We reanalysed the single cell sequencing data in GSE186527 and the details are attached below. We might missed out some details that we couldn't get similar results.
After the standard 10x analytic pipeline (only without the doublet removing), we found T1 sample have more cells than other samples and we are confused why T1/T2/T3 sample consists of both the unwound and post operative wound samples. We also could not screen out similar scar-associated genes and regeneration-associated genes.
We believed our analystic pipeline has some difference with yours and we wonder whether you could provide the integration code?
question about csc.pdf
Hi Shamik,
We reanalysed the single cell sequencing data in GSE186527 and the details are attached below. We might missed out some details that we couldn't get similar results.
After the standard 10x analytic pipeline (only without the doublet removing), we found T1 sample have more cells than other samples and we are confused why T1/T2/T3 sample consists of both the unwound and post operative wound samples. We also could not screen out similar scar-associated genes and regeneration-associated genes.
We believed our analystic pipeline has some difference with yours and we wonder whether you could provide the integration code?
Looking forward to your reply!