fastq flexbar_barcode_S1-04-S1-01_2.fastq flexbar_barcode_S1-04-S1-02_1.fastq flexbar_barcode_S1-04-S1-02_2.fastq flexbar_barcode_S1-05-S1-01_1.fastq flexbar_barcode_S1-05-S1-01_2.fastq flexbar_barcode_S1-05-S1-02_1.fastq flexbar_barcode_S1-05-S1-02_2.fastq flexbar_barcode_S1-06-S1-01_1.fastq flexbar_barcode_S1-06-S1-01_2.fastq flexbar_barcode_S1-06-S1-02_1.fastq [...]
Did you got it to work for pair-end?
I want to perform demultiplexing on paired-end fastq files but with barcodes for each pair fastq file.
This basic command works well
This one too
But now i want to check specific barcodes on the second pair:
This command gave me the following output:
It also generated empty files with name as combination of tags