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shandley / hecatomb

hecatomb is a virome analysis pipeline for analysis of Illumina sequence data
MIT License
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HPC database installation problems #86

Closed katievigil closed 6 months ago

katievigil commented 1 year ago

Hi! I have an issue downloading the databases for HPC:

Looks like my install worked but I can't download the databases for HPC:

(/lustre/project/taw/share/conda-envs/hecatomb) [kvigil@cypress2 conda-envs]$ hecatomb --help

██╗ ██╗███████╗ ██████╗ █████╗ ████████╗ ██████╗ ███╗ ███╗██████╗ ██║ ██║██╔════╝██╔════╝██╔══██╗╚══██╔══╝██╔═══██╗████╗ ████║██╔══██╗ ███████║█████╗ ██║ ███████║ ██║ ██║ ██║██╔████╔██║██████╔╝ ██╔══██║██╔══╝ ██║ ██╔══██║ ██║ ██║ ██║██║╚██╔╝██║██╔══██╗ ██║ ██║███████╗╚██████╗██║ ██║ ██║ ╚██████╔╝██║ ╚═╝ ██║██████╔╝ ╚═╝ ╚═╝╚══════╝ ╚═════╝╚═╝ ╚═╝ ╚═╝ ╚═════╝ ╚═╝ ╚═╝╚═════╝

Hecatomb version v1.1.0

Usage: hecatomb [OPTIONS] COMMAND [ARGS]...

Subcommand usage: hecatomb subcommand --help

Options: --help Show this message and exit.

Commands: run Run Hecatomb test Run the Hecatomb test dataset config Copy the system default config file install Install the Hecatomb databases combine Combine multiple Hecatomb runs add-host Add a new host genome to use with Hecatomb list-hosts List the available host genomes (/lustre/project/taw/share/conda-envs/hecatomb) [kvigil@cypress2 conda-envs]$

(/lustre/project/taw/share/conda-envs/hecatomb) [kvigil@cypress2 conda-envs]$ hecatomb install --profile slurm

██╗ ██╗███████╗ ██████╗ █████╗ ████████╗ ██████╗ ███╗ ███╗██████╗ ██║ ██║██╔════╝██╔════╝██╔══██╗╚══██╔══╝██╔═══██╗████╗ ████║██╔══██╗ ███████║█████╗ ██║ ███████║ ██║ ██║ ██║██╔████╔██║██████╔╝ ██╔══██║██╔══╝ ██║ ██╔══██║ ██║ ██║ ██║██║╚██╔╝██║██╔══██╗ ██║ ██║███████╗╚██████╗██║ ██║ ██║ ╚██████╔╝██║ ╚═╝ ██║██████╔╝ ╚═╝ ╚═╝╚══════╝ ╚═════╝╚═╝ ╚═╝ ╚═╝ ╚═════╝ ╚═╝ ╚═╝╚═════╝

Hecatomb version v1.1.0

[2023:01:25 08:33:58] Config file hecatomb.config.yaml already exists. Using existing config file. [2023:01:25 08:33:58] Writing runtime config file to hecatomb.config.yaml [2023:01:25 08:33:58] ------------------ [2023:01:25 08:33:58] | Runtime config | [2023:01:25 08:33:58] ------------------

BigJobCpu: 24 BigJobMem: 64000 BigJobTimeMin: 1440 COMPRESSION: 1 CONTIG_MINLENGTH: 1000 CUTTAIL_WINDOW: 25 DEDUP_ACCURACY: 4 Databases: null ENTROPY: 0.5 ENTROPYWINDOW: 25 Host: human MediumJobCpu: 16 MediumJobMem: 32000 MoreRamCpu: 2 MoreRamMem: 16000 Output: hecatomb.out Preprocess: paired QSCORE: 15 READ_MINLENGTH: 90 Reads: /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data Search: sensitive SmallJobCpu: 8 SmallJobMem: 16000 canuSettings: correctedErrorRate=0.16 maxInputCoverage=10000 minInputCoverage=0 corOutCoverage=10000 corMhapSensitivity=high corMinCoverage=0 useGrid=False stopOnLowCoverage=False genomeSize=10M -nanopore filtAAprimary: --min-length 30 -e 1e-3 filtAAsecondary: --min-length 30 -e 1e-5 filtNTprimary: --min-length 90 -e 1e-10 filtNTsecondary: --min-length 90 -e 1e-20 linclustParams: --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3 perfAA: --start-sens 1 --sens-steps 3 -s 7 --lca-mode 2 --shuffle 0 perfAAfast: -s 4.0 --lca-mode 2 --shuffle 0 perfNT: --start-sens 2 -s 7 --sens-steps 3 perfNTfast: -s 4.0 taxIdIgnore: 0 1 2 10239 131567 12429 2759

[2023:01:25 08:33:58] --------------------- [2023:01:25 08:33:58] | Snakemake command | [2023:01:25 08:33:58] ---------------------

snakemake -s /lustre/project/taw/share/conda-envs/hecatomb/bin/../snakemake/workflow/DownloadDB.smk --configfile hecatomb.config.yaml --rerun-incomplete --printshellcmds --nolock --show-failed-logs --profile slurm Error: profile given but no config.yaml found. Profile has to be given as either absolute path, relative path or name of a directory available in either /etc/xdg/snakemake or /home/kvigil/.config/snakemake. [2023:01:25 08:34:08] Error: Snakemake failed

2023-01-24T152543.473897.snakemake.log

2023-01-25T071632.241265.snakemake.log

Thanks!

katievigil commented 1 year ago

Update- I read the wiki and created a snakemake profile. I tried to run the install for HPC and get an error, see below.

Thanks!

(/lustre/project/taw/share/conda-envs/hecatomb) [kvigil@cypress2 slurm]$ hecatomb install --profile slurm

██╗ ██╗███████╗ ██████╗ █████╗ ████████╗ ██████╗ ███╗ ███╗██████╗ ██║ ██║██╔════╝██╔════╝██╔══██╗╚══██╔══╝██╔═══██╗████╗ ████║██╔══██╗ ███████║█████╗ ██║ ███████║ ██║ ██║ ██║██╔████╔██║██████╔╝ ██╔══██║██╔══╝ ██║ ██╔══██║ ██║ ██║ ██║██║╚██╔╝██║██╔══██╗ ██║ ██║███████╗╚██████╗██║ ██║ ██║ ╚██████╔╝██║ ╚═╝ ██║██████╔╝ ╚═╝ ╚═╝╚══════╝ ╚═════╝╚═╝ ╚═╝ ╚═╝ ╚═════╝ ╚═╝ ╚═╝╚═════╝

Hecatomb version v1.1.0

[2023:01:26 16:07:35] Config file hecatomb.config.yaml already exists. Using existing config file. [2023:01:26 16:07:35] Writing runtime config file to hecatomb.config.yaml [2023:01:26 16:07:35] ------------------ [2023:01:26 16:07:35] | Runtime config | [2023:01:26 16:07:35] ------------------

BigJobCpu: 24 BigJobMem: 64000 BigJobTimeMin: 1440 COMPRESSION: 1 CONTIG_MINLENGTH: 1000 CUTTAIL_WINDOW: 25 DEDUP_ACCURACY: 4 Databases: null ENTROPY: 0.5 ENTROPYWINDOW: 25 Host: human MediumJobCpu: 16 MediumJobMem: 32000 MoreRamCpu: 2 MoreRamMem: 16000 Output: hecatomb.out Preprocess: paired QSCORE: 15 READ_MINLENGTH: 90 Reads: /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data Search: sensitive SmallJobCpu: 8 SmallJobMem: 16000 canuSettings: correctedErrorRate=0.16 maxInputCoverage=10000 minInputCoverage=0 corOutCoverage=10000 corMhapSensitivity=high corMinCoverage=0 useGrid=False stopOnLowCoverage=False genomeSize=10M -nanopore filtAAprimary: --min-length 30 -e 1e-3 filtAAsecondary: --min-length 30 -e 1e-5 filtNTprimary: --min-length 90 -e 1e-10 filtNTsecondary: --min-length 90 -e 1e-20 linclustParams: --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3 perfAA: --start-sens 1 --sens-steps 3 -s 7 --lca-mode 2 --shuffle 0 perfAAfast: -s 4.0 --lca-mode 2 --shuffle 0 perfNT: --start-sens 2 -s 7 --sens-steps 3 perfNTfast: -s 4.0 taxIdIgnore: 0 1 2 10239 131567 12429 2759

[2023:01:26 16:07:35] --------------------- [2023:01:26 16:07:35] | Snakemake command | [2023:01:26 16:07:35] ---------------------

snakemake -s /lustre/project/taw/share/conda-envs/hecatomb/bin/../snakemake/workflow/DownloadDB.smk --configfile hecatomb.config.yaml --rerun-incomplete --printshellcmds --nolock --show-failed-logs --profile slurm Config file /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../config/config.yaml is extended by additional config specified via the command line. Config file /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../config/dbFiles.yaml is extended by additional config specified via the command line. Building DAG of jobs... Using shell: /bin/bash Provided cluster nodes: 100 Job stats: job count min threads max threads

all 1 1 1 download_db_file 7 1 1 total 8 1 1

Select jobs to execute...

[Thu Jan 26 16:07:39 2023] rule download_db_file: output: /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/nt/virus_primary_nt/sequenceDB jobid: 45 reason: Forced execution wildcards: file=nt/virus_primary_nt/sequenceDB resources: mem_mb=2000, mem_mib=1908, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

Traceback (most recent call last): File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/exceptions.py", line 1079, in call return func(*args, **kwargs) File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/cli/main.py", line 76, in _main init_loggers(context) File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/cli/main.py", line 58, in init_loggers if context and context.json: File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/common/configuration.py", line 1207, in get matches = [self.type.load(self.name, match) for match in raw_matches] File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/common/configuration.py", line 1207, in matches = [self.type.load(self.name, match) for match in raw_matches] File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/common/configuration.py", line 974, in load match.value(self._element_type), File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/common/configuration.py", line 267, in value return make_immutable(self._raw_value) File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/_vendor/auxlib/collection.py", line 24, in make_immutable elif isiterable(value): File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/_vendor/auxlib/compat.py", line 37, in isiterable return not isinstance(obj, string_types) and isinstance(obj, collections.Iterable) AttributeError: module 'collections' has no attribute 'Iterable'

During handling of the above exception, another exception occurred:

Traceback (most recent call last): File "/lustre/project/taw/share/conda-envs/hecatomb/bin/conda", line 13, in sys.exit(main()) File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/cli/main.py", line 150, in main return conda_exception_handler(_main, *args, *kwargs) File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/exceptions.py", line 1371, in conda_exception_handler return_value = exception_handler(func, args, kwargs) File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/exceptions.py", line 1082, in call return self.handle_exception(exc_val, exc_tb) File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/exceptions.py", line 1126, in handle_exception return self.handle_unexpected_exception(exc_val, exc_tb) File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/exceptions.py", line 1137, in handle_unexpected_exception self.print_unexpected_error_report(error_report) File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/exceptions.py", line 1193, in print_unexpected_error_report if context.json: File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/common/configuration.py", line 1207, in get matches = [self.type.load(self.name, match) for match in raw_matches] File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/common/configuration.py", line 1207, in matches = [self.type.load(self.name, match) for match in raw_matches] File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/common/configuration.py", line 974, in load match.value(self._element_type), File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/common/configuration.py", line 267, in value return make_immutable(self._raw_value) File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/_vendor/auxlib/collection.py", line 24, in make_immutable elif isiterable(value): File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/_vendor/auxlib/compat.py", line 37, in isiterable return not isinstance(obj, string_types) and isinstance(obj, collections.Iterable) AttributeError: module 'collections' has no attribute 'Iterable' Traceback (most recent call last): File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/site-packages/snakemake/init.py", line 757, in snakemake success = workflow.execute( File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/site-packages/snakemake/workflow.py", line 1089, in execute raise e File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/site-packages/snakemake/workflow.py", line 1085, in execute success = self.scheduler.schedule() File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/site-packages/snakemake/scheduler.py", line 592, in schedule self.run(runjobs) File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/site-packages/snakemake/scheduler.py", line 641, in run executor.run_jobs( File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/site-packages/snakemake/executors/init.py", line 155, in run_jobs self.run( File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/site-packages/snakemake/executors/init.py", line 1156, in run self.write_jobscript(job, jobscript) File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/site-packages/snakemake/executors/init.py", line 884, in write_jobscript exec_job = self.format_job_exec(job) File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/site-packages/snakemake/executors/init.py", line 442, in format_job_exec self.general_args, File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/site-packages/snakemake/common/init.py", line 218, in get value = self.method(instance) File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/site-packages/snakemake/executors/init.py", line 334, in general_args w2a("conda_base_path", skip=not self.assume_shared_fs), File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/site-packages/snakemake/executors/init.py", line 294, in workflow_property_to_arg value = getattr(self.workflow, property) File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/site-packages/snakemake/workflow.py", line 300, in conda_base_path return Conda().prefix_path File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/site-packages/snakemake/deployment/conda.py", line 667, in init shell.check_output(self._get_cmd(f"conda info --json"), text=True) File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/site-packages/snakemake/shell.py", line 63, in check_output return sp.check_output(cmd, shell=True, executable=executable, kwargs) File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/subprocess.py", line 421, in check_output return run(*popenargs, stdout=PIPE, timeout=timeout, check=True, File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/subprocess.py", line 526, in run raise CalledProcessError(retcode, process.args, subprocess.CalledProcessError: Command 'conda info --json' returned non-zero exit status 1. [2023:01:26 16:07:41] Error: Snakemake failed

beardymcjohnface commented 1 year ago

This is a weird issue and I'm guessing there's something wrong with the environment. Did you load anaconda as a module on your HPC? collections.iterable was deprecated in 3.10 and it looks like the workflow is calling packages from both 3.8 and 3.10. I would suggest trying a fresh install of miniconda on your home directory with python 3.10 if that's possible.

katievigil commented 1 year ago

Looks like it worked when I load the module right before running! Thanks

(base) [kvigil@cypress1 ~]$ module load anaconda3/2020.07 (base) [kvigil@cypress1 ~]$ conda activate hecatomb (/lustre/project/taw/share/conda-envs/hecatomb) [kvigil@cypress1 ~]$ hecatomb install --profile slurm

██╗ ██╗███████╗ ██████╗ █████╗ ████████╗ ██████╗ ███╗ ███╗██████╗ ██║ ██║██╔════╝██╔════╝██╔══██╗╚══██╔══╝██╔═══██╗████╗ ████║██╔══██╗ ███████║█████╗ ██║ ███████║ ██║ ██║ ██║██╔████╔██║██████╔╝ ██╔══██║██╔══╝ ██║ ██╔══██║ ██║ ██║ ██║██║╚██╔╝██║██╔══██╗ ██║ ██║███████╗╚██████╗██║ ██║ ██║ ╚██████╔╝██║ ╚═╝ ██║██████╔╝ ╚═╝ ╚═╝╚══════╝ ╚═════╝╚═╝ ╚═╝ ╚═╝ ╚═════╝ ╚═╝ ╚═╝╚═════╝

Hecatomb version v1.1.0

[2023:01:27 07:37:56] Config file hecatomb.config.yaml already exists. Using existing config file. [2023:01:27 07:37:56] Writing runtime config file to hecatomb.config.yaml [2023:01:27 07:37:56] ------------------ [2023:01:27 07:37:56] | Runtime config | [2023:01:27 07:37:56] ------------------

BigJobCpu: 24 BigJobMem: 64000 BigJobTimeMin: 1440 COMPRESSION: 1 CONTIG_MINLENGTH: 1000 CUTTAIL_WINDOW: 25 DEDUP_ACCURACY: 4 Databases: null ENTROPY: 0.5 ENTROPYWINDOW: 25 Host: human MediumJobCpu: 16 MediumJobMem: 32000 MoreRamCpu: 2 MoreRamMem: 16000 Output: hecatomb.out Preprocess: paired QSCORE: 15 READ_MINLENGTH: 90 Reads: /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data Search: sensitive SmallJobCpu: 8 SmallJobMem: 16000 canuSettings: correctedErrorRate=0.16 maxInputCoverage=10000 minInputCoverage=0 corOutCoverage=10000 corMhapSensitivity=high corMinCoverage=0 useGrid=False stopOnLowCoverage=False genomeSize=10M -nanopore filtAAprimary: --min-length 30 -e 1e-3 filtAAsecondary: --min-length 30 -e 1e-5 filtNTprimary: --min-length 90 -e 1e-10 filtNTsecondary: --min-length 90 -e 1e-20 linclustParams: --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3 perfAA: --start-sens 1 --sens-steps 3 -s 7 --lca-mode 2 --shuffle 0 perfAAfast: -s 4.0 --lca-mode 2 --shuffle 0 perfNT: --start-sens 2 -s 7 --sens-steps 3 perfNTfast: -s 4.0 taxIdIgnore: 0 1 2 10239 131567 12429 2759

[2023:01:27 07:37:56] --------------------- [2023:01:27 07:37:56] | Snakemake command | [2023:01:27 07:37:56] ---------------------

snakemake -s /lustre/project/taw/share/conda-envs/hecatomb/bin/../snakemake/workflow/DownloadDB.smk --configfile hecatomb.config.yaml --rerun-incomplete --printshellcmds --nolock --show-failed-logs --profile slurm Config file /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../config/config.yaml is extended by additional config specified via the command line. Config file /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../config/dbFiles.yaml is extended by additional config specified via the command line. Building DAG of jobs... Nothing to be done (all requested files are present and up to date). Complete log: .snakemake/log/2023-01-27T073806.383518.snakemake.log [2023:01:27 07:38:09] Snakemake finished successfully

katievigil commented 1 year ago

So looks like when I run the HPC test I get a snakemake failed error:

(/lustre/project/taw/share/conda-envs/hecatomb) [kvigil@cypress1 ~]$ hecatomb test --profile slurm

██╗ ██╗███████╗ ██████╗ █████╗ ████████╗ ██████╗ ███╗ ███╗██████╗ ██║ ██║██╔════╝██╔════╝██╔══██╗╚══██╔══╝██╔═══██╗████╗ ████║██╔══██╗ ███████║█████╗ ██║ ███████║ ██║ ██║ ██║██╔████╔██║██████╔╝ ██╔══██║██╔══╝ ██║ ██╔══██║ ██║ ██║ ██║██║╚██╔╝██║██╔══██╗ ██║ ██║███████╗╚██████╗██║ ██║ ██║ ╚██████╔╝██║ ╚═╝ ██║██████╔╝ ╚═╝ ╚═╝╚══════╝ ╚═════╝╚═╝ ╚═╝ ╚═╝ ╚═════╝ ╚═╝ ╚═╝╚═════╝

Hecatomb version v1.1.0

[2023:01:27 07:40:59] Config file hecatomb.config.yaml already exists. Using existing config file. [2023:01:27 07:40:59] Writing runtime config file to hecatomb.config.yaml [2023:01:27 07:40:59] ------------------ [2023:01:27 07:40:59] | Runtime config | [2023:01:27 07:40:59] ------------------

BigJobCpu: 24 BigJobMem: 64000 BigJobTimeMin: 1440 COMPRESSION: 1 CONTIG_MINLENGTH: 1000 CUTTAIL_WINDOW: 25 DEDUP_ACCURACY: 4 Databases: null ENTROPY: 0.5 ENTROPYWINDOW: 25 Host: human MediumJobCpu: 16 MediumJobMem: 32000 MoreRamCpu: 2 MoreRamMem: 16000 Output: hecatomb.out Preprocess: paired QSCORE: 15 READ_MINLENGTH: 90 Reads: /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data Search: sensitive SmallJobCpu: 8 SmallJobMem: 16000 canuSettings: correctedErrorRate=0.16 maxInputCoverage=10000 minInputCoverage=0 corOutCoverage=10000 corMhapSensitivity=high corMinCoverage=0 useGrid=False stopOnLowCoverage=False genomeSize=10M -nanopore filtAAprimary: --min-length 30 -e 1e-3 filtAAsecondary: --min-length 30 -e 1e-5 filtNTprimary: --min-length 90 -e 1e-10 filtNTsecondary: --min-length 90 -e 1e-20 linclustParams: --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3 perfAA: --start-sens 1 --sens-steps 3 -s 7 --lca-mode 2 --shuffle 0 perfAAfast: -s 4.0 --lca-mode 2 --shuffle 0 perfNT: --start-sens 2 -s 7 --sens-steps 3 perfNTfast: -s 4.0 taxIdIgnore: 0 1 2 10239 131567 12429 2759

[2023:01:27 07:40:59] --------------------- [2023:01:27 07:40:59] | Snakemake command | [2023:01:27 07:40:59] ---------------------

snakemake -s /lustre/project/taw/share/conda-envs/hecatomb/bin/../snakemake/workflow/Hecatomb.smk --configfile hecatomb.config.yaml --use-conda --conda-frontend mamba --conda-prefix /lustre/project/taw/share/conda-envs/hecatomb/bin/../snakemake/conda --rerun-incomplete --printshellcmds --nolock --show-failed-logs --profile slurm Config file /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../config/config.yaml is extended by additional config specified via the command line. Config file /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../config/dbFiles.yaml is extended by additional config specified via the command line. Config file /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../config/immutable.yaml is extended by additional config specified via the command line. Building DAG of jobs... Traceback (most recent call last): File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/exceptions.py", line 1079, in call return func(*args, **kwargs) File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/cli/main.py", line 76, in _main init_loggers(context) File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/cli/main.py", line 58, in init_loggers if context and context.json: File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/common/configuration.py", line 1207, in get matches = [self.type.load(self.name, match) for match in raw_matches] File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/common/configuration.py", line 1207, in matches = [self.type.load(self.name, match) for match in raw_matches] File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/common/configuration.py", line 974, in load match.value(self._element_type), File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/common/configuration.py", line 267, in value return make_immutable(self._raw_value) File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/_vendor/auxlib/collection.py", line 24, in make_immutable elif isiterable(value): File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/_vendor/auxlib/compat.py", line 37, in isiterable return not isinstance(obj, string_types) and isinstance(obj, collections.Iterable) AttributeError: module 'collections' has no attribute 'Iterable'

During handling of the above exception, another exception occurred:

Traceback (most recent call last): File "/lustre/project/taw/share/conda-envs/hecatomb/bin/conda", line 13, in sys.exit(main()) File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/cli/main.py", line 150, in main return conda_exception_handler(_main, *args, *kwargs) File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/exceptions.py", line 1371, in conda_exception_handler return_value = exception_handler(func, args, kwargs) File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/exceptions.py", line 1082, in call return self.handle_exception(exc_val, exc_tb) File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/exceptions.py", line 1126, in handle_exception return self.handle_unexpected_exception(exc_val, exc_tb) File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/exceptions.py", line 1137, in handle_unexpected_exception self.print_unexpected_error_report(error_report) File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/exceptions.py", line 1193, in print_unexpected_error_report if context.json: File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/common/configuration.py", line 1207, in get matches = [self.type.load(self.name, match) for match in raw_matches] File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/common/configuration.py", line 1207, in matches = [self.type.load(self.name, match) for match in raw_matches] File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/common/configuration.py", line 974, in load match.value(self._element_type), File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/common/configuration.py", line 267, in value return make_immutable(self._raw_value) File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/_vendor/auxlib/collection.py", line 24, in make_immutable elif isiterable(value): File "/share/apps/anaconda/3/2020.07/lib/python3.8/site-packages/conda/_vendor/auxlib/compat.py", line 37, in isiterable return not isinstance(obj, string_types) and isinstance(obj, collections.Iterable) AttributeError: module 'collections' has no attribute 'Iterable' Traceback (most recent call last): File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/site-packages/snakemake/init.py", line 757, in snakemake success = workflow.execute( File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/site-packages/snakemake/workflow.py", line 928, in execute dag.create_conda_envs( File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/site-packages/snakemake/dag.py", line 338, in create_conda_envs env.create(dryrun) File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/site-packages/snakemake/deployment/conda.py", line 405, in create pin_file = self.pin_file File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/site-packages/snakemake/common/init.py", line 218, in get value = self.method(instance) File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/site-packages/snakemake/deployment/conda.py", line 109, in pin_file f".{self.conda.platform}.pin.txt" File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/site-packages/snakemake/common/init.py", line 218, in get value = self.method(instance) File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/site-packages/snakemake/deployment/conda.py", line 102, in conda return Conda( File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/site-packages/snakemake/deployment/conda.py", line 667, in init shell.check_output(self._get_cmd(f"conda info --json"), text=True) File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/site-packages/snakemake/shell.py", line 63, in check_output return sp.check_output(cmd, shell=True, executable=executable, kwargs) File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/subprocess.py", line 421, in check_output return run(*popenargs, stdout=PIPE, timeout=timeout, check=True, File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/subprocess.py", line 526, in run raise CalledProcessError(retcode, process.args, subprocess.CalledProcessError: Command 'conda info --json' returned non-zero exit status 1. [2023:01:27 07:41:06] Error: Snakemake failed

katievigil commented 1 year ago

2023-01-27T102534.391649.snakemake.log 2023-01-27T102517.189801.snakemake.log 2023-01-27T102344.153011.snakemake.log 2023-01-27T102208.438015.snakemake.log 2023-01-27T102146.386328.snakemake.log 2023-01-27T102120.713968.snakemake.log 2023-01-27T074100.928790.snakemake.log 2023-01-27T073806.383518.snakemake.log

katievigil commented 1 year ago

Hi did anybody checkout these logs? Thanks katie

beardymcjohnface commented 1 year ago

Hi, Sorry I meant to follow up on this. I'm not sure which of those logs is the latest, but based on what you copy pasted it looks like the same issue as before and I think the anaconda module might be too old. I would try installing miniconda with python 3.10. You should be able to install a local copy to your home directory even on an HPC.

katievigil commented 1 year ago

@beardymcjohnface Hi thank you! I will definitely try this out. Thanks for the response.

katievigil commented 1 year ago

Hi I am still getting a snakemake error. Also, it doesn't look like this run was saved in a log like it stated below. Thanks!

(/lustre/project/taw/share/conda-envs/hecatomb) [kvigil@cypress2 conda-envs]$ unset PYTHONPATH (/lustre/project/taw/share/conda-envs/hecatomb) [kvigil@cypress2 conda-envs]$ hecatomb test --profile slurm

██╗ ██╗███████╗ ██████╗ █████╗ ████████╗ ██████╗ ███╗ ███╗██████╗ ██║ ██║██╔════╝██╔════╝██╔══██╗╚══██╔══╝██╔═══██╗████╗ ████║██╔══██╗ ███████║█████╗ ██║ ███████║ ██║ ██║ ██║██╔████╔██║██████╔╝ ██╔══██║██╔══╝ ██║ ██╔══██║ ██║ ██║ ██║██║╚██╔╝██║██╔══██╗ ██║ ██║███████╗╚██████╗██║ ██║ ██║ ╚██████╔╝██║ ╚═╝ ██║██████╔╝ ╚═╝ ╚═╝╚══════╝ ╚═════╝╚═╝ ╚═╝ ╚═╝ ╚═════╝ ╚═╝ ╚═╝╚═════╝

Hecatomb version v1.1.0

[2023:03:06 13:20:24] Config file hecatomb.config.yaml already exists. Using existing config file. [2023:03:06 13:20:24] Writing runtime config file to hecatomb.config.yaml [2023:03:06 13:20:24] ------------------ [2023:03:06 13:20:24] | Runtime config | [2023:03:06 13:20:24] ------------------

BigJobCpu: 24 BigJobMem: 64000 BigJobTimeMin: 1440 COMPRESSION: 1 CONTIG_MINLENGTH: 1000 CUTTAIL_WINDOW: 25 DEDUP_ACCURACY: 4 Databases: null ENTROPY: 0.5 ENTROPYWINDOW: 25 Host: human MediumJobCpu: 16 MediumJobMem: 32000 MoreRamCpu: 2 MoreRamMem: 16000 Output: hecatomb.out Preprocess: paired QSCORE: 15 READ_MINLENGTH: 90 Reads: /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data Search: sensitive SmallJobCpu: 8 SmallJobMem: 16000 canuSettings: correctedErrorRate=0.16 maxInputCoverage=10000 minInputCoverage=0 corOutCoverage=10000 corMhapSensitivity=high corMinCoverage=0 useGrid=False stopOnLowCoverage=False genomeSize=10M -nanopore filtAAprimary: --min-length 30 -e 1e-3 filtAAsecondary: --min-length 30 -e 1e-5 filtNTprimary: --min-length 90 -e 1e-10 filtNTsecondary: --min-length 90 -e 1e-20 linclustParams: --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3 perfAA: --start-sens 1 --sens-steps 3 -s 7 --lca-mode 2 --shuffle 0 perfAAfast: -s 4.0 --lca-mode 2 --shuffle 0 perfNT: --start-sens 2 -s 7 --sens-steps 3 perfNTfast: -s 4.0 taxIdIgnore: 0 1 2 10239 131567 12429 2759

[2023:03:06 13:20:24] --------------------- [2023:03:06 13:20:24] | Snakemake command | [2023:03:06 13:20:24] ---------------------

snakemake -s /lustre/project/taw/share/conda-envs/hecatomb/bin/../snakemake/workflow/Hecatomb.smk --configfile hecatomb.config.yaml --use-conda --conda-frontend mamba --conda-prefix /lustre/project/taw/share/conda-envs/hecatomb/bin/../snakemake/conda --rerun-incomplete --printshellcmds --nolock --show-failed-logs --profile slurm Config file /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../config/config.yaml is extended by additional config specified via the command line. Config file /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../config/dbFiles.yaml is extended by additional config specified via the command line. Config file /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../config/immutable.yaml is extended by additional config specified via the command line. Building DAG of jobs... Your conda installation is not configured to use strict channel priorities. This is however crucial for having robust and correct environments (for details, see https://conda-forge.org/docs/user/tipsandtricks.html). Please consider to configure strict priorities by executing 'conda config --set channel_priority strict'. Using shell: /bin/bash Provided cluster nodes: 100 Job stats: job count min threads max threads

PRIMARY_AA_parsing 1 2 2 PRIMARY_AA_taxonomy_assignment 1 24 24 PRIMARY_NT_parsing 1 2 2 PRIMARY_NT_reformat 1 2 2 PRIMARY_NT_taxonomic_assignment 1 24 24 SECONDARY_AA_generate_output_table 1 2 2 SECONDARY_AA_parsing 1 2 2 SECONDARY_AA_refactor_finalize 1 2 2 SECONDARY_AA_taxonomy_assignment 1 24 24 SECONDARY_AA_tophit_lineage 1 2 2 SECONDARY_NT_convert 1 24 24 SECONDARY_NT_generate_output_table 1 2 2 SECONDARY_NT_summary 1 2 2 SECONDARY_NT_taxonomic_assignment 1 24 24 all 1 1 1 archive_for_assembly 10 1 1 assembly_kmer_normalization 10 8 8 bam_index 1 2 2 calculate_gc 2 2 2 calculate_tet_freq 2 2 2 cluster_similar_sequences 10 24 24 combine_AA_NT 1 1 1 concatenate_contigs 1 1 1 concatentate_contig_count_tables 1 1 1 contig_krona_plot 1 1 1 contig_krona_text_format 1 1 1 contig_read_taxonomy 1 2 2 coverage_calculations 10 8 8 create_contig_count_table 10 1 1 create_host_index 1 8 8 create_individual_seqtables 10 24 24 dumpSamplesTsv 1 1 1 fastp_preprocessing 4 16 16 host_removal_mapping 10 8 8 individual_sample_assembly 10 16 16 krona_plot 1 1 1 krona_text_format 1 1 1 link_assembly 1 1 1 mapSampleAssemblyPairedReads 10 8 8 mapSampleAssemblyUnpairedReads 10 8 8 map_seq_table 1 16 16 merge_seq_table 1 1 1 mmseqs_contig_annotation 1 24 24 mmseqs_contig_annotation_summary 1 24 24 nonhost_read_combine 10 1 1 nonhost_read_repair 10 8 8 poolR1Unmapped 1 1 1 poolR2Unmapped 1 1 1 poolUnpairedUnmapped 1 1 1 population_assembly 1 16 16 pullPairedUnmappedReads 10 8 8 pullPairedUnmappedReadsMateMapped 10 8 8 rescue_read_kmer_normalization 1 24 24 secondary_nt_calc_lca 1 24 24 secondary_nt_lca_table 1 1 1 seq_properties_table 2 2 2 tax_level_counts 1 2 2 unmapped_read_rescue_assembly 1 16 16 zip_fastq 60 1 1 total 250 1 24

Select jobs to execute...

[Mon Mar 6 13:20:34 2023] rule fastp_preprocessing: input: /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-258-124-06_CGTACG_R1.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-258-124-06_CGTACG_R2.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/contaminants/vector_contaminants.fa output: hecatomb.out/processing/temp/p01/A13-258-124-06_CGTACG_R1.s1.out.fastq, hecatomb.out/processing/temp/p01/A13-258-124-06_CGTACG_R2.s1.out.fastq, hecatomb.out/processing/stats/p01/A13-258-124-06_CGTACG.s1.stats.json, hecatomb.out/processing/stats/p01/A13-258-124-06_CGTACG.s1.stats.html log: hecatomb.out/stderr/fastp_preprocessing.A13-258-124-06_CGTACG.log jobid: 43 benchmark: hecatomb.out/benchmarks/fastp_preprocessing.A13-258-124-06_CGTACG.txt reason: Missing output files: hecatomb.out/processing/temp/p01/A13-258-124-06_CGTACG_R2.s1.out.fastq, hecatomb.out/processing/temp/p01/A13-258-124-06_CGTACG_R1.s1.out.fastq wildcards: sample=A13-258-124-06_CGTACG threads: 16 resources: mem_mb=32000, mem_mib=30518, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    fastp -i /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-258-124-06_CGTACG_R1.fastq.gz -I /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-258-124-06_CGTACG_R2.fastq.gz -o hecatomb.out/processing/temp/p01/A13-258-124-06_CGTACG_R1.s1.out.fastq -O hecatomb.out/processing/temp/p01/A13-258-124-06_CGTACG_R2.s1.out.fastq             -z 1             -j hecatomb.out/processing/stats/p01/A13-258-124-06_CGTACG.s1.stats.json -h hecatomb.out/processing/stats/p01/A13-258-124-06_CGTACG.s1.stats.html             --qualified_quality_phred 15             --length_required 90             --detect_adapter_for_pe             --cut_tail --cut_tail_window_size 25 --cut_tail_mean_quality 15             --dedup --dup_calc_accuracy 4             --trim_poly_x             --thread 16 2> hecatomb.out/stderr/fastp_preprocessing.A13-258-124-06_CGTACG.log
    rm hecatomb.out/stderr/fastp_preprocessing.A13-258-124-06_CGTACG.log

Submitted job 43 with external jobid 'Submitted batch job 2287003'.

[Mon Mar 6 13:20:34 2023] rule dumpSamplesTsv: output: hecatomb.out/results/hecatomb.samples.tsv jobid: 252 reason: Missing output files: hecatomb.out/results/hecatomb.samples.tsv resources: mem_mb=2000, mem_mib=1908, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

Submitted job 252 with external jobid 'Submitted batch job 2287004'.

[Mon Mar 6 13:20:34 2023] rule fastp_preprocessing: input: /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-117-06_ACTGAT_R1.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-117-06_ACTGAT_R2.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/contaminants/vector_contaminants.fa output: hecatomb.out/processing/temp/p01/A13-256-117-06_ACTGAT_R1.s1.out.fastq, hecatomb.out/processing/temp/p01/A13-256-117-06_ACTGAT_R2.s1.out.fastq, hecatomb.out/processing/stats/p01/A13-256-117-06_ACTGAT.s1.stats.json, hecatomb.out/processing/stats/p01/A13-256-117-06_ACTGAT.s1.stats.html log: hecatomb.out/stderr/fastp_preprocessing.A13-256-117-06_ACTGAT.log jobid: 49 benchmark: hecatomb.out/benchmarks/fastp_preprocessing.A13-256-117-06_ACTGAT.txt reason: Missing output files: hecatomb.out/processing/temp/p01/A13-256-117-06_ACTGAT_R2.s1.out.fastq, hecatomb.out/processing/temp/p01/A13-256-117-06_ACTGAT_R1.s1.out.fastq wildcards: sample=A13-256-117-06_ACTGAT threads: 16 resources: mem_mb=32000, mem_mib=30518, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    fastp -i /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-117-06_ACTGAT_R1.fastq.gz -I /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-117-06_ACTGAT_R2.fastq.gz -o hecatomb.out/processing/temp/p01/A13-256-117-06_ACTGAT_R1.s1.out.fastq -O hecatomb.out/processing/temp/p01/A13-256-117-06_ACTGAT_R2.s1.out.fastq             -z 1             -j hecatomb.out/processing/stats/p01/A13-256-117-06_ACTGAT.s1.stats.json -h hecatomb.out/processing/stats/p01/A13-256-117-06_ACTGAT.s1.stats.html             --qualified_quality_phred 15             --length_required 90             --detect_adapter_for_pe             --cut_tail --cut_tail_window_size 25 --cut_tail_mean_quality 15             --dedup --dup_calc_accuracy 4             --trim_poly_x             --thread 16 2> hecatomb.out/stderr/fastp_preprocessing.A13-256-117-06_ACTGAT.log
    rm hecatomb.out/stderr/fastp_preprocessing.A13-256-117-06_ACTGAT.log

Submitted job 49 with external jobid 'Submitted batch job 2287005'.

[Mon Mar 6 13:20:34 2023] rule fastp_preprocessing: input: /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-253-140-06_GTCCGC_R1.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-253-140-06_GTCCGC_R2.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/contaminants/vector_contaminants.fa output: hecatomb.out/processing/temp/p01/A13-253-140-06_GTCCGC_R1.s1.out.fastq, hecatomb.out/processing/temp/p01/A13-253-140-06_GTCCGC_R2.s1.out.fastq, hecatomb.out/processing/stats/p01/A13-253-140-06_GTCCGC.s1.stats.json, hecatomb.out/processing/stats/p01/A13-253-140-06_GTCCGC.s1.stats.html log: hecatomb.out/stderr/fastp_preprocessing.A13-253-140-06_GTCCGC.log jobid: 31 benchmark: hecatomb.out/benchmarks/fastp_preprocessing.A13-253-140-06_GTCCGC.txt reason: Missing output files: hecatomb.out/processing/temp/p01/A13-253-140-06_GTCCGC_R2.s1.out.fastq, hecatomb.out/processing/temp/p01/A13-253-140-06_GTCCGC_R1.s1.out.fastq wildcards: sample=A13-253-140-06_GTCCGC threads: 16 resources: mem_mb=32000, mem_mib=30518, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    fastp -i /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-253-140-06_GTCCGC_R1.fastq.gz -I /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-253-140-06_GTCCGC_R2.fastq.gz -o hecatomb.out/processing/temp/p01/A13-253-140-06_GTCCGC_R1.s1.out.fastq -O hecatomb.out/processing/temp/p01/A13-253-140-06_GTCCGC_R2.s1.out.fastq             -z 1             -j hecatomb.out/processing/stats/p01/A13-253-140-06_GTCCGC.s1.stats.json -h hecatomb.out/processing/stats/p01/A13-253-140-06_GTCCGC.s1.stats.html             --qualified_quality_phred 15             --length_required 90             --detect_adapter_for_pe             --cut_tail --cut_tail_window_size 25 --cut_tail_mean_quality 15             --dedup --dup_calc_accuracy 4             --trim_poly_x             --thread 16 2> hecatomb.out/stderr/fastp_preprocessing.A13-253-140-06_GTCCGC.log
    rm hecatomb.out/stderr/fastp_preprocessing.A13-253-140-06_GTCCGC.log

Submitted job 31 with external jobid 'Submitted batch job 2287006'.

[Mon Mar 6 13:20:34 2023] rule create_host_index: input: /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/host/human/masked_ref.fa.gz output: /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/host/human/masked_ref.fa.gz.idx log: hecatomb.out/stderr/create_host_index.log jobid: 7 benchmark: hecatomb.out/benchmarks/create_host_index.txt reason: Missing output files: /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/host/human/masked_ref.fa.gz.idx threads: 8 resources: mem_mb=16000, mem_mib=15259, disk_mb=1939, disk_mib=1850, tmpdir=, time=1440

    minimap2 -t 8 -d /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/host/human/masked_ref.fa.gz.idx <(cat /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/host/human/masked_ref.fa.gz) 2> hecatomb.out/stderr/create_host_index.log
    rm hecatomb.out/stderr/create_host_index.log

Submitted job 7 with external jobid 'Submitted batch job 2287007'.

[Mon Mar 6 13:20:34 2023] rule fastp_preprocessing: input: /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-115-06_GTTTCG_R1.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-115-06_GTTTCG_R2.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/contaminants/vector_contaminants.fa output: hecatomb.out/processing/temp/p01/A13-256-115-06_GTTTCG_R1.s1.out.fastq, hecatomb.out/processing/temp/p01/A13-256-115-06_GTTTCG_R2.s1.out.fastq, hecatomb.out/processing/stats/p01/A13-256-115-06_GTTTCG.s1.stats.json, hecatomb.out/processing/stats/p01/A13-256-115-06_GTTTCG.s1.stats.html log: hecatomb.out/stderr/fastp_preprocessing.A13-256-115-06_GTTTCG.log jobid: 55 benchmark: hecatomb.out/benchmarks/fastp_preprocessing.A13-256-115-06_GTTTCG.txt reason: Missing output files: hecatomb.out/processing/temp/p01/A13-256-115-06_GTTTCG_R1.s1.out.fastq, hecatomb.out/processing/temp/p01/A13-256-115-06_GTTTCG_R2.s1.out.fastq wildcards: sample=A13-256-115-06_GTTTCG threads: 16 resources: mem_mb=32000, mem_mib=30518, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    fastp -i /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-115-06_GTTTCG_R1.fastq.gz -I /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-115-06_GTTTCG_R2.fastq.gz -o hecatomb.out/processing/temp/p01/A13-256-115-06_GTTTCG_R1.s1.out.fastq -O hecatomb.out/processing/temp/p01/A13-256-115-06_GTTTCG_R2.s1.out.fastq             -z 1             -j hecatomb.out/processing/stats/p01/A13-256-115-06_GTTTCG.s1.stats.json -h hecatomb.out/processing/stats/p01/A13-256-115-06_GTTTCG.s1.stats.html             --qualified_quality_phred 15             --length_required 90             --detect_adapter_for_pe             --cut_tail --cut_tail_window_size 25 --cut_tail_mean_quality 15             --dedup --dup_calc_accuracy 4             --trim_poly_x             --thread 16 2> hecatomb.out/stderr/fastp_preprocessing.A13-256-115-06_GTTTCG.log
    rm hecatomb.out/stderr/fastp_preprocessing.A13-256-115-06_GTTTCG.log

Submitted job 55 with external jobid 'Submitted batch job 2287008'. Invalid jobs list: Submitted [Mon Mar 6 13:20:44 2023] Error in rule fastp_preprocessing: jobid: 43 input: /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-258-124-06_CGTACG_R1.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-258-124-06_CGTACG_R2.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/contaminants/vector_contaminants.fa output: hecatomb.out/processing/temp/p01/A13-258-124-06_CGTACG_R1.s1.out.fastq, hecatomb.out/processing/temp/p01/A13-258-124-06_CGTACG_R2.s1.out.fastq, hecatomb.out/processing/stats/p01/A13-258-124-06_CGTACG.s1.stats.json, hecatomb.out/processing/stats/p01/A13-258-124-06_CGTACG.s1.stats.html log: hecatomb.out/stderr/fastp_preprocessing.A13-258-124-06CGTACG.log (check log file(s) for error details) conda-env: /lustre/project/taw/share/conda-envs/hecatomb/snakemake/conda/4fabc0d9baac0a21fd5dfed339929234 shell:

    fastp -i /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-258-124-06_CGTACG_R1.fastq.gz -I /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-258-124-06_CGTACG_R2.fastq.gz -o hecatomb.out/processing/temp/p01/A13-258-124-06_CGTACG_R1.s1.out.fastq -O hecatomb.out/processing/temp/p01/A13-258-124-06_CGTACG_R2.s1.out.fastq             -z 1             -j hecatomb.out/processing/stats/p01/A13-258-124-06_CGTACG.s1.stats.json -h hecatomb.out/processing/stats/p01/A13-258-124-06_CGTACG.s1.stats.html             --qualified_quality_phred 15             --length_required 90             --detect_adapter_for_pe             --cut_tail --cut_tail_window_size 25 --cut_tail_mean_quality 15             --dedup --dup_calc_accuracy 4             --trim_poly_x             --thread 16 2> hecatomb.out/stderr/fastp_preprocessing.A13-258-124-06_CGTACG.log
    rm hecatomb.out/stderr/fastp_preprocessing.A13-258-124-06_CGTACG.log

    (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!)
cluster_jobid: Submitted batch job 2287003

Logfile hecatomb.out/stderr/fastp_preprocessing.A13-258-124-06_CGTACG.log not found.

Error executing rule fastp_preprocessing on cluster (jobid: 43, external: Submitted batch job 2287003, jobscript: /lustre/project/taw/share/conda-envs/.snakemake/tmp.skvkwg2w/snakejob.fastp_preprocessing.43.sh). For error details see the cluster log and the log files of the involved rule(s). Trying to restart job 43. Select jobs to execute...

[Mon Mar 6 13:20:44 2023] rule fastp_preprocessing: input: /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-258-124-06_CGTACG_R1.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-258-124-06_CGTACG_R2.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/contaminants/vector_contaminants.fa output: hecatomb.out/processing/temp/p01/A13-258-124-06_CGTACG_R1.s1.out.fastq, hecatomb.out/processing/temp/p01/A13-258-124-06_CGTACG_R2.s1.out.fastq, hecatomb.out/processing/stats/p01/A13-258-124-06_CGTACG.s1.stats.json, hecatomb.out/processing/stats/p01/A13-258-124-06_CGTACG.s1.stats.html log: hecatomb.out/stderr/fastp_preprocessing.A13-258-124-06_CGTACG.log jobid: 43 benchmark: hecatomb.out/benchmarks/fastp_preprocessing.A13-258-124-06_CGTACG.txt reason: Missing output files: hecatomb.out/processing/temp/p01/A13-258-124-06_CGTACG_R2.s1.out.fastq, hecatomb.out/processing/temp/p01/A13-258-124-06_CGTACG_R1.s1.out.fastq wildcards: sample=A13-258-124-06_CGTACG threads: 16 resources: mem_mb=32000, mem_mib=30518, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    fastp -i /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-258-124-06_CGTACG_R1.fastq.gz -I /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-258-124-06_CGTACG_R2.fastq.gz -o hecatomb.out/processing/temp/p01/A13-258-124-06_CGTACG_R1.s1.out.fastq -O hecatomb.out/processing/temp/p01/A13-258-124-06_CGTACG_R2.s1.out.fastq             -z 1             -j hecatomb.out/processing/stats/p01/A13-258-124-06_CGTACG.s1.stats.json -h hecatomb.out/processing/stats/p01/A13-258-124-06_CGTACG.s1.stats.html             --qualified_quality_phred 15             --length_required 90             --detect_adapter_for_pe             --cut_tail --cut_tail_window_size 25 --cut_tail_mean_quality 15             --dedup --dup_calc_accuracy 4             --trim_poly_x             --thread 16 2> hecatomb.out/stderr/fastp_preprocessing.A13-258-124-06_CGTACG.log
    rm hecatomb.out/stderr/fastp_preprocessing.A13-258-124-06_CGTACG.log

Submitted job 43 with external jobid 'Submitted batch job 2287009'. Invalid jobs list: Submitted [Mon Mar 6 13:20:44 2023] Error in rule dumpSamplesTsv: jobid: 252 output: hecatomb.out/results/hecatomb.samples.tsv cluster_jobid: Submitted batch job 2287004

Error executing rule dumpSamplesTsv on cluster (jobid: 252, external: Submitted batch job 2287004, jobscript: /lustre/project/taw/share/conda-envs/.snakemake/tmp.skvkwg2w/snakejob.dumpSamplesTsv.252.sh). For error details see the cluster log and the log files of the involved rule(s). Trying to restart job 252. Select jobs to execute...

[Mon Mar 6 13:20:44 2023] rule dumpSamplesTsv: output: hecatomb.out/results/hecatomb.samples.tsv jobid: 252 reason: Missing output files: hecatomb.out/results/hecatomb.samples.tsv resources: mem_mb=2000, mem_mib=1908, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

Submitted job 252 with external jobid 'Submitted batch job 2287010'. Invalid jobs list: Submitted [Mon Mar 6 13:20:45 2023] Error in rule fastp_preprocessing: jobid: 49 input: /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-117-06_ACTGAT_R1.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-117-06_ACTGAT_R2.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/contaminants/vector_contaminants.fa output: hecatomb.out/processing/temp/p01/A13-256-117-06_ACTGAT_R1.s1.out.fastq, hecatomb.out/processing/temp/p01/A13-256-117-06_ACTGAT_R2.s1.out.fastq, hecatomb.out/processing/stats/p01/A13-256-117-06_ACTGAT.s1.stats.json, hecatomb.out/processing/stats/p01/A13-256-117-06_ACTGAT.s1.stats.html log: hecatomb.out/stderr/fastp_preprocessing.A13-256-117-06ACTGAT.log (check log file(s) for error details) conda-env: /lustre/project/taw/share/conda-envs/hecatomb/snakemake/conda/4fabc0d9baac0a21fd5dfed339929234 shell:

    fastp -i /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-117-06_ACTGAT_R1.fastq.gz -I /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-117-06_ACTGAT_R2.fastq.gz -o hecatomb.out/processing/temp/p01/A13-256-117-06_ACTGAT_R1.s1.out.fastq -O hecatomb.out/processing/temp/p01/A13-256-117-06_ACTGAT_R2.s1.out.fastq             -z 1             -j hecatomb.out/processing/stats/p01/A13-256-117-06_ACTGAT.s1.stats.json -h hecatomb.out/processing/stats/p01/A13-256-117-06_ACTGAT.s1.stats.html             --qualified_quality_phred 15             --length_required 90             --detect_adapter_for_pe             --cut_tail --cut_tail_window_size 25 --cut_tail_mean_quality 15             --dedup --dup_calc_accuracy 4             --trim_poly_x             --thread 16 2> hecatomb.out/stderr/fastp_preprocessing.A13-256-117-06_ACTGAT.log
    rm hecatomb.out/stderr/fastp_preprocessing.A13-256-117-06_ACTGAT.log

    (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!)
cluster_jobid: Submitted batch job 2287005

Logfile hecatomb.out/stderr/fastp_preprocessing.A13-256-117-06_ACTGAT.log not found.

Error executing rule fastp_preprocessing on cluster (jobid: 49, external: Submitted batch job 2287005, jobscript: /lustre/project/taw/share/conda-envs/.snakemake/tmp.skvkwg2w/snakejob.fastp_preprocessing.49.sh). For error details see the cluster log and the log files of the involved rule(s). Trying to restart job 49. Select jobs to execute...

[Mon Mar 6 13:20:45 2023] rule fastp_preprocessing: input: /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-117-06_ACTGAT_R1.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-117-06_ACTGAT_R2.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/contaminants/vector_contaminants.fa output: hecatomb.out/processing/temp/p01/A13-256-117-06_ACTGAT_R1.s1.out.fastq, hecatomb.out/processing/temp/p01/A13-256-117-06_ACTGAT_R2.s1.out.fastq, hecatomb.out/processing/stats/p01/A13-256-117-06_ACTGAT.s1.stats.json, hecatomb.out/processing/stats/p01/A13-256-117-06_ACTGAT.s1.stats.html log: hecatomb.out/stderr/fastp_preprocessing.A13-256-117-06_ACTGAT.log jobid: 49 benchmark: hecatomb.out/benchmarks/fastp_preprocessing.A13-256-117-06_ACTGAT.txt reason: Missing output files: hecatomb.out/processing/temp/p01/A13-256-117-06_ACTGAT_R2.s1.out.fastq, hecatomb.out/processing/temp/p01/A13-256-117-06_ACTGAT_R1.s1.out.fastq wildcards: sample=A13-256-117-06_ACTGAT threads: 16 resources: mem_mb=32000, mem_mib=30518, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    fastp -i /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-117-06_ACTGAT_R1.fastq.gz -I /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-117-06_ACTGAT_R2.fastq.gz -o hecatomb.out/processing/temp/p01/A13-256-117-06_ACTGAT_R1.s1.out.fastq -O hecatomb.out/processing/temp/p01/A13-256-117-06_ACTGAT_R2.s1.out.fastq             -z 1             -j hecatomb.out/processing/stats/p01/A13-256-117-06_ACTGAT.s1.stats.json -h hecatomb.out/processing/stats/p01/A13-256-117-06_ACTGAT.s1.stats.html             --qualified_quality_phred 15             --length_required 90             --detect_adapter_for_pe             --cut_tail --cut_tail_window_size 25 --cut_tail_mean_quality 15             --dedup --dup_calc_accuracy 4             --trim_poly_x             --thread 16 2> hecatomb.out/stderr/fastp_preprocessing.A13-256-117-06_ACTGAT.log
    rm hecatomb.out/stderr/fastp_preprocessing.A13-256-117-06_ACTGAT.log

Submitted job 49 with external jobid 'Submitted batch job 2287011'. Invalid jobs list: Submitted [Mon Mar 6 13:20:45 2023] Error in rule fastp_preprocessing: jobid: 31 input: /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-253-140-06_GTCCGC_R1.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-253-140-06_GTCCGC_R2.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/contaminants/vector_contaminants.fa output: hecatomb.out/processing/temp/p01/A13-253-140-06_GTCCGC_R1.s1.out.fastq, hecatomb.out/processing/temp/p01/A13-253-140-06_GTCCGC_R2.s1.out.fastq, hecatomb.out/processing/stats/p01/A13-253-140-06_GTCCGC.s1.stats.json, hecatomb.out/processing/stats/p01/A13-253-140-06_GTCCGC.s1.stats.html log: hecatomb.out/stderr/fastp_preprocessing.A13-253-140-06GTCCGC.log (check log file(s) for error details) conda-env: /lustre/project/taw/share/conda-envs/hecatomb/snakemake/conda/4fabc0d9baac0a21fd5dfed339929234 shell:

    fastp -i /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-253-140-06_GTCCGC_R1.fastq.gz -I /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-253-140-06_GTCCGC_R2.fastq.gz -o hecatomb.out/processing/temp/p01/A13-253-140-06_GTCCGC_R1.s1.out.fastq -O hecatomb.out/processing/temp/p01/A13-253-140-06_GTCCGC_R2.s1.out.fastq             -z 1             -j hecatomb.out/processing/stats/p01/A13-253-140-06_GTCCGC.s1.stats.json -h hecatomb.out/processing/stats/p01/A13-253-140-06_GTCCGC.s1.stats.html             --qualified_quality_phred 15             --length_required 90             --detect_adapter_for_pe             --cut_tail --cut_tail_window_size 25 --cut_tail_mean_quality 15             --dedup --dup_calc_accuracy 4             --trim_poly_x             --thread 16 2> hecatomb.out/stderr/fastp_preprocessing.A13-253-140-06_GTCCGC.log
    rm hecatomb.out/stderr/fastp_preprocessing.A13-253-140-06_GTCCGC.log

    (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!)
cluster_jobid: Submitted batch job 2287006

Logfile hecatomb.out/stderr/fastp_preprocessing.A13-253-140-06_GTCCGC.log not found.

Error executing rule fastp_preprocessing on cluster (jobid: 31, external: Submitted batch job 2287006, jobscript: /lustre/project/taw/share/conda-envs/.snakemake/tmp.skvkwg2w/snakejob.fastp_preprocessing.31.sh). For error details see the cluster log and the log files of the involved rule(s). Trying to restart job 31. Select jobs to execute...

[Mon Mar 6 13:20:45 2023] rule fastp_preprocessing: input: /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-253-140-06_GTCCGC_R1.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-253-140-06_GTCCGC_R2.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/contaminants/vector_contaminants.fa output: hecatomb.out/processing/temp/p01/A13-253-140-06_GTCCGC_R1.s1.out.fastq, hecatomb.out/processing/temp/p01/A13-253-140-06_GTCCGC_R2.s1.out.fastq, hecatomb.out/processing/stats/p01/A13-253-140-06_GTCCGC.s1.stats.json, hecatomb.out/processing/stats/p01/A13-253-140-06_GTCCGC.s1.stats.html log: hecatomb.out/stderr/fastp_preprocessing.A13-253-140-06_GTCCGC.log jobid: 31 benchmark: hecatomb.out/benchmarks/fastp_preprocessing.A13-253-140-06_GTCCGC.txt reason: Missing output files: hecatomb.out/processing/temp/p01/A13-253-140-06_GTCCGC_R2.s1.out.fastq, hecatomb.out/processing/temp/p01/A13-253-140-06_GTCCGC_R1.s1.out.fastq wildcards: sample=A13-253-140-06_GTCCGC threads: 16 resources: mem_mb=32000, mem_mib=30518, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    fastp -i /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-253-140-06_GTCCGC_R1.fastq.gz -I /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-253-140-06_GTCCGC_R2.fastq.gz -o hecatomb.out/processing/temp/p01/A13-253-140-06_GTCCGC_R1.s1.out.fastq -O hecatomb.out/processing/temp/p01/A13-253-140-06_GTCCGC_R2.s1.out.fastq             -z 1             -j hecatomb.out/processing/stats/p01/A13-253-140-06_GTCCGC.s1.stats.json -h hecatomb.out/processing/stats/p01/A13-253-140-06_GTCCGC.s1.stats.html             --qualified_quality_phred 15             --length_required 90             --detect_adapter_for_pe             --cut_tail --cut_tail_window_size 25 --cut_tail_mean_quality 15             --dedup --dup_calc_accuracy 4             --trim_poly_x             --thread 16 2> hecatomb.out/stderr/fastp_preprocessing.A13-253-140-06_GTCCGC.log
    rm hecatomb.out/stderr/fastp_preprocessing.A13-253-140-06_GTCCGC.log

Submitted job 31 with external jobid 'Submitted batch job 2287012'. Invalid jobs list: Submitted [Mon Mar 6 13:20:45 2023] Error in rule create_host_index: jobid: 7 input: /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/host/human/masked_ref.fa.gz output: /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/host/human/masked_ref.fa.gz.idx log: hecatomb.out/stderr/create_hostindex.log (check log file(s) for error details) conda-env: /lustre/project/taw/share/conda-envs/hecatomb/snakemake/conda/4ea269956b660de957aed37e1acd2903 shell:

    minimap2 -t 8 -d /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/host/human/masked_ref.fa.gz.idx <(cat /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/host/human/masked_ref.fa.gz) 2> hecatomb.out/stderr/create_host_index.log
    rm hecatomb.out/stderr/create_host_index.log

    (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!)
cluster_jobid: Submitted batch job 2287007

Logfile hecatomb.out/stderr/create_host_index.log not found.

Error executing rule create_host_index on cluster (jobid: 7, external: Submitted batch job 2287007, jobscript: /lustre/project/taw/share/conda-envs/.snakemake/tmp.skvkwg2w/snakejob.create_host_index.7.sh). For error details see the cluster log and the log files of the involved rule(s). Trying to restart job 7. Select jobs to execute...

[Mon Mar 6 13:20:45 2023] rule create_host_index: input: /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/host/human/masked_ref.fa.gz output: /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/host/human/masked_ref.fa.gz.idx log: hecatomb.out/stderr/create_host_index.log jobid: 7 benchmark: hecatomb.out/benchmarks/create_host_index.txt reason: Missing output files: /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/host/human/masked_ref.fa.gz.idx threads: 8 resources: mem_mb=16000, mem_mib=15259, disk_mb=1939, disk_mib=1850, tmpdir=, time=1440

    minimap2 -t 8 -d /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/host/human/masked_ref.fa.gz.idx <(cat /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/host/human/masked_ref.fa.gz) 2> hecatomb.out/stderr/create_host_index.log
    rm hecatomb.out/stderr/create_host_index.log

Submitted job 7 with external jobid 'Submitted batch job 2287013'. Invalid jobs list: Submitted [Mon Mar 6 13:20:45 2023] Error in rule fastp_preprocessing: jobid: 55 input: /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-115-06_GTTTCG_R1.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-115-06_GTTTCG_R2.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/contaminants/vector_contaminants.fa output: hecatomb.out/processing/temp/p01/A13-256-115-06_GTTTCG_R1.s1.out.fastq, hecatomb.out/processing/temp/p01/A13-256-115-06_GTTTCG_R2.s1.out.fastq, hecatomb.out/processing/stats/p01/A13-256-115-06_GTTTCG.s1.stats.json, hecatomb.out/processing/stats/p01/A13-256-115-06_GTTTCG.s1.stats.html log: hecatomb.out/stderr/fastp_preprocessing.A13-256-115-06GTTTCG.log (check log file(s) for error details) conda-env: /lustre/project/taw/share/conda-envs/hecatomb/snakemake/conda/4fabc0d9baac0a21fd5dfed339929234 shell:

    fastp -i /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-115-06_GTTTCG_R1.fastq.gz -I /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-115-06_GTTTCG_R2.fastq.gz -o hecatomb.out/processing/temp/p01/A13-256-115-06_GTTTCG_R1.s1.out.fastq -O hecatomb.out/processing/temp/p01/A13-256-115-06_GTTTCG_R2.s1.out.fastq             -z 1             -j hecatomb.out/processing/stats/p01/A13-256-115-06_GTTTCG.s1.stats.json -h hecatomb.out/processing/stats/p01/A13-256-115-06_GTTTCG.s1.stats.html             --qualified_quality_phred 15             --length_required 90             --detect_adapter_for_pe             --cut_tail --cut_tail_window_size 25 --cut_tail_mean_quality 15             --dedup --dup_calc_accuracy 4             --trim_poly_x             --thread 16 2> hecatomb.out/stderr/fastp_preprocessing.A13-256-115-06_GTTTCG.log
    rm hecatomb.out/stderr/fastp_preprocessing.A13-256-115-06_GTTTCG.log

    (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!)
cluster_jobid: Submitted batch job 2287008

Logfile hecatomb.out/stderr/fastp_preprocessing.A13-256-115-06_GTTTCG.log not found.

Error executing rule fastp_preprocessing on cluster (jobid: 55, external: Submitted batch job 2287008, jobscript: /lustre/project/taw/share/conda-envs/.snakemake/tmp.skvkwg2w/snakejob.fastp_preprocessing.55.sh). For error details see the cluster log and the log files of the involved rule(s). Trying to restart job 55. Select jobs to execute...

[Mon Mar 6 13:20:45 2023] rule fastp_preprocessing: input: /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-115-06_GTTTCG_R1.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-115-06_GTTTCG_R2.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/contaminants/vector_contaminants.fa output: hecatomb.out/processing/temp/p01/A13-256-115-06_GTTTCG_R1.s1.out.fastq, hecatomb.out/processing/temp/p01/A13-256-115-06_GTTTCG_R2.s1.out.fastq, hecatomb.out/processing/stats/p01/A13-256-115-06_GTTTCG.s1.stats.json, hecatomb.out/processing/stats/p01/A13-256-115-06_GTTTCG.s1.stats.html log: hecatomb.out/stderr/fastp_preprocessing.A13-256-115-06_GTTTCG.log jobid: 55 benchmark: hecatomb.out/benchmarks/fastp_preprocessing.A13-256-115-06_GTTTCG.txt reason: Missing output files: hecatomb.out/processing/temp/p01/A13-256-115-06_GTTTCG_R1.s1.out.fastq, hecatomb.out/processing/temp/p01/A13-256-115-06_GTTTCG_R2.s1.out.fastq wildcards: sample=A13-256-115-06_GTTTCG threads: 16 resources: mem_mb=32000, mem_mib=30518, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    fastp -i /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-115-06_GTTTCG_R1.fastq.gz -I /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-115-06_GTTTCG_R2.fastq.gz -o hecatomb.out/processing/temp/p01/A13-256-115-06_GTTTCG_R1.s1.out.fastq -O hecatomb.out/processing/temp/p01/A13-256-115-06_GTTTCG_R2.s1.out.fastq             -z 1             -j hecatomb.out/processing/stats/p01/A13-256-115-06_GTTTCG.s1.stats.json -h hecatomb.out/processing/stats/p01/A13-256-115-06_GTTTCG.s1.stats.html             --qualified_quality_phred 15             --length_required 90             --detect_adapter_for_pe             --cut_tail --cut_tail_window_size 25 --cut_tail_mean_quality 15             --dedup --dup_calc_accuracy 4             --trim_poly_x             --thread 16 2> hecatomb.out/stderr/fastp_preprocessing.A13-256-115-06_GTTTCG.log
    rm hecatomb.out/stderr/fastp_preprocessing.A13-256-115-06_GTTTCG.log

Submitted job 55 with external jobid 'Submitted batch job 2287014'. Invalid jobs list: Submitted [Mon Mar 6 13:20:55 2023] Error in rule fastp_preprocessing: jobid: 43 input: /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-258-124-06_CGTACG_R1.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-258-124-06_CGTACG_R2.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/contaminants/vector_contaminants.fa output: hecatomb.out/processing/temp/p01/A13-258-124-06_CGTACG_R1.s1.out.fastq, hecatomb.out/processing/temp/p01/A13-258-124-06_CGTACG_R2.s1.out.fastq, hecatomb.out/processing/stats/p01/A13-258-124-06_CGTACG.s1.stats.json, hecatomb.out/processing/stats/p01/A13-258-124-06_CGTACG.s1.stats.html log: hecatomb.out/stderr/fastp_preprocessing.A13-258-124-06CGTACG.log (check log file(s) for error details) conda-env: /lustre/project/taw/share/conda-envs/hecatomb/snakemake/conda/4fabc0d9baac0a21fd5dfed339929234 shell:

    fastp -i /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-258-124-06_CGTACG_R1.fastq.gz -I /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-258-124-06_CGTACG_R2.fastq.gz -o hecatomb.out/processing/temp/p01/A13-258-124-06_CGTACG_R1.s1.out.fastq -O hecatomb.out/processing/temp/p01/A13-258-124-06_CGTACG_R2.s1.out.fastq             -z 1             -j hecatomb.out/processing/stats/p01/A13-258-124-06_CGTACG.s1.stats.json -h hecatomb.out/processing/stats/p01/A13-258-124-06_CGTACG.s1.stats.html             --qualified_quality_phred 15             --length_required 90             --detect_adapter_for_pe             --cut_tail --cut_tail_window_size 25 --cut_tail_mean_quality 15             --dedup --dup_calc_accuracy 4             --trim_poly_x             --thread 16 2> hecatomb.out/stderr/fastp_preprocessing.A13-258-124-06_CGTACG.log
    rm hecatomb.out/stderr/fastp_preprocessing.A13-258-124-06_CGTACG.log

    (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!)
cluster_jobid: Submitted batch job 2287009

Logfile hecatomb.out/stderr/fastp_preprocessing.A13-258-124-06_CGTACG.log not found.

Error executing rule fastp_preprocessing on cluster (jobid: 43, external: Submitted batch job 2287009, jobscript: /lustre/project/taw/share/conda-envs/.snakemake/tmp.skvkwg2w/snakejob.fastp_preprocessing.43.sh). For error details see the cluster log and the log files of the involved rule(s). Invalid jobs list: Submitted [Mon Mar 6 13:20:55 2023] Error in rule dumpSamplesTsv: jobid: 252 output: hecatomb.out/results/hecatomb.samples.tsv cluster_jobid: Submitted batch job 2287010

Error executing rule dumpSamplesTsv on cluster (jobid: 252, external: Submitted batch job 2287010, jobscript: /lustre/project/taw/share/conda-envs/.snakemake/tmp.skvkwg2w/snakejob.dumpSamplesTsv.252.sh). For error details see the cluster log and the log files of the involved rule(s). Invalid jobs list: Submitted [Mon Mar 6 13:20:56 2023] Error in rule fastp_preprocessing: jobid: 49 input: /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-117-06_ACTGAT_R1.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-117-06_ACTGAT_R2.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/contaminants/vector_contaminants.fa output: hecatomb.out/processing/temp/p01/A13-256-117-06_ACTGAT_R1.s1.out.fastq, hecatomb.out/processing/temp/p01/A13-256-117-06_ACTGAT_R2.s1.out.fastq, hecatomb.out/processing/stats/p01/A13-256-117-06_ACTGAT.s1.stats.json, hecatomb.out/processing/stats/p01/A13-256-117-06_ACTGAT.s1.stats.html log: hecatomb.out/stderr/fastp_preprocessing.A13-256-117-06ACTGAT.log (check log file(s) for error details) conda-env: /lustre/project/taw/share/conda-envs/hecatomb/snakemake/conda/4fabc0d9baac0a21fd5dfed339929234 shell:

    fastp -i /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-117-06_ACTGAT_R1.fastq.gz -I /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-117-06_ACTGAT_R2.fastq.gz -o hecatomb.out/processing/temp/p01/A13-256-117-06_ACTGAT_R1.s1.out.fastq -O hecatomb.out/processing/temp/p01/A13-256-117-06_ACTGAT_R2.s1.out.fastq             -z 1             -j hecatomb.out/processing/stats/p01/A13-256-117-06_ACTGAT.s1.stats.json -h hecatomb.out/processing/stats/p01/A13-256-117-06_ACTGAT.s1.stats.html             --qualified_quality_phred 15             --length_required 90             --detect_adapter_for_pe             --cut_tail --cut_tail_window_size 25 --cut_tail_mean_quality 15             --dedup --dup_calc_accuracy 4             --trim_poly_x             --thread 16 2> hecatomb.out/stderr/fastp_preprocessing.A13-256-117-06_ACTGAT.log
    rm hecatomb.out/stderr/fastp_preprocessing.A13-256-117-06_ACTGAT.log

    (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!)
cluster_jobid: Submitted batch job 2287011

Logfile hecatomb.out/stderr/fastp_preprocessing.A13-256-117-06_ACTGAT.log not found.

Error executing rule fastp_preprocessing on cluster (jobid: 49, external: Submitted batch job 2287011, jobscript: /lustre/project/taw/share/conda-envs/.snakemake/tmp.skvkwg2w/snakejob.fastp_preprocessing.49.sh). For error details see the cluster log and the log files of the involved rule(s). Invalid jobs list: Submitted [Mon Mar 6 13:20:56 2023] Error in rule fastp_preprocessing: jobid: 31 input: /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-253-140-06_GTCCGC_R1.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-253-140-06_GTCCGC_R2.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/contaminants/vector_contaminants.fa output: hecatomb.out/processing/temp/p01/A13-253-140-06_GTCCGC_R1.s1.out.fastq, hecatomb.out/processing/temp/p01/A13-253-140-06_GTCCGC_R2.s1.out.fastq, hecatomb.out/processing/stats/p01/A13-253-140-06_GTCCGC.s1.stats.json, hecatomb.out/processing/stats/p01/A13-253-140-06_GTCCGC.s1.stats.html log: hecatomb.out/stderr/fastp_preprocessing.A13-253-140-06GTCCGC.log (check log file(s) for error details) conda-env: /lustre/project/taw/share/conda-envs/hecatomb/snakemake/conda/4fabc0d9baac0a21fd5dfed339929234 shell:

    fastp -i /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-253-140-06_GTCCGC_R1.fastq.gz -I /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-253-140-06_GTCCGC_R2.fastq.gz -o hecatomb.out/processing/temp/p01/A13-253-140-06_GTCCGC_R1.s1.out.fastq -O hecatomb.out/processing/temp/p01/A13-253-140-06_GTCCGC_R2.s1.out.fastq             -z 1             -j hecatomb.out/processing/stats/p01/A13-253-140-06_GTCCGC.s1.stats.json -h hecatomb.out/processing/stats/p01/A13-253-140-06_GTCCGC.s1.stats.html             --qualified_quality_phred 15             --length_required 90             --detect_adapter_for_pe             --cut_tail --cut_tail_window_size 25 --cut_tail_mean_quality 15             --dedup --dup_calc_accuracy 4             --trim_poly_x             --thread 16 2> hecatomb.out/stderr/fastp_preprocessing.A13-253-140-06_GTCCGC.log
    rm hecatomb.out/stderr/fastp_preprocessing.A13-253-140-06_GTCCGC.log

    (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!)
cluster_jobid: Submitted batch job 2287012

Logfile hecatomb.out/stderr/fastp_preprocessing.A13-253-140-06_GTCCGC.log not found.

Error executing rule fastp_preprocessing on cluster (jobid: 31, external: Submitted batch job 2287012, jobscript: /lustre/project/taw/share/conda-envs/.snakemake/tmp.skvkwg2w/snakejob.fastp_preprocessing.31.sh). For error details see the cluster log and the log files of the involved rule(s). Invalid jobs list: Submitted [Mon Mar 6 13:20:56 2023] Error in rule create_host_index: jobid: 7 input: /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/host/human/masked_ref.fa.gz output: /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/host/human/masked_ref.fa.gz.idx log: hecatomb.out/stderr/create_hostindex.log (check log file(s) for error details) conda-env: /lustre/project/taw/share/conda-envs/hecatomb/snakemake/conda/4ea269956b660de957aed37e1acd2903 shell:

    minimap2 -t 8 -d /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/host/human/masked_ref.fa.gz.idx <(cat /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/host/human/masked_ref.fa.gz) 2> hecatomb.out/stderr/create_host_index.log
    rm hecatomb.out/stderr/create_host_index.log

    (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!)
cluster_jobid: Submitted batch job 2287013

Logfile hecatomb.out/stderr/create_host_index.log not found.

Error executing rule create_host_index on cluster (jobid: 7, external: Submitted batch job 2287013, jobscript: /lustre/project/taw/share/conda-envs/.snakemake/tmp.skvkwg2w/snakejob.create_host_index.7.sh). For error details see the cluster log and the log files of the involved rule(s). Invalid jobs list: Submitted [Mon Mar 6 13:20:56 2023] Error in rule fastp_preprocessing: jobid: 55 input: /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-115-06_GTTTCG_R1.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-115-06_GTTTCG_R2.fastq.gz, /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/contaminants/vector_contaminants.fa output: hecatomb.out/processing/temp/p01/A13-256-115-06_GTTTCG_R1.s1.out.fastq, hecatomb.out/processing/temp/p01/A13-256-115-06_GTTTCG_R2.s1.out.fastq, hecatomb.out/processing/stats/p01/A13-256-115-06_GTTTCG.s1.stats.json, hecatomb.out/processing/stats/p01/A13-256-115-06_GTTTCG.s1.stats.html log: hecatomb.out/stderr/fastp_preprocessing.A13-256-115-06GTTTCG.log (check log file(s) for error details) conda-env: /lustre/project/taw/share/conda-envs/hecatomb/snakemake/conda/4fabc0d9baac0a21fd5dfed339929234 shell:

    fastp -i /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-115-06_GTTTCG_R1.fastq.gz -I /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data/A13-256-115-06_GTTTCG_R2.fastq.gz -o hecatomb.out/processing/temp/p01/A13-256-115-06_GTTTCG_R1.s1.out.fastq -O hecatomb.out/processing/temp/p01/A13-256-115-06_GTTTCG_R2.s1.out.fastq             -z 1             -j hecatomb.out/processing/stats/p01/A13-256-115-06_GTTTCG.s1.stats.json -h hecatomb.out/processing/stats/p01/A13-256-115-06_GTTTCG.s1.stats.html             --qualified_quality_phred 15             --length_required 90             --detect_adapter_for_pe             --cut_tail --cut_tail_window_size 25 --cut_tail_mean_quality 15             --dedup --dup_calc_accuracy 4             --trim_poly_x             --thread 16 2> hecatomb.out/stderr/fastp_preprocessing.A13-256-115-06_GTTTCG.log
    rm hecatomb.out/stderr/fastp_preprocessing.A13-256-115-06_GTTTCG.log

    (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!)
cluster_jobid: Submitted batch job 2287014

Logfile hecatomb.out/stderr/fastp_preprocessing.A13-256-115-06_GTTTCG.log not found.

Error executing rule fastp_preprocessing on cluster (jobid: 55, external: Submitted batch job 2287014, jobscript: /lustre/project/taw/share/conda-envs/.snakemake/tmp.skvkwg2w/snakejob.fastp_preprocessing.55.sh). For error details see the cluster log and the log files of the involved rule(s). Exiting because a job execution failed. Look above for error message

FATAL: Hecatomb encountered an error.
       Dumping all error logs to "hecatomb.errorLogs.txt"Complete log: .snakemake/log/2023-03-06T132026.448612.snakemake.log

[2023:03:06 13:21:06] Error: Snakemake failed

beardymcjohnface commented 1 year ago

Progress! I think this is the same issue as #87, can you check your profile config.yaml file to make sure you've got --parsable for sbatch?

katievigil commented 1 year ago

2023-03-06T145158.281223.snakemake.log 2023-03-06T145440.340562.snakemake.log 2023-03-06T145809.129136.snakemake.log 2023-03-06T145820.167039.snakemake.log 2023-03-06T130924.336960.snakemake.log 2023-03-06T132026.448612.snakemake.log

Here are all my logs if this helps

katievigil commented 1 year ago

Here is what that file says:

#####################################################

| | __ | | __ _ | |

| ' \ / \/ _/ | __/ _ \| '_ | ' \

| | | | _/ (| (| | || () | | | | | | |_) |

|| ||_|__,|___/|| || ||./

#####################################################

For more information see: https://github.com/shandley/hecatomb and https://hecatomb.readthedocs.io/en/latest/

##################

Run Parameters

##################

Inputs and outputs (specify Reads here or on the command line, Output blank = use default)

Reads: Output:

Database installation location, leave blank = use hecatomb install location

Databases:

STICK TO YOUR SYSTEM'S CPU:RAM RATIO FOR THESE

BigJobMem: 64000 # Memory for MMSeqs in megabytes (e.g 64GB = 64000, recommend >= 64000) BigJobCpu: 24 # Threads for MMSeqs (recommend >= 16) BigJobTimeMin: 1440 # Max runtime in minutes for MMSeqs (this is only enforced by the Snakemake profile) MediumJobMem: 32000 # Memory for Megahit/Flye in megabytes (recommend >= 32000) MediumJobCpu: 16 # CPUs for Megahit/Flye in megabytes (recommend >= 16) SmallJobMem: 16000 # Memory for BBTools etc. in megabytes (recommend >= 16000) SmallJobCpu: 8 # CPUs for BBTools etc. (recommend >= 8)

Some jobs need more RAM; go over your CPU:RAM ratio if needed

MoreRamMem: 16000 # Memory for slightly RAM-hungry jobs in megabytes (recommend >= 16000) MoreRamCpu: 2 # CPUs for slightly RAM-hungry jobs (recommend >= 2)

############################

Optional Rule Parameters

############################

Preprocessing

QSCORE: 15 # Read quality trimming score (rule fastp_preprocessing in 01_preprocessing.smk) READ_MINLENGTH: 90 # Minimum read length during QC steps (rule fastp_preprocessing in 01_preprocessing.smk) CONTIG_MINLENGTH: 1000 # Read minimum length (rule contig_reformating_and_stats in 01_preprocessing.smk) CUTTAIL_WINDOW: 25 # Sliding window size for low qual read filter rule fastp_preprocessing in 01_preprocessing.smk) DEDUP_ACCURACY: 4 # Specify the level (1 ~ 6). The higher level means more memory usage and more running time, but lower risk of incorrect deduplication marking (rule fastp_preprocessing in 01_preprocessing.smk) COMPRESSION: 1 # Compression level for gzip output (1 ~ 9). 1 is fastest, 9 is smallest. Default is 1, based on assumption of large scratch space (rule fastp_preprocessing in 01_preprocessing.smk) ENTROPY: 0.5 # Read minimum entropy (rule remove_low_quality in 01_preprocessing.smk) ENTROPYWINDOW: 25 # entropy window for low qual read filter

CLUSTER READS TO SEQTABLE (MMSEQS EASY-LINCLUST)

-c = req coverage of target seq

--min-seq-id = req identity [0-1] of alignment

linclustParams: --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3

If the following LCA-calculated TaxIDs are encountered, defer to the top hit TaxID

0 = unclassified

1 = root

2 = bacteria root

10239 = virus root

131567 = cell org root

12429 = unclass virus

2759 = eukaryota root

12333 = unclass phage

taxIdIgnore: 0 1 2 10239 131567 12429 2759

###########################################

Canu settings (for longread assemblies)

###########################################

recommended correctedErrorRate is 0.16 to 0.12 (depending on coverage) for nanopore and

0.105 to 0.040 (depending on coverage) for pacbio (non-HiFi) - below is low-coverage nanopore

https://canu.readthedocs.io/en/latest/faq.html#what-parameters-can-i-tweak

canuSettings: correctedErrorRate=0.16 maxInputCoverage=10000 minInputCoverage=0 corOutCoverage=10000 corMhapSensitivity=high corMinCoverage=0 useGrid=False stopOnLowCoverage=False genomeSize=10M -nanopore

-pacbio

-pacbio-hifi

###################

MMSeqs settings

###################

ALIGNMENT FILTERING CUTOFFS

--min-length for AA should be equal or less than 1/3 of READ_MINLENGTH

--min-length for NT should be equal or less than READ_MINLENGTH

filtAAprimary: --min-length 30 -e 1e-3 filtAAsecondary: --min-length 30 -e 1e-5 filtNTprimary: --min-length 90 -e 1e-10 filtNTsecondary: --min-length 90 -e 1e-20

PERFORMANCE SETTINGS - SEE MMSEQS DOCUMENTATION FOR DETAILS

sensitive AA search

perfAA: --start-sens 1 --sens-steps 3 -s 7 --lca-mode 2 --shuffle 0

fast AA search

perfAAfast: -s 4.0 --lca-mode 2 --shuffle 0

sensitive NT search

perfNT: --start-sens 2 -s 7 --sens-steps 3

fast NT search

perfNTfast: -s 4.0

katievigil commented 1 year ago

OOPs I found the correct yam file: I added --parsable at the end of sbatch and it is running now!

cluster: mkdir -p logs/{rule}/ && sbatch --cpus-per-task={threads} --mem={resources.mem_mb} --time={resources.time} --job-name=smk-{rule} --output=logs/{rule}/{jobid}.out --error=logs/{rule}/{jobid}.err --parsable default-resources:

katievigil commented 1 year ago

Hi I am still getting an error with the slurm test:

(base) [kvigil@cypress01-123 ~]$ hecatomb test --profile slurm

██╗ ██╗███████╗ ██████╗ █████╗ ████████╗ ██████╗ ███╗ ███╗██████╗ ██║ ██║██╔════╝██╔════╝██╔══██╗╚══██╔══╝██╔═══██╗████╗ ████║██╔══██╗ ███████║█████╗ ██║ ███████║ ██║ ██║ ██║██╔████╔██║██████╔╝ ██╔══██║██╔══╝ ██║ ██╔══██║ ██║ ██║ ██║██║╚██╔╝██║██╔══██╗ ██║ ██║███████╗╚██████╗██║ ██║ ██║ ╚██████╔╝██║ ╚═╝ ██║██████╔╝ ╚═╝ ╚═╝╚══════╝ ╚═════╝╚═╝ ╚═╝ ╚═╝ ╚═════╝ ╚═╝ ╚═╝╚═════╝

Hecatomb version v1.1.0

[2023:03:09 11:54:15] Config file hecatomb.config.yaml already exists. Using existing config file. [2023:03:09 11:54:15] Writing runtime config file to hecatomb.config.yaml [2023:03:09 11:54:15] ------------------ [2023:03:09 11:54:15] | Runtime config | [2023:03:09 11:54:15] ------------------

BigJobCpu: 24 BigJobMem: 64000 BigJobTimeMin: 1440 COMPRESSION: 1 CONTIG_MINLENGTH: 1000 CUTTAIL_WINDOW: 25 DEDUP_ACCURACY: 4 Databases: null ENTROPY: 0.5 ENTROPYWINDOW: 25 Host: human MediumJobCpu: 16 MediumJobMem: 32000 MoreRamCpu: 2 MoreRamMem: 16000 Output: hecatomb.out Preprocess: paired QSCORE: 15 READ_MINLENGTH: 90 Reads: /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data Search: sensitive SmallJobCpu: 8 SmallJobMem: 16000 canuSettings: correctedErrorRate=0.16 maxInputCoverage=10000 minInputCoverage=0 corOutCoverage=10000 corMhapSensitivity=high corMinCoverage=0 useGrid=False stopOnLowCoverage=False genomeSize=10M -nanopore filtAAprimary: --min-length 30 -e 1e-3 filtAAsecondary: --min-length 30 -e 1e-5 filtNTprimary: --min-length 90 -e 1e-10 filtNTsecondary: --min-length 90 -e 1e-20 linclustParams: --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3 perfAA: --start-sens 1 --sens-steps 3 -s 7 --lca-mode 2 --shuffle 0 perfAAfast: -s 4.0 --lca-mode 2 --shuffle 0 perfNT: --start-sens 2 -s 7 --sens-steps 3 perfNTfast: -s 4.0 taxIdIgnore: 0 1 2 10239 131567 12429 2759

[2023:03:09 11:54:15] --------------------- [2023:03:09 11:54:15] | Snakemake command | [2023:03:09 11:54:15] ---------------------

snakemake -s /lustre/project/taw/share/conda-envs/hecatomb/bin/../snakemake/workflow/Hecatomb.smk --configfile hecatomb.config.yaml --use-conda --conda-frontend mamba --conda-prefix /lustre/project/taw/share/conda-envs/hecatomb/bin/../snakemake/conda --rerun-incomplete --printshellcmds --nolock --show-failed-logs --profile slurm Config file /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../config/config.yaml is extended by additional config specified via the command line. Config file /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../config/dbFiles.yaml is extended by additional config specified via the command line. Config file /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../config/immutable.yaml is extended by additional config specified via the command line. Building DAG of jobs... Your conda installation is not configured to use strict channel priorities. This is however crucial for having robust and correct environments (for details, see https://conda-forge.org/docs/user/tipsandtricks.html). Please consider to configure strict priorities by executing 'conda config --set channel_priority strict'. Using shell: /bin/bash Provided cluster nodes: 100 Job stats: job count min threads max threads

PRIMARY_AA_parsing 1 2 2 PRIMARY_AA_taxonomy_assignment 1 24 24 PRIMARY_NT_parsing 1 2 2 PRIMARY_NT_reformat 1 2 2 PRIMARY_NT_taxonomic_assignment 1 24 24 SECONDARY_AA_generate_output_table 1 2 2 SECONDARY_AA_parsing 1 2 2 SECONDARY_AA_refactor_finalize 1 2 2 SECONDARY_AA_taxonomy_assignment 1 24 24 SECONDARY_AA_tophit_lineage 1 2 2 SECONDARY_NT_convert 1 24 24 SECONDARY_NT_generate_output_table 1 2 2 SECONDARY_NT_summary 1 2 2 SECONDARY_NT_taxonomic_assignment 1 24 24 all 1 1 1 bam_index 1 2 2 calculate_gc 2 2 2 calculate_tet_freq 2 2 2 cluster_similar_sequences 10 24 24 combine_AA_NT 1 1 1 concatenate_contigs 1 1 1 concatentate_contig_count_tables 1 1 1 contig_krona_plot 1 1 1 contig_krona_text_format 1 1 1 contig_read_taxonomy 1 2 2 coverage_calculations 10 8 8 create_contig_count_table 10 1 1 create_individual_seqtables 10 24 24 krona_plot 1 1 1 krona_text_format 1 1 1 link_assembly 1 1 1 map_seq_table 1 16 16 merge_seq_table 1 1 1 mmseqs_contig_annotation 1 24 24 mmseqs_contig_annotation_summary 1 24 24 population_assembly 1 16 16 rescue_read_kmer_normalization 1 24 24 secondary_nt_calc_lca 1 24 24 secondary_nt_lca_table 1 1 1 seq_properties_table 2 2 2 tax_level_counts 1 2 2 unmapped_read_rescue_assembly 1 16 16 total 81 1 24

Select jobs to execute...

[Thu Mar 9 11:54:19 2023] rule cluster_similar_sequences: input: hecatomb.out/processing/temp/p04/A13-135-177-06_AGTTCC_R1.all.fastq output: hecatomb.out/processing/temp/p05/A13-135-177-06_AGTTCC_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-135-177-06_AGTTCC_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-135-177-06_AGTTCC_R1_all_seqs.fasta log: hecatomb.out/stderr/cluster_similar_sequences.A13-135-177-06_AGTTCC.log jobid: 10 benchmark: hecatomb.out/benchmarks/cluster_similar_sequences.A13-135-177-06_AGTTCC.txt reason: Missing output files: hecatomb.out/processing/temp/p05/A13-135-177-06_AGTTCC_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-135-177-06_AGTTCC_R1_rep_seq.fasta; Updated input files: hecatomb.out/processing/temp/p04/A13-135-177-06_AGTTCC_R1.all.fastq wildcards: sample=A13-135-177-06_AGTTCC threads: 24 resources: mem_mb=64000, mem_mib=61036, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    mmseqs easy-linclust hecatomb.out/processing/temp/p04/A13-135-177-06_AGTTCC_R1.all.fastq hecatomb.out/processing/temp/p05/A13-135-177-06_AGTTCC_R1 hecatomb.out/processing/temp/p05/A13-135-177-06_AGTTCC_TMP             --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3             --threads 24 &> hecatomb.out/stderr/cluster_similar_sequences.A13-135-177-06_AGTTCC.log
    rm hecatomb.out/stderr/cluster_similar_sequences.A13-135-177-06_AGTTCC.log

sbatch: error: Batch job submission failed: Requested node configuration is not available Error submitting jobscript (exit code 1):

[Thu Mar 9 11:54:19 2023] rule cluster_similar_sequences: input: hecatomb.out/processing/temp/p04/A13-252-114-06_CCGTCC_R1.all.fastq output: hecatomb.out/processing/temp/p05/A13-252-114-06_CCGTCC_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-252-114-06_CCGTCC_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-252-114-06_CCGTCC_R1_all_seqs.fasta log: hecatomb.out/stderr/cluster_similar_sequences.A13-252-114-06_CCGTCC.log jobid: 58 benchmark: hecatomb.out/benchmarks/cluster_similar_sequences.A13-252-114-06_CCGTCC.txt reason: Missing output files: hecatomb.out/processing/temp/p05/A13-252-114-06_CCGTCC_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-252-114-06_CCGTCC_R1_cluster.tsv; Updated input files: hecatomb.out/processing/temp/p04/A13-252-114-06_CCGTCC_R1.all.fastq wildcards: sample=A13-252-114-06_CCGTCC threads: 24 resources: mem_mb=64000, mem_mib=61036, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    mmseqs easy-linclust hecatomb.out/processing/temp/p04/A13-252-114-06_CCGTCC_R1.all.fastq hecatomb.out/processing/temp/p05/A13-252-114-06_CCGTCC_R1 hecatomb.out/processing/temp/p05/A13-252-114-06_CCGTCC_TMP             --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3             --threads 24 &> hecatomb.out/stderr/cluster_similar_sequences.A13-252-114-06_CCGTCC.log
    rm hecatomb.out/stderr/cluster_similar_sequences.A13-252-114-06_CCGTCC.log

sbatch: error: Batch job submission failed: Requested node configuration is not available Error submitting jobscript (exit code 1):

[Thu Mar 9 11:54:19 2023] rule cluster_similar_sequences: input: hecatomb.out/processing/temp/p04/A13-12-250-06_GGCTAC_R1.all.fastq output: hecatomb.out/processing/temp/p05/A13-12-250-06_GGCTAC_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-12-250-06_GGCTAC_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-12-250-06_GGCTAC_R1_all_seqs.fasta log: hecatomb.out/stderr/cluster_similar_sequences.A13-12-250-06_GGCTAC.log jobid: 34 benchmark: hecatomb.out/benchmarks/cluster_similar_sequences.A13-12-250-06_GGCTAC.txt reason: Missing output files: hecatomb.out/processing/temp/p05/A13-12-250-06_GGCTAC_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-12-250-06_GGCTAC_R1_rep_seq.fasta; Updated input files: hecatomb.out/processing/temp/p04/A13-12-250-06_GGCTAC_R1.all.fastq wildcards: sample=A13-12-250-06_GGCTAC threads: 24 resources: mem_mb=64000, mem_mib=61036, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    mmseqs easy-linclust hecatomb.out/processing/temp/p04/A13-12-250-06_GGCTAC_R1.all.fastq hecatomb.out/processing/temp/p05/A13-12-250-06_GGCTAC_R1 hecatomb.out/processing/temp/p05/A13-12-250-06_GGCTAC_TMP             --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3             --threads 24 &> hecatomb.out/stderr/cluster_similar_sequences.A13-12-250-06_GGCTAC.log
    rm hecatomb.out/stderr/cluster_similar_sequences.A13-12-250-06_GGCTAC.log

sbatch: error: Batch job submission failed: Requested node configuration is not available Error submitting jobscript (exit code 1):

[Thu Mar 9 11:54:19 2023] rule rescue_read_kmer_normalization: input: hecatomb.out/processing/assembly/unmapRescue_R1.fastq, hecatomb.out/processing/assembly/unmapRescue_R2.fastq, hecatomb.out/processing/assembly/unmapRescue.s.fastq output: hecatomb.out/processing/assembly/unmapRescueNorm_R1.fastq, hecatomb.out/processing/assembly/unmapRescueNorm_R2.fastq log: hecatomb.out/stderr/rescue_read_kmer_normalization.log jobid: 160 benchmark: hecatomb.out/benchmarks/rescue_read_kmer_normalization.txt reason: Missing output files: hecatomb.out/processing/assembly/unmapRescueNorm_R1.fastq, hecatomb.out/processing/assembly/unmapRescueNorm_R2.fastq; Updated input files: hecatomb.out/processing/assembly/unmapRescue_R1.fastq, hecatomb.out/processing/assembly/unmapRescue.s.fastq, hecatomb.out/processing/assembly/unmapRescue_R2.fastq threads: 24 resources: mem_mb=64000, mem_mib=61036, disk_mb=1000, disk_mib=954, tmpdir=, time=1440, javaAlloc=57600

    bbnorm.sh in=hecatomb.out/processing/assembly/unmapRescue_R1.fastq in2=hecatomb.out/processing/assembly/unmapRescue_R2.fastq             extra=hecatomb.out/processing/assembly/unmapRescue.s.fastq             out=hecatomb.out/processing/assembly/unmapRescueNorm_R1.fastq out2=hecatomb.out/processing/assembly/unmapRescueNorm_R2.fastq             target=100             ow=t             threads=24 -Xmx57600m 2> hecatomb.out/stderr/rescue_read_kmer_normalization.log
    rm hecatomb.out/stderr/rescue_read_kmer_normalization.log

sbatch: error: Batch job submission failed: Requested node configuration is not available Error submitting jobscript (exit code 1):

[Thu Mar 9 11:54:19 2023] rule cluster_similar_sequences: input: hecatomb.out/processing/temp/p04/A13-151-169-06_ATGTCA_R1.all.fastq output: hecatomb.out/processing/temp/p05/A13-151-169-06_ATGTCA_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-151-169-06_ATGTCA_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-151-169-06_ATGTCA_R1_all_seqs.fasta log: hecatomb.out/stderr/cluster_similar_sequences.A13-151-169-06_ATGTCA.log jobid: 3 benchmark: hecatomb.out/benchmarks/cluster_similar_sequences.A13-151-169-06_ATGTCA.txt reason: Missing output files: hecatomb.out/processing/temp/p05/A13-151-169-06_ATGTCA_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-151-169-06_ATGTCA_R1_cluster.tsv; Updated input files: hecatomb.out/processing/temp/p04/A13-151-169-06_ATGTCA_R1.all.fastq wildcards: sample=A13-151-169-06_ATGTCA threads: 24 resources: mem_mb=64000, mem_mib=61036, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    mmseqs easy-linclust hecatomb.out/processing/temp/p04/A13-151-169-06_ATGTCA_R1.all.fastq hecatomb.out/processing/temp/p05/A13-151-169-06_ATGTCA_R1 hecatomb.out/processing/temp/p05/A13-151-169-06_ATGTCA_TMP             --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3             --threads 24 &> hecatomb.out/stderr/cluster_similar_sequences.A13-151-169-06_ATGTCA.log
    rm hecatomb.out/stderr/cluster_similar_sequences.A13-151-169-06_ATGTCA.log

sbatch: error: Batch job submission failed: Requested node configuration is not available Error submitting jobscript (exit code 1):

[Thu Mar 9 11:54:19 2023] rule cluster_similar_sequences: input: hecatomb.out/processing/temp/p04/A13-255-183-06_GTGGCC_R1.all.fastq output: hecatomb.out/processing/temp/p05/A13-255-183-06_GTGGCC_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-255-183-06_GTGGCC_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-255-183-06_GTGGCC_R1_all_seqs.fasta log: hecatomb.out/stderr/cluster_similar_sequences.A13-255-183-06_GTGGCC.log jobid: 16 benchmark: hecatomb.out/benchmarks/cluster_similar_sequences.A13-255-183-06_GTGGCC.txt reason: Missing output files: hecatomb.out/processing/temp/p05/A13-255-183-06_GTGGCC_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-255-183-06_GTGGCC_R1_cluster.tsv; Updated input files: hecatomb.out/processing/temp/p04/A13-255-183-06_GTGGCC_R1.all.fastq wildcards: sample=A13-255-183-06_GTGGCC threads: 24 resources: mem_mb=64000, mem_mib=61036, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    mmseqs easy-linclust hecatomb.out/processing/temp/p04/A13-255-183-06_GTGGCC_R1.all.fastq hecatomb.out/processing/temp/p05/A13-255-183-06_GTGGCC_R1 hecatomb.out/processing/temp/p05/A13-255-183-06_GTGGCC_TMP             --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3             --threads 24 &> hecatomb.out/stderr/cluster_similar_sequences.A13-255-183-06_GTGGCC.log
    rm hecatomb.out/stderr/cluster_similar_sequences.A13-255-183-06_GTGGCC.log

sbatch: error: Batch job submission failed: Requested node configuration is not available Error submitting jobscript (exit code 1):

[Thu Mar 9 11:54:19 2023] rule cluster_similar_sequences: input: hecatomb.out/processing/temp/p04/A13-258-124-06_CGTACG_R1.all.fastq output: hecatomb.out/processing/temp/p05/A13-258-124-06_CGTACG_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-258-124-06_CGTACG_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-258-124-06_CGTACG_R1_all_seqs.fasta log: hecatomb.out/stderr/cluster_similar_sequences.A13-258-124-06_CGTACG.log jobid: 40 benchmark: hecatomb.out/benchmarks/cluster_similar_sequences.A13-258-124-06_CGTACG.txt reason: Missing output files: hecatomb.out/processing/temp/p05/A13-258-124-06_CGTACG_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-258-124-06_CGTACG_R1_rep_seq.fasta; Updated input files: hecatomb.out/processing/temp/p04/A13-258-124-06_CGTACG_R1.all.fastq wildcards: sample=A13-258-124-06_CGTACG threads: 24 resources: mem_mb=64000, mem_mib=61036, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    mmseqs easy-linclust hecatomb.out/processing/temp/p04/A13-258-124-06_CGTACG_R1.all.fastq hecatomb.out/processing/temp/p05/A13-258-124-06_CGTACG_R1 hecatomb.out/processing/temp/p05/A13-258-124-06_CGTACG_TMP             --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3             --threads 24 &> hecatomb.out/stderr/cluster_similar_sequences.A13-258-124-06_CGTACG.log
    rm hecatomb.out/stderr/cluster_similar_sequences.A13-258-124-06_CGTACG.log

sbatch: error: Batch job submission failed: Requested node configuration is not available Error submitting jobscript (exit code 1):

[Thu Mar 9 11:54:19 2023] rule cluster_similar_sequences: input: hecatomb.out/processing/temp/p04/A13-04-182-06_TAGCTT_R1.all.fastq output: hecatomb.out/processing/temp/p05/A13-04-182-06_TAGCTT_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-04-182-06_TAGCTT_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-04-182-06_TAGCTT_R1_all_seqs.fasta log: hecatomb.out/stderr/cluster_similar_sequences.A13-04-182-06_TAGCTT.log jobid: 22 benchmark: hecatomb.out/benchmarks/cluster_similar_sequences.A13-04-182-06_TAGCTT.txt reason: Missing output files: hecatomb.out/processing/temp/p05/A13-04-182-06_TAGCTT_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-04-182-06_TAGCTT_R1_cluster.tsv; Updated input files: hecatomb.out/processing/temp/p04/A13-04-182-06_TAGCTT_R1.all.fastq wildcards: sample=A13-04-182-06_TAGCTT threads: 24 resources: mem_mb=64000, mem_mib=61036, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    mmseqs easy-linclust hecatomb.out/processing/temp/p04/A13-04-182-06_TAGCTT_R1.all.fastq hecatomb.out/processing/temp/p05/A13-04-182-06_TAGCTT_R1 hecatomb.out/processing/temp/p05/A13-04-182-06_TAGCTT_TMP             --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3             --threads 24 &> hecatomb.out/stderr/cluster_similar_sequences.A13-04-182-06_TAGCTT.log
    rm hecatomb.out/stderr/cluster_similar_sequences.A13-04-182-06_TAGCTT.log

sbatch: error: Batch job submission failed: Requested node configuration is not available Error submitting jobscript (exit code 1):

[Thu Mar 9 11:54:19 2023] rule cluster_similar_sequences: input: hecatomb.out/processing/temp/p04/A13-256-117-06_ACTGAT_R1.all.fastq output: hecatomb.out/processing/temp/p05/A13-256-117-06_ACTGAT_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-256-117-06_ACTGAT_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-256-117-06_ACTGAT_R1_all_seqs.fasta log: hecatomb.out/stderr/cluster_similar_sequences.A13-256-117-06_ACTGAT.log jobid: 46 benchmark: hecatomb.out/benchmarks/cluster_similar_sequences.A13-256-117-06_ACTGAT.txt reason: Missing output files: hecatomb.out/processing/temp/p05/A13-256-117-06_ACTGAT_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-256-117-06_ACTGAT_R1_rep_seq.fasta; Updated input files: hecatomb.out/processing/temp/p04/A13-256-117-06_ACTGAT_R1.all.fastq wildcards: sample=A13-256-117-06_ACTGAT threads: 24 resources: mem_mb=64000, mem_mib=61036, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    mmseqs easy-linclust hecatomb.out/processing/temp/p04/A13-256-117-06_ACTGAT_R1.all.fastq hecatomb.out/processing/temp/p05/A13-256-117-06_ACTGAT_R1 hecatomb.out/processing/temp/p05/A13-256-117-06_ACTGAT_TMP             --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3             --threads 24 &> hecatomb.out/stderr/cluster_similar_sequences.A13-256-117-06_ACTGAT.log
    rm hecatomb.out/stderr/cluster_similar_sequences.A13-256-117-06_ACTGAT.log

sbatch: error: Batch job submission failed: Requested node configuration is not available Error submitting jobscript (exit code 1):

[Thu Mar 9 11:54:19 2023] rule cluster_similar_sequences: input: hecatomb.out/processing/temp/p04/A13-253-140-06_GTCCGC_R1.all.fastq output: hecatomb.out/processing/temp/p05/A13-253-140-06_GTCCGC_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-253-140-06_GTCCGC_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-253-140-06_GTCCGC_R1_all_seqs.fasta log: hecatomb.out/stderr/cluster_similar_sequences.A13-253-140-06_GTCCGC.log jobid: 28 benchmark: hecatomb.out/benchmarks/cluster_similar_sequences.A13-253-140-06_GTCCGC.txt reason: Missing output files: hecatomb.out/processing/temp/p05/A13-253-140-06_GTCCGC_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-253-140-06_GTCCGC_R1_cluster.tsv; Updated input files: hecatomb.out/processing/temp/p04/A13-253-140-06_GTCCGC_R1.all.fastq wildcards: sample=A13-253-140-06_GTCCGC threads: 24 resources: mem_mb=64000, mem_mib=61036, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    mmseqs easy-linclust hecatomb.out/processing/temp/p04/A13-253-140-06_GTCCGC_R1.all.fastq hecatomb.out/processing/temp/p05/A13-253-140-06_GTCCGC_R1 hecatomb.out/processing/temp/p05/A13-253-140-06_GTCCGC_TMP             --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3             --threads 24 &> hecatomb.out/stderr/cluster_similar_sequences.A13-253-140-06_GTCCGC.log
    rm hecatomb.out/stderr/cluster_similar_sequences.A13-253-140-06_GTCCGC.log

sbatch: error: Batch job submission failed: Requested node configuration is not available Error submitting jobscript (exit code 1):

[Thu Mar 9 11:54:19 2023] rule cluster_similar_sequences: input: hecatomb.out/processing/temp/p04/A13-256-115-06_GTTTCG_R1.all.fastq output: hecatomb.out/processing/temp/p05/A13-256-115-06_GTTTCG_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-256-115-06_GTTTCG_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-256-115-06_GTTTCG_R1_all_seqs.fasta log: hecatomb.out/stderr/cluster_similar_sequences.A13-256-115-06_GTTTCG.log jobid: 52 benchmark: hecatomb.out/benchmarks/cluster_similar_sequences.A13-256-115-06_GTTTCG.txt reason: Missing output files: hecatomb.out/processing/temp/p05/A13-256-115-06_GTTTCG_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-256-115-06_GTTTCG_R1_rep_seq.fasta; Updated input files: hecatomb.out/processing/temp/p04/A13-256-115-06_GTTTCG_R1.all.fastq wildcards: sample=A13-256-115-06_GTTTCG threads: 24 resources: mem_mb=64000, mem_mib=61036, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    mmseqs easy-linclust hecatomb.out/processing/temp/p04/A13-256-115-06_GTTTCG_R1.all.fastq hecatomb.out/processing/temp/p05/A13-256-115-06_GTTTCG_R1 hecatomb.out/processing/temp/p05/A13-256-115-06_GTTTCG_TMP             --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3             --threads 24 &> hecatomb.out/stderr/cluster_similar_sequences.A13-256-115-06_GTTTCG.log
    rm hecatomb.out/stderr/cluster_similar_sequences.A13-256-115-06_GTTTCG.log

sbatch: error: Batch job submission failed: Requested node configuration is not available Error submitting jobscript (exit code 1):

Trying to restart job 10. Trying to restart job 58. Trying to restart job 34. Trying to restart job 160. Trying to restart job 3. Trying to restart job 16. Trying to restart job 40. Trying to restart job 22. Trying to restart job 46. Trying to restart job 28. Trying to restart job 52. Select jobs to execute...

[Thu Mar 9 11:54:19 2023] rule cluster_similar_sequences: input: hecatomb.out/processing/temp/p04/A13-135-177-06_AGTTCC_R1.all.fastq output: hecatomb.out/processing/temp/p05/A13-135-177-06_AGTTCC_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-135-177-06_AGTTCC_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-135-177-06_AGTTCC_R1_all_seqs.fasta log: hecatomb.out/stderr/cluster_similar_sequences.A13-135-177-06_AGTTCC.log jobid: 10 benchmark: hecatomb.out/benchmarks/cluster_similar_sequences.A13-135-177-06_AGTTCC.txt reason: Missing output files: hecatomb.out/processing/temp/p05/A13-135-177-06_AGTTCC_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-135-177-06_AGTTCC_R1_rep_seq.fasta; Updated input files: hecatomb.out/processing/temp/p04/A13-135-177-06_AGTTCC_R1.all.fastq wildcards: sample=A13-135-177-06_AGTTCC threads: 24 resources: mem_mb=64000, mem_mib=61036, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    mmseqs easy-linclust hecatomb.out/processing/temp/p04/A13-135-177-06_AGTTCC_R1.all.fastq hecatomb.out/processing/temp/p05/A13-135-177-06_AGTTCC_R1 hecatomb.out/processing/temp/p05/A13-135-177-06_AGTTCC_TMP             --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3             --threads 24 &> hecatomb.out/stderr/cluster_similar_sequences.A13-135-177-06_AGTTCC.log
    rm hecatomb.out/stderr/cluster_similar_sequences.A13-135-177-06_AGTTCC.log

sbatch: error: Batch job submission failed: Requested node configuration is not available Error submitting jobscript (exit code 1):

[Thu Mar 9 11:54:19 2023] rule cluster_similar_sequences: input: hecatomb.out/processing/temp/p04/A13-12-250-06_GGCTAC_R1.all.fastq output: hecatomb.out/processing/temp/p05/A13-12-250-06_GGCTAC_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-12-250-06_GGCTAC_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-12-250-06_GGCTAC_R1_all_seqs.fasta log: hecatomb.out/stderr/cluster_similar_sequences.A13-12-250-06_GGCTAC.log jobid: 34 benchmark: hecatomb.out/benchmarks/cluster_similar_sequences.A13-12-250-06_GGCTAC.txt reason: Missing output files: hecatomb.out/processing/temp/p05/A13-12-250-06_GGCTAC_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-12-250-06_GGCTAC_R1_rep_seq.fasta; Updated input files: hecatomb.out/processing/temp/p04/A13-12-250-06_GGCTAC_R1.all.fastq wildcards: sample=A13-12-250-06_GGCTAC threads: 24 resources: mem_mb=64000, mem_mib=61036, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    mmseqs easy-linclust hecatomb.out/processing/temp/p04/A13-12-250-06_GGCTAC_R1.all.fastq hecatomb.out/processing/temp/p05/A13-12-250-06_GGCTAC_R1 hecatomb.out/processing/temp/p05/A13-12-250-06_GGCTAC_TMP             --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3             --threads 24 &> hecatomb.out/stderr/cluster_similar_sequences.A13-12-250-06_GGCTAC.log
    rm hecatomb.out/stderr/cluster_similar_sequences.A13-12-250-06_GGCTAC.log

sbatch: error: Batch job submission failed: Requested node configuration is not available Error submitting jobscript (exit code 1):

[Thu Mar 9 11:54:19 2023] rule cluster_similar_sequences: input: hecatomb.out/processing/temp/p04/A13-252-114-06_CCGTCC_R1.all.fastq output: hecatomb.out/processing/temp/p05/A13-252-114-06_CCGTCC_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-252-114-06_CCGTCC_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-252-114-06_CCGTCC_R1_all_seqs.fasta log: hecatomb.out/stderr/cluster_similar_sequences.A13-252-114-06_CCGTCC.log jobid: 58 benchmark: hecatomb.out/benchmarks/cluster_similar_sequences.A13-252-114-06_CCGTCC.txt reason: Missing output files: hecatomb.out/processing/temp/p05/A13-252-114-06_CCGTCC_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-252-114-06_CCGTCC_R1_cluster.tsv; Updated input files: hecatomb.out/processing/temp/p04/A13-252-114-06_CCGTCC_R1.all.fastq wildcards: sample=A13-252-114-06_CCGTCC threads: 24 resources: mem_mb=64000, mem_mib=61036, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    mmseqs easy-linclust hecatomb.out/processing/temp/p04/A13-252-114-06_CCGTCC_R1.all.fastq hecatomb.out/processing/temp/p05/A13-252-114-06_CCGTCC_R1 hecatomb.out/processing/temp/p05/A13-252-114-06_CCGTCC_TMP             --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3             --threads 24 &> hecatomb.out/stderr/cluster_similar_sequences.A13-252-114-06_CCGTCC.log
    rm hecatomb.out/stderr/cluster_similar_sequences.A13-252-114-06_CCGTCC.log

sbatch: error: Batch job submission failed: Requested node configuration is not available Error submitting jobscript (exit code 1):

[Thu Mar 9 11:54:19 2023] rule rescue_read_kmer_normalization: input: hecatomb.out/processing/assembly/unmapRescue_R1.fastq, hecatomb.out/processing/assembly/unmapRescue_R2.fastq, hecatomb.out/processing/assembly/unmapRescue.s.fastq output: hecatomb.out/processing/assembly/unmapRescueNorm_R1.fastq, hecatomb.out/processing/assembly/unmapRescueNorm_R2.fastq log: hecatomb.out/stderr/rescue_read_kmer_normalization.log jobid: 160 benchmark: hecatomb.out/benchmarks/rescue_read_kmer_normalization.txt reason: Missing output files: hecatomb.out/processing/assembly/unmapRescueNorm_R1.fastq, hecatomb.out/processing/assembly/unmapRescueNorm_R2.fastq; Updated input files: hecatomb.out/processing/assembly/unmapRescue_R1.fastq, hecatomb.out/processing/assembly/unmapRescue.s.fastq, hecatomb.out/processing/assembly/unmapRescue_R2.fastq threads: 24 resources: mem_mb=64000, mem_mib=61036, disk_mb=1000, disk_mib=954, tmpdir=, time=1440, javaAlloc=57600

    bbnorm.sh in=hecatomb.out/processing/assembly/unmapRescue_R1.fastq in2=hecatomb.out/processing/assembly/unmapRescue_R2.fastq             extra=hecatomb.out/processing/assembly/unmapRescue.s.fastq             out=hecatomb.out/processing/assembly/unmapRescueNorm_R1.fastq out2=hecatomb.out/processing/assembly/unmapRescueNorm_R2.fastq             target=100             ow=t             threads=24 -Xmx57600m 2> hecatomb.out/stderr/rescue_read_kmer_normalization.log
    rm hecatomb.out/stderr/rescue_read_kmer_normalization.log

sbatch: error: Batch job submission failed: Requested node configuration is not available Error submitting jobscript (exit code 1):

[Thu Mar 9 11:54:19 2023] rule cluster_similar_sequences: input: hecatomb.out/processing/temp/p04/A13-151-169-06_ATGTCA_R1.all.fastq output: hecatomb.out/processing/temp/p05/A13-151-169-06_ATGTCA_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-151-169-06_ATGTCA_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-151-169-06_ATGTCA_R1_all_seqs.fasta log: hecatomb.out/stderr/cluster_similar_sequences.A13-151-169-06_ATGTCA.log jobid: 3 benchmark: hecatomb.out/benchmarks/cluster_similar_sequences.A13-151-169-06_ATGTCA.txt reason: Missing output files: hecatomb.out/processing/temp/p05/A13-151-169-06_ATGTCA_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-151-169-06_ATGTCA_R1_cluster.tsv; Updated input files: hecatomb.out/processing/temp/p04/A13-151-169-06_ATGTCA_R1.all.fastq wildcards: sample=A13-151-169-06_ATGTCA threads: 24 resources: mem_mb=64000, mem_mib=61036, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    mmseqs easy-linclust hecatomb.out/processing/temp/p04/A13-151-169-06_ATGTCA_R1.all.fastq hecatomb.out/processing/temp/p05/A13-151-169-06_ATGTCA_R1 hecatomb.out/processing/temp/p05/A13-151-169-06_ATGTCA_TMP             --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3             --threads 24 &> hecatomb.out/stderr/cluster_similar_sequences.A13-151-169-06_ATGTCA.log
    rm hecatomb.out/stderr/cluster_similar_sequences.A13-151-169-06_ATGTCA.log

sbatch: error: Batch job submission failed: Requested node configuration is not available Error submitting jobscript (exit code 1):

[Thu Mar 9 11:54:19 2023] rule cluster_similar_sequences: input: hecatomb.out/processing/temp/p04/A13-255-183-06_GTGGCC_R1.all.fastq output: hecatomb.out/processing/temp/p05/A13-255-183-06_GTGGCC_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-255-183-06_GTGGCC_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-255-183-06_GTGGCC_R1_all_seqs.fasta log: hecatomb.out/stderr/cluster_similar_sequences.A13-255-183-06_GTGGCC.log jobid: 16 benchmark: hecatomb.out/benchmarks/cluster_similar_sequences.A13-255-183-06_GTGGCC.txt reason: Missing output files: hecatomb.out/processing/temp/p05/A13-255-183-06_GTGGCC_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-255-183-06_GTGGCC_R1_cluster.tsv; Updated input files: hecatomb.out/processing/temp/p04/A13-255-183-06_GTGGCC_R1.all.fastq wildcards: sample=A13-255-183-06_GTGGCC threads: 24 resources: mem_mb=64000, mem_mib=61036, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    mmseqs easy-linclust hecatomb.out/processing/temp/p04/A13-255-183-06_GTGGCC_R1.all.fastq hecatomb.out/processing/temp/p05/A13-255-183-06_GTGGCC_R1 hecatomb.out/processing/temp/p05/A13-255-183-06_GTGGCC_TMP             --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3             --threads 24 &> hecatomb.out/stderr/cluster_similar_sequences.A13-255-183-06_GTGGCC.log
    rm hecatomb.out/stderr/cluster_similar_sequences.A13-255-183-06_GTGGCC.log

sbatch: error: Batch job submission failed: Requested node configuration is not available Error submitting jobscript (exit code 1):

[Thu Mar 9 11:54:19 2023] rule cluster_similar_sequences: input: hecatomb.out/processing/temp/p04/A13-258-124-06_CGTACG_R1.all.fastq output: hecatomb.out/processing/temp/p05/A13-258-124-06_CGTACG_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-258-124-06_CGTACG_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-258-124-06_CGTACG_R1_all_seqs.fasta log: hecatomb.out/stderr/cluster_similar_sequences.A13-258-124-06_CGTACG.log jobid: 40 benchmark: hecatomb.out/benchmarks/cluster_similar_sequences.A13-258-124-06_CGTACG.txt reason: Missing output files: hecatomb.out/processing/temp/p05/A13-258-124-06_CGTACG_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-258-124-06_CGTACG_R1_rep_seq.fasta; Updated input files: hecatomb.out/processing/temp/p04/A13-258-124-06_CGTACG_R1.all.fastq wildcards: sample=A13-258-124-06_CGTACG threads: 24 resources: mem_mb=64000, mem_mib=61036, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    mmseqs easy-linclust hecatomb.out/processing/temp/p04/A13-258-124-06_CGTACG_R1.all.fastq hecatomb.out/processing/temp/p05/A13-258-124-06_CGTACG_R1 hecatomb.out/processing/temp/p05/A13-258-124-06_CGTACG_TMP             --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3             --threads 24 &> hecatomb.out/stderr/cluster_similar_sequences.A13-258-124-06_CGTACG.log
    rm hecatomb.out/stderr/cluster_similar_sequences.A13-258-124-06_CGTACG.log

sbatch: error: Batch job submission failed: Requested node configuration is not available Error submitting jobscript (exit code 1):

[Thu Mar 9 11:54:19 2023] rule cluster_similar_sequences: input: hecatomb.out/processing/temp/p04/A13-04-182-06_TAGCTT_R1.all.fastq output: hecatomb.out/processing/temp/p05/A13-04-182-06_TAGCTT_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-04-182-06_TAGCTT_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-04-182-06_TAGCTT_R1_all_seqs.fasta log: hecatomb.out/stderr/cluster_similar_sequences.A13-04-182-06_TAGCTT.log jobid: 22 benchmark: hecatomb.out/benchmarks/cluster_similar_sequences.A13-04-182-06_TAGCTT.txt reason: Missing output files: hecatomb.out/processing/temp/p05/A13-04-182-06_TAGCTT_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-04-182-06_TAGCTT_R1_cluster.tsv; Updated input files: hecatomb.out/processing/temp/p04/A13-04-182-06_TAGCTT_R1.all.fastq wildcards: sample=A13-04-182-06_TAGCTT threads: 24 resources: mem_mb=64000, mem_mib=61036, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    mmseqs easy-linclust hecatomb.out/processing/temp/p04/A13-04-182-06_TAGCTT_R1.all.fastq hecatomb.out/processing/temp/p05/A13-04-182-06_TAGCTT_R1 hecatomb.out/processing/temp/p05/A13-04-182-06_TAGCTT_TMP             --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3             --threads 24 &> hecatomb.out/stderr/cluster_similar_sequences.A13-04-182-06_TAGCTT.log
    rm hecatomb.out/stderr/cluster_similar_sequences.A13-04-182-06_TAGCTT.log

sbatch: error: Batch job submission failed: Requested node configuration is not available Error submitting jobscript (exit code 1):

[Thu Mar 9 11:54:19 2023] rule cluster_similar_sequences: input: hecatomb.out/processing/temp/p04/A13-256-117-06_ACTGAT_R1.all.fastq output: hecatomb.out/processing/temp/p05/A13-256-117-06_ACTGAT_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-256-117-06_ACTGAT_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-256-117-06_ACTGAT_R1_all_seqs.fasta log: hecatomb.out/stderr/cluster_similar_sequences.A13-256-117-06_ACTGAT.log jobid: 46 benchmark: hecatomb.out/benchmarks/cluster_similar_sequences.A13-256-117-06_ACTGAT.txt reason: Missing output files: hecatomb.out/processing/temp/p05/A13-256-117-06_ACTGAT_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-256-117-06_ACTGAT_R1_rep_seq.fasta; Updated input files: hecatomb.out/processing/temp/p04/A13-256-117-06_ACTGAT_R1.all.fastq wildcards: sample=A13-256-117-06_ACTGAT threads: 24 resources: mem_mb=64000, mem_mib=61036, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    mmseqs easy-linclust hecatomb.out/processing/temp/p04/A13-256-117-06_ACTGAT_R1.all.fastq hecatomb.out/processing/temp/p05/A13-256-117-06_ACTGAT_R1 hecatomb.out/processing/temp/p05/A13-256-117-06_ACTGAT_TMP             --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3             --threads 24 &> hecatomb.out/stderr/cluster_similar_sequences.A13-256-117-06_ACTGAT.log
    rm hecatomb.out/stderr/cluster_similar_sequences.A13-256-117-06_ACTGAT.log

sbatch: error: Batch job submission failed: Requested node configuration is not available Error submitting jobscript (exit code 1):

[Thu Mar 9 11:54:19 2023] rule cluster_similar_sequences: input: hecatomb.out/processing/temp/p04/A13-253-140-06_GTCCGC_R1.all.fastq output: hecatomb.out/processing/temp/p05/A13-253-140-06_GTCCGC_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-253-140-06_GTCCGC_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-253-140-06_GTCCGC_R1_all_seqs.fasta log: hecatomb.out/stderr/cluster_similar_sequences.A13-253-140-06_GTCCGC.log jobid: 28 benchmark: hecatomb.out/benchmarks/cluster_similar_sequences.A13-253-140-06_GTCCGC.txt reason: Missing output files: hecatomb.out/processing/temp/p05/A13-253-140-06_GTCCGC_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-253-140-06_GTCCGC_R1_cluster.tsv; Updated input files: hecatomb.out/processing/temp/p04/A13-253-140-06_GTCCGC_R1.all.fastq wildcards: sample=A13-253-140-06_GTCCGC threads: 24 resources: mem_mb=64000, mem_mib=61036, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    mmseqs easy-linclust hecatomb.out/processing/temp/p04/A13-253-140-06_GTCCGC_R1.all.fastq hecatomb.out/processing/temp/p05/A13-253-140-06_GTCCGC_R1 hecatomb.out/processing/temp/p05/A13-253-140-06_GTCCGC_TMP             --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3             --threads 24 &> hecatomb.out/stderr/cluster_similar_sequences.A13-253-140-06_GTCCGC.log
    rm hecatomb.out/stderr/cluster_similar_sequences.A13-253-140-06_GTCCGC.log

sbatch: error: Batch job submission failed: Requested node configuration is not available Error submitting jobscript (exit code 1):

[Thu Mar 9 11:54:19 2023] rule cluster_similar_sequences: input: hecatomb.out/processing/temp/p04/A13-256-115-06_GTTTCG_R1.all.fastq output: hecatomb.out/processing/temp/p05/A13-256-115-06_GTTTCG_R1_rep_seq.fasta, hecatomb.out/processing/temp/p05/A13-256-115-06_GTTTCG_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-256-115-06_GTTTCG_R1_all_seqs.fasta log: hecatomb.out/stderr/cluster_similar_sequences.A13-256-115-06_GTTTCG.log jobid: 52 benchmark: hecatomb.out/benchmarks/cluster_similar_sequences.A13-256-115-06_GTTTCG.txt reason: Missing output files: hecatomb.out/processing/temp/p05/A13-256-115-06_GTTTCG_R1_cluster.tsv, hecatomb.out/processing/temp/p05/A13-256-115-06_GTTTCG_R1_rep_seq.fasta; Updated input files: hecatomb.out/processing/temp/p04/A13-256-115-06_GTTTCG_R1.all.fastq wildcards: sample=A13-256-115-06_GTTTCG threads: 24 resources: mem_mb=64000, mem_mib=61036, disk_mb=1000, disk_mib=954, tmpdir=, time=1440

    mmseqs easy-linclust hecatomb.out/processing/temp/p04/A13-256-115-06_GTTTCG_R1.all.fastq hecatomb.out/processing/temp/p05/A13-256-115-06_GTTTCG_R1 hecatomb.out/processing/temp/p05/A13-256-115-06_GTTTCG_TMP             --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3             --threads 24 &> hecatomb.out/stderr/cluster_similar_sequences.A13-256-115-06_GTTTCG.log
    rm hecatomb.out/stderr/cluster_similar_sequences.A13-256-115-06_GTTTCG.log

sbatch: error: Batch job submission failed: Requested node configuration is not available Error submitting jobscript (exit code 1):

Exiting because a job execution failed. Look above for error message

FATAL: Hecatomb encountered an error.
       Dumping all error logs to "hecatomb.errorLogs.txt"Complete log: .snakemake/log/2023-03-09T115415.686966.snakemake.log

[2023:03:09 11:54:29] Error: Snakemake failed (base) [kvigil@cypress01-123 ~]$

katievigil commented 1 year ago

Hi I changed my config files to use 20 threads, but it looks like Hecatomb is still running on 24 threads. My HPC only allows 20 threads. What other files do I need to change? Here is my log:

I changed these config files under .config/snakemake/slurm hectomb.config.yaml config.yaml

And this file: conda-envs/hecatomb/snakemake/config

config.yaml

2023-03-09T131458.367041.snakemake.log

Thanks!

beardymcjohnface commented 1 year ago

If you're using a profile then --threads should be ignored. In your hecatomb.out/hecatomb.config.yaml can you change BigJobCpu: 24 to BigJobCpu: 20. You should also make sure BigJobMem is balanced for the cpu:memory ratio of the nodes. You can run sinfo -Nl to see that info for your cluster.

katievigil commented 1 year ago

Hi @beardymcjohnface I don't see the hecatomb.config.yaml in the hecatomb.out folder. This file is located in /home/kvigil/.config/snakemake/slurm

I changed the hecatomb.config.yaml file to reflect 20 threads:

BigJobCpu: 20 BigJobMem: 64000 BigJobTimeMin: 1440 COMPRESSION: 1 CONTIG_MINLENGTH: 1000 CUTTAIL_WINDOW: 25 DEDUP_ACCURACY: 4 Databases: null ENTROPY: 0.5 ENTROPYWINDOW: 25 Host: human MediumJobCpu: 16 MediumJobMem: 32000 MoreRamCpu: 2 MoreRamMem: 16000 Output: hecatomb.out Preprocess: paired QSCORE: 15 READ_MINLENGTH: 90 Reads: /lustre/project/taw/share/conda-envs/hecatomb/bin/../test_data Search: sensitive SmallJobCpu: 8 SmallJobMem: 16000 canuSettings: correctedErrorRate=0.16 maxInputCoverage=10000 minInputCoverage=0 corOutCoverage=10000 corMhapSensitivity=high corMinCoverage=0 useGrid=False stopOnLowCoverage=False genomeSize=10M -nanopore filtAAprimary: --min-length 30 -e 1e-3 filtAAsecondary: --min-length 30 -e 1e-5 filtNTprimary: --min-length 90 -e 1e-10 filtNTsecondary: --min-length 90 -e 1e-20 linclustParams: --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3 perfAA: --start-sens 1 --sens-steps 3 -s 7 --lca-mode 2 --shuffle 0 perfAAfast: -s 4.0 --lca-mode 2 --shuffle 0 perfNT: --start-sens 2 -s 7 --sens-steps 3 perfNTfast: -s 4.0 taxIdIgnore: 0 1 2 10239 131567 12429 2759

katievigil commented 1 year ago

2023-03-10T141950.676347.snakemake.log

Here is my log. It still says 24 threads even though I changed it.

beardymcjohnface commented 1 year ago

The /home/kvigil/.config/snakemake/slurm directory should contain config.yaml with the profile settings, which is different from the hecatomb.config.yaml, which should either be in your working directory or the hecatomb.out directory depending on the Hecatomb version. changing Hecatomb's default config (in /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../config/config.yaml) will only work once you've deleted your old hecatomb.config.yaml file. Do you have a hecatomb.config.yaml file in your working directory?

katievigil commented 1 year ago

Good news it worked when I changed the file to 20 threads! - It took about 6 hours to complete on HPC is that how long the test data usually take?

255 of 255 steps (100%) done Removing temporary output hecatomb.out/processing/mapping/assembly.seqtable.bam. Complete log: .snakemake/log/2023-03-14T113116.169580.snakemake.log

Hecatomb finished successfully!

[2023:03:14 17:59:12] Snakemake finished successfully

beardymcjohnface commented 1 year ago

That's great! yes unfortunately the current test dataset still takes quite a while. It's a small-ish dataset (10 samples with 25k paired reads), but it runs on the full size databases which takes a long time. We do need a much quicker test simulation, both for users and CI.

katievigil commented 1 year ago

@beardymcjohnface I am having a hard time now adding my host genome. hecatomb addHost --host oyster --hostfa H:\hecatomb \host_genomes\GCF_002022765.2_C_virginica-3.0_protein.faa.gz

██╗ ██╗███████╗ ██████╗ █████╗ ████████╗ ██████╗ ███╗ ███╗██████╗ ██║ ██║██╔════╝██╔════╝██╔══██╗╚══██╔══╝██╔═══██╗████╗ ████║██╔══██╗ ███████║█████╗ ██║ ███████║ ██║ ██║ ██║██╔████╔██║██████╔╝ ██╔══██║██╔══╝ ██║ ██╔══██║ ██║ ██║ ██║██║╚██╔╝██║██╔══██╗ ██║ ██║███████╗╚██████╗██║ ██║ ██║ ╚██████╔╝██║ ╚═╝ ██║██████╔╝ ╚═╝ ╚═╝╚══════╝ ╚═════╝╚═╝ ╚═╝ ╚═╝ ╚═════╝ ╚═╝ ╚═╝╚═════╝

Hecatomb version v1.1.0

Usage: hecatomb [OPTIONS] COMMAND [ARGS]... Try 'hecatomb --help' for help.

Error: No such command 'addHost'.

How do you recommend adding the host genome from NCBI?

katievigil commented 1 year ago

Hi,

Now I am getting an error when I run my sample:

(/lustre/project/taw/share/conda-envs/hecatomb) [kvigil@cypress2 ~]$ hecatomb run --preprocess longread --reads /lustre/project/taw/ONR030223/ONR030223/20230302_1344_MN18851_FAW54058_d4b97d63/fastq_pass/barcode07 --host oyster --threads 20

██╗ ██╗███████╗ ██████╗ █████╗ ████████╗ ██████╗ ███╗ ███╗██████╗ ██║ ██║██╔════╝██╔════╝██╔══██╗╚══██╔══╝██╔═══██╗████╗ ████║██╔══██╗ ███████║█████╗ ██║ ███████║ ██║ ██║ ██║██╔████╔██║██████╔╝ ██╔══██║██╔══╝ ██║ ██╔══██║ ██║ ██║ ██║██║╚██╔╝██║██╔══██╗ ██║ ██║███████╗╚██████╗██║ ██║ ██║ ╚██████╔╝██║ ╚═╝ ██║██████╔╝ ╚═╝ ╚═╝╚══════╝ ╚═════╝╚═╝ ╚═╝ ╚═╝ ╚═════╝ ╚═╝ ╚═╝╚═════╝

Hecatomb version v1.1.0

[2023:03:15 16:29:58] Config file hecatomb.config.yaml already exists. Using existing config file. [2023:03:15 16:29:58] Writing runtime config file to hecatomb.config.yaml [2023:03:15 16:29:58] ------------------ [2023:03:15 16:29:58] | Runtime config | [2023:03:15 16:29:58] ------------------

BigJobCpu: 24 BigJobMem: 64000 BigJobTimeMin: 1440 COMPRESSION: 1 CONTIG_MINLENGTH: 1000 CUTTAIL_WINDOW: 25 DEDUP_ACCURACY: 4 Databases: null ENTROPY: 0.5 ENTROPYWINDOW: 25 Host: oyster MediumJobCpu: 16 MediumJobMem: 32000 MoreRamCpu: 2 MoreRamMem: 16000 Output: hecatomb.out Preprocess: longread QSCORE: 15 READ_MINLENGTH: 90 Reads: /lustre/project/taw/ONR030223/ONR030223/20230302_1344_MN18851_FAW54058_d4b97d63/fastq_pass/barcode07 Search: sensitive SmallJobCpu: 8 SmallJobMem: 16000 canuSettings: correctedErrorRate=0.16 maxInputCoverage=10000 minInputCoverage=0 corOutCoverage=10000 corMhapSensitivity=high corMinCoverage=0 useGrid=False stopOnLowCoverage=False genomeSize=10M -nanopore filtAAprimary: --min-length 30 -e 1e-3 filtAAsecondary: --min-length 30 -e 1e-5 filtNTprimary: --min-length 90 -e 1e-10 filtNTsecondary: --min-length 90 -e 1e-20 linclustParams: --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3 perfAA: --start-sens 1 --sens-steps 3 -s 7 --lca-mode 2 --shuffle 0 perfAAfast: -s 4.0 --lca-mode 2 --shuffle 0 perfNT: --start-sens 2 -s 7 --sens-steps 3 perfNTfast: -s 4.0 taxIdIgnore: 0 1 2 10239 131567 12429 2759

[2023:03:15 16:29:58] --------------------- [2023:03:15 16:29:58] | Snakemake command | [2023:03:15 16:29:58] ---------------------

snakemake -s /lustre/project/taw/share/conda-envs/hecatomb/bin/../snakemake/workflow/Hecatomb.smk --configfile hecatomb.config.yaml --jobs 20 --use-conda --conda-frontend mamba --conda-prefix /lustre/project/taw/share/conda-envs/hecatomb/bin/../snakemake/conda --rerun-incomplete --printshellcmds --nolock --show-failed-logs Config file /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../config/config.yaml is extended by additional config specified via the command line. Config file /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../config/dbFiles.yaml is extended by additional config specified via the command line. Config file /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../config/immutable.yaml is extended by additional config specified via the command line. Building DAG of jobs... Your conda installation is not configured to use strict channel priorities. This is however crucial for having robust and correct environments (for details, see https://conda-forge.org/docs/user/tipsandtricks.html). Please consider to configure strict priorities by executing 'conda config --set channel_priority strict'. Using shell: /bin/bash Provided cores: 20 Rules claiming more threads will be scaled down. Job stats: job count min threads max threads

PRIMARY_AA_parsing 1 2 2 PRIMARY_AA_taxonomy_assignment 1 20 20 PRIMARY_NT_parsing 1 2 2 PRIMARY_NT_reformat 1 2 2 PRIMARY_NT_taxonomic_assignment 1 20 20 SECONDARY_AA_generate_output_table 1 2 2 SECONDARY_AA_parsing 1 2 2 SECONDARY_AA_refactor_finalize 1 2 2 SECONDARY_AA_taxonomy_assignment 1 20 20 SECONDARY_AA_tophit_lineage 1 2 2 SECONDARY_NT_convert 1 20 20 SECONDARY_NT_generate_output_table 1 2 2 SECONDARY_NT_summary 1 2 2 SECONDARY_NT_taxonomic_assignment 1 20 20 all 1 1 1 bam_index 1 2 2 calculate_gc 1 2 2 calculate_tet_freq 1 2 2 canu_sample_assembly 1 16 16 combine_AA_NT 1 1 1 combine_canu_contigs 1 1 1 concatentate_contig_count_tables 1 1 1 contig_krona_plot 1 1 1 contig_krona_text_format 1 1 1 contig_read_taxonomy 1 2 2 coverage_calculations 38 8 8 create_contig_count_table 38 1 1 krona_plot 1 1 1 krona_text_format 1 1 1 link_assembly 1 1 1 map_seq_table 1 16 16 mmseqs_contig_annotation 1 20 20 mmseqs_contig_annotation_summary 1 20 20 population_assembly 1 16 16 secondary_nt_calc_lca 1 20 20 secondary_nt_lca_table 1 1 1 seq_properties_table 2 2 2 tax_level_counts 1 2 2 total 113 1 20

Select jobs to execute...

[Wed Mar 15 16:30:09 2023] rule PRIMARY_AA_taxonomy_assignment: input: hecatomb.out/results/seqtable.fasta, /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/aa/virus_primary_aa/sequenceDB output: hecatomb.out/processing/mmseqs_aa_primary/MMSEQS_AA_PRIMARY_lca.tsv, hecatomb.out/processing/mmseqs_aa_primary/MMSEQS_AA_PRIMARY_report, hecatomb.out/processing/mmseqs_aa_primary/MMSEQS_AA_PRIMARY_tophit_report, hecatomb.out/processing/mmseqs_aa_primary/MMSEQS_AA_PRIMARY_tophit_aln, hecatomb.out/processing/mmseqs_aa_primary/MMSEQS_AA_PRIMARY_tophit_aln_sorted log: hecatomb.out/stderr/PRIMARY_AA_taxonomy_assignment.log jobid: 322 benchmark: hecatomb.out/benchmarks/PRIMARY_AA_taxonomy_assignment.txt reason: Updated input files: hecatomb.out/results/seqtable.fasta threads: 20 resources: tmpdir=/tmp, mem_mb=64000, mem_mib=61036, time=1440

    { # Run mmseqs taxonomy module
    mmseqs easy-taxonomy hecatomb.out/results/seqtable.fasta /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/aa/virus_primary_aa/sequenceDB hecatomb.out/processing/mmseqs_aa_primary/MMSEQS_AA_PRIMARY hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp             --min-length 30 -e 1e-3 --start-sens 1 --sens-steps 3 -s 7 --lca-mode 2 --shuffle 0 -a --tax-output-mode 2 --search-type 2 --tax-lineage 1 --lca-ranks "superkingdom,phylum,class,order,family,genus,species" --format-output "query,target,evalue,pident,fident,nident,mismatch,qcov,tcov,qstart,qend,qlen,tstart,tend,tlen,alnlen,bits,qheader,theader,taxid,taxname,taxlineage"             --threads 20 --split-memory-limit 48000M;

    # Add headers
    sort -k1 -n hecatomb.out/processing/mmseqs_aa_primary/MMSEQS_AA_PRIMARY_tophit_aln |             sed '1i query target   evalue  pident  fident  nident  mismatch        qcov    tcov    qstart  qend    qlen    tstart  tend    tlen   alnlen   bits    qheader theader taxid   taxname lineage' > hecatomb.out/processing/mmseqs_aa_primary/MMSEQS_AA_PRIMARY_tophit_aln_sorted;
    } &> hecatomb.out/stderr/PRIMARY_AA_taxonomy_assignment.log
    rm hecatomb.out/stderr/PRIMARY_AA_taxonomy_assignment.log

Activating conda environment: ../../lustre/project/taw/share/conda-envs/hecatomb/snakemake/conda/373dc37b9d1afb91f5ef6873a443b8ec_ [Wed Mar 15 16:53:00 2023] Error in rule PRIMARY_AA_taxonomy_assignment: jobid: 322 input: hecatomb.out/results/seqtable.fasta, /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/aa/virus_primary_aa/sequenceDB output: hecatomb.out/processing/mmseqs_aa_primary/MMSEQS_AA_PRIMARY_lca.tsv, hecatomb.out/processing/mmseqs_aa_primary/MMSEQS_AA_PRIMARY_report, hecatomb.out/processing/mmseqs_aa_primary/MMSEQS_AA_PRIMARY_tophit_report, hecatomb.out/processing/mmseqs_aa_primary/MMSEQS_AA_PRIMARY_tophit_aln, hecatomb.out/processing/mmseqs_aa_primary/MMSEQS_AA_PRIMARY_tophit_aln_sorted log: hecatomb.out/stderr/PRIMARY_AA_taxonomyassignment.log (check log file(s) for error details) conda-env: /lustre/project/taw/share/conda-envs/hecatomb/snakemake/conda/373dc37b9d1afb91f5ef6873a443b8ec shell:

    { # Run mmseqs taxonomy module
    mmseqs easy-taxonomy hecatomb.out/results/seqtable.fasta /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/aa/virus_primary_aa/sequenceDB hecatomb.out/processing/mmseqs_aa_primary/MMSEQS_AA_PRIMARY hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp             --min-length 30 -e 1e-3 --start-sens 1 --sens-steps 3 -s 7 --lca-mode 2 --shuffle 0 -a --tax-output-mode 2 --search-type 2 --tax-lineage 1 --lca-ranks "superkingdom,phylum,class,order,family,genus,species" --format-output "query,target,evalue,pident,fident,nident,mismatch,qcov,tcov,qstart,qend,qlen,tstart,tend,tlen,alnlen,bits,qheader,theader,taxid,taxname,taxlineage"             --threads 20 --split-memory-limit 48000M;

    # Add headers
    sort -k1 -n hecatomb.out/processing/mmseqs_aa_primary/MMSEQS_AA_PRIMARY_tophit_aln |             sed '1i query  target  evalue  pident  fident  nident  mismatch        qcov    tcov    qstart  qend    qlen     tstart  tend    tlen    alnlen  bits    qheader theader taxid   taxname lineage' > hecatomb.out/processing/mmseqs_aa_primary/MMSEQS_AA_PRIMARY_tophit_aln_sorted;
    } &> hecatomb.out/stderr/PRIMARY_AA_taxonomy_assignment.log
    rm hecatomb.out/stderr/PRIMARY_AA_taxonomy_assignment.log

    (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!)

Logfile hecatomb.out/stderr/PRIMARY_AA_taxonomy_assignment.log:

createdb hecatomb.out/results/seqtable.fasta hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/query --dbtype 0 --shuffle 0 --createdb-mode 1 --write-lookup 0 --id-offset 0 --compressed 0 -v 3

Converting sequences [=============== Time for merging to query_h: 0h 0m 0s 2ms Time for merging to query: 0h 0m 0s 1ms Database type: Nucleotide Time for processing: 0h 0m 0s 426ms Tmp hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp folder does not exist or is not a directory. extractorfs hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/query hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp/7817104469906507499/tmp_hsp1/7360049934769759246/q_orfs_aa --min-length 30 --max-length 32734 --max-gaps 2147483647 --contig-start-mode 2 --contig-end-mode 2 --orf-start-mode 1 --forward-frames 1,2,3 --reverse-frames 1,2,3 --translation-table 1 --translate 1 --use-all-table-starts 0 --id-offset 0 --create-lookup 0 --threads 20 --compressed 0 -v 3

[==================Invalid sequence with index 2003! ====Invalid sequence with index 55989! =Invalid sequence with index 63657! ===Invalid sequence with index 79056! ======Invalid sequence with index 64748! ==============Invalid sequence with index 13929! ==================Invalid sequence with index 128311! =] 151.76K 2s 965ms Time for merging to q_orfs_aa_h: 0h 0m 1s 105ms Time for merging to q_orfs_aa: 0h 0m 1s 838ms Time for processing: 0h 0m 8s 793ms prefilter hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp/7817104469906507499/tmp_hsp1/7360049934769759246/q_orfs_aa /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/aa/virus_primary_aa/sequenceDB hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp/7817104469906507499/tmp_hsp1/7360049934769759246/search/pref_0 --sub-mat nucl:nucleotide.out,aa:blosum62.out --seed-sub-mat nucl:nucleotide.out,aa:VTML80.out -k 0 --k-score 2147483647 --alph-size nucl:5,aa:21 --max-seq-len 65535 --max-seqs 300 --split 0 --split-mode 2 --split-memory-limit 48000M -c 0 --cov-mode 0 --comp-bias-corr 1 --diag-score 1 --exact-kmer-matching 0 --mask 1 --mask-lower-case 0 --min-ungapped-score 15 --add-self-matches 0 --spaced-kmer-mode 1 --db-load-mode 0 --pca 1 --pcb 1.5 --threads 20 --compressed 0 -v 3 -s 1.000

Query database size: 1773665 type: Aminoacid Estimated memory consumption: 6G Target database size: 1840337 type: Aminoacid Index table k-mer threshold: 154 at k-mer size 6 Index table: counting k-mers [=================================================================] 1.84M 37s 499ms Index table: Masked residues: 6082841 Index table: fill [=================================================================] 1.84M 12s 622ms Index statistics Entries: 281557312 DB size: 2099 MB Avg k-mer size: 4.399333 Top 10 k-mers TQFELG 248971 GWGPFQ 247784 DFENTQ 246686 NVPGGS 246673 RKTFPS 245401 YQNLGW 244983 SPIQMT 232436 GSLIHR 223178 IKKSWR 222897 HPGKKS 221025 Time for index table init: 0h 0m 52s 957ms Process prefiltering step 1 of 1

k-mer similarity threshold: 154 Starting prefiltering scores calculation (step 1 of 1) Query db start 1 to 1773665 Target db start 1 to 1840337 [=================================================================] 1.77M 16s 26ms

2.375376 k-mers per position 407 DB matches per sequence 0 overflows 0 queries produce too many hits (truncated result) 1 sequences passed prefiltering per query sequence 0 median result list length 1059700 sequences with 0 size result lists Time for merging to pref_0: 0h 0m 1s 477ms Time for processing: 0h 1m 15s 877ms align hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp/7817104469906507499/tmp_hsp1/7360049934769759246/q_orfs_aa /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/aa/virus_primary_aa/sequenceDB hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp/7817104469906507499/tmp_hsp1/7360049934769759246/search/pref_0 hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp/7817104469906507499/tmp_hsp1/7360049934769759246/search/aln_0 --sub-mat nucl:nucleotide.out,aa:blosum62.out -a 1 --alignment-mode 2 --wrapped-scoring 0 -e 0.001 --min-seq-id 0 --min-aln-len 0 --seq-id-mode 0 --alt-ali 0 -c 0 --cov-mode 0 --max-seq-len 65535 --comp-bias-corr 1 --realign 0 --max-rejected 2147483647 --max-accept 2147483647 --add-self-matches 0 --db-load-mode 0 --pca 1 --pcb 1.5 --score-bias 0 --gap-open nucl:5,aa:11 --gap-extend nucl:2,aa:1 --zdrop 40 --threads 20 --compressed 0 -v 3

Compute score, coverage and sequence identity Query database size: 1773665 type: Aminoacid Target database size: 1840337 type: Aminoacid Calculation of alignments [=================================================================] 1.77M 4s 24ms Time for merging to aln_0: 0h 0m 0s 734ms

2956412 alignments calculated. 151097 sequence pairs passed the thresholds (0.051108 of overall calculated). 0.085189 hits per query sequence. Time for processing: 0h 0m 20s 80ms createsubdb hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp/7817104469906507499/tmp_hsp1/7360049934769759246/search/order_0 hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp/7817104469906507499/tmp_hsp1/7360049934769759246/q_orfs_aa hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp/7817104469906507499/tmp_hsp1/7360049934769759246/search/input_0 -v 3 --subdb-mode 1

Time for merging to input_0: 0h 0m 0s 1ms Time for processing: 0h 0m 0s 479ms prefilter hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp/7817104469906507499/tmp_hsp1/7360049934769759246/search/input_0 /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/aa/virus_primary_aa/sequenceDB hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp/7817104469906507499/tmp_hsp1/7360049934769759246/search/pref_1 --sub-mat nucl:nucleotide.out,aa:blosum62.out --seed-sub-mat nucl:nucleotide.out,aa:VTML80.out -k 0 --k-score 2147483647 --alph-size nucl:5,aa:21 --max-seq-len 65535 --max-seqs 300 --split 0 --split-mode 2 --split-memory-limit 48000M -c 0 --cov-mode 0 --comp-bias-corr 1 --diag-score 1 --exact-kmer-matching 0 --mask 1 --mask-lower-case 0 --min-ungapped-score 15 --add-self-matches 0 --spaced-kmer-mode 1 --db-load-mode 0 --pca 1 --pcb 1.5 --threads 20 --compressed 0 -v 3 -s 4.0

Query database size: 1769959 type: Aminoacid Estimated memory consumption: 6G Target database size: 1840337 type: Aminoacid Index table k-mer threshold: 127 at k-mer size 6 Index table: counting k-mers [=================================================================] 1.84M 43s 425ms Index table: Masked residues: 6082841 Index table: fill [=================================================================] 1.84M 19s 793ms Index statistics Entries: 520624295 DB size: 3467 MB Avg k-mer size: 8.134755 Top 10 k-mers TQFELG 248971 GWGPFQ 247784 DFENTQ 246686 NVPGGS 246673 RKTFPS 245401 YQNLGW 244983 NNTGYQ 236377 VLVDFS 235418 NKTDEV 234993 VTLVAY 228872 Time for index table init: 0h 1m 13s 263ms Process prefiltering step 1 of 1

k-mer similarity threshold: 127 Starting prefiltering scores calculation (step 1 of 1) Query db start 1 to 1769959 Target db start 1 to 1840337 [=================================================================] 1.77M 58s 433ms

57.741859 k-mers per position 15904 DB matches per sequence 0 overflows 0 queries produce too many hits (truncated result) 65 sequences passed prefiltering per query sequence 41 median result list length 22847 sequences with 0 size result lists Time for merging to pref_1: 0h 0m 3s 484ms Time for processing: 0h 2m 34s 915ms align hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp/7817104469906507499/tmp_hsp1/7360049934769759246/search/input_0 /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/aa/virus_primary_aa/sequenceDB hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp/7817104469906507499/tmp_hsp1/7360049934769759246/search/pref_1 hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp/7817104469906507499/tmp_hsp1/7360049934769759246/search/aln_1 --sub-mat nucl:nucleotide.out,aa:blosum62.out -a 1 --alignment-mode 2 --wrapped-scoring 0 -e 0.001 --min-seq-id 0 --min-aln-len 0 --seq-id-mode 0 --alt-ali 0 -c 0 --cov-mode 0 --max-seq-len 65535 --comp-bias-corr 1 --realign 0 --max-rejected 2147483647 --max-accept 2147483647 --add-self-matches 0 --db-load-mode 0 --pca 1 --pcb 1.5 --score-bias 0 --gap-open nucl:5,aa:11 --gap-extend nucl:2,aa:1 --zdrop 40 --threads 20 --compressed 0 -v 3

Compute score, coverage and sequence identity Query database size: 1769959 type: Aminoacid Target database size: 1840337 type: Aminoacid Calculation of alignments [=================================================================] 1.77M 2m 0s 140ms Time for merging to aln_1: 0h 0m 5s 38ms

115062805 alignments calculated. 36106 sequence pairs passed the thresholds (0.000314 of overall calculated). 0.020399 hits per query sequence. Time for processing: 0h 2m 12s 467ms mergedbs hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp/7817104469906507499/tmp_hsp1/7360049934769759246/q_orfs_aa hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp/7817104469906507499/tmp_hsp1/7360049934769759246/search/aln_merge_new hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp/7817104469906507499/tmp_hsp1/7360049934769759246/search/aln_0 hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp/7817104469906507499/tmp_hsp1/7360049934769759246/search/aln_1 --compressed 0 -v 3

Merging the results to hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp/7817104469906507499/tmp_hsp1/7360049934769759246/search/aln_merge_new Time for merging to aln_merge_new: 0h 0m 0s 298ms Time for processing: 0h 0m 5s 423ms rmdb hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp/7817104469906507499/tmp_hsp1/7360049934769759246/search/aln_merge -v 3

Time for processing: 0h 0m 0s 15ms mvdb hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp/7817104469906507499/tmp_hsp1/7360049934769759246/search/aln_merge_new hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp/7817104469906507499/tmp_hsp1/7360049934769759246/search/aln_merge -v 3

Time for processing: 0h 0m 0s 8ms createsubdb hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp/7817104469906507499/tmp_hsp1/7360049934769759246/search/order_1 hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp/7817104469906507499/tmp_hsp1/7360049934769759246/search/input_0 hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp/7817104469906507499/tmp_hsp1/7360049934769759246/search/input_1 -v 3 --subdb-mode 1

Time for merging to input_1: 0h 0m 0s 1ms Time for processing: 0h 0m 0s 478ms prefilter hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp/7817104469906507499/tmp_hsp1/7360049934769759246/search/input_1 /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../../databases/aa/virus_primary_aa/sequenceDB hecatomb.out/processing/mmseqs_aa_primary/mmseqs_aa_tmp/8991890088055356121/taxonomy_tmp/7817104469906507499/tmp_hsp1/7360049934769759246/search/pref_2 --sub-mat nucl:nucleotide.out,aa:blosum62.out --seed-sub-mat nucl:nucleotide.out,aa:VTML80.out -k 0 --k-score 2147483647 --alph-size nucl:5,aa:21 --max-seq-len 65535 --max-seqs 300 --split 0 --split-mode 2 --split-memory-limit 48000M -c 0 --cov-mode 0 --comp-bias-corr 1 --diag-score 1 --exact-kmer-matching 0 --mask 1 --mask-lower-case 0 --min-ungapped-score 15 --add-self-matches 0 --spaced-kmer-mode 1 --db-load-mode 0 --pca 1 --pcb 1.5 --threads 20 --compressed 0 -v 3 -s 7.0

Query database size: 1768631 type: Aminoacid Estimated memory consumption: 6G Target database size: 1840337 type: Aminoacid Index table k-mer threshold: 100 at k-mer size 6 Index table: counting k-mers [=================================================================] 1.84M 44s 187ms Index table: Masked residues: 6082841 Index table: fill [=================================================================] 1.84M 50s 41ms Index statistics Entries: 522772222 DB size: 3479 MB Avg k-mer size: 8.168316 Top 10 k-mers TQFELG 248971 GWGPFQ 247784 DFENTQ 246686 NVPGGS 246673 RKTFPS 245401 YQNLGW 244983 NNTGYQ 236377 VLVDFS 235418 NKTDEV 234993 VTLVAY 228872 Time for index table init: 0h 1m 49s 749ms Process prefiltering step 1 of 1

k-mer similarity threshold: 100 Starting prefiltering scores calculation (step 1 of 1) Query db start 1 to 1768631 Target db start 1 to 1840337 [====================================================Can not write to data file e Error: Prefilter died Error: Search step died Error: First search died Error: Search died

Shutting down, this might take some time. Exiting because a job execution failed. Look above for error message

FATAL: Hecatomb encountered an error.
       Dumping all error logs to "hecatomb.errorLogs.txt"Complete log: .snakemake/log/2023-03-15T163002.627032.snakemake.log

[2023:03:15 16:53:00] Error: Snakemake failed

beardymcjohnface commented 1 year ago

@beardymcjohnface I am having a hard time now adding my host genome. hecatomb addHost --host oyster --hostfa H:\hecatomb \host_genomes\GCF_002022765.2_C_virginica-3.0_protein.faa.gz

██╗ ██╗███████╗ ██████╗ █████╗ ████████╗ ██████╗ ███╗ ███╗██████╗ ██║ ██║██╔════╝██╔════╝██╔══██╗╚══██╔══╝██╔═══██╗████╗ ████║██╔══██╗ ███████║█████╗ ██║ ███████║ ██║ ██║ ██║██╔████╔██║██████╔╝ ██╔══██║██╔══╝ ██║ ██╔══██║ ██║ ██║ ██║██║╚██╔╝██║██╔══██╗ ██║ ██║███████╗╚██████╗██║ ██║ ██║ ╚██████╔╝██║ ╚═╝ ██║██████╔╝ ╚═╝ ╚═╝╚══════╝ ╚═════╝╚═╝ ╚═╝ ╚═╝ ╚═════╝ ╚═╝ ╚═╝╚═════╝

Hecatomb version v1.1.0

Usage: hecatomb [OPTIONS] COMMAND [ARGS]... Try 'hecatomb --help' for help.

Error: No such command 'addHost'.

How do you recommend adding the host genome from NCBI?

This was my bad, the subcommand changed with an update. It's now add-host: hecatomb add-host ...

Make sure you download the nucleotide file, not the proteins.

beardymcjohnface commented 1 year ago

re: the mmseqs error, I'm not sure why it's died, there's only two similar issues that I could find on the mmseqs github. Do you have disk-space or inode quotas? and were you running this with a profile or as a local job? if you ran it with the example profile, there might be slurm logs in logs/PRIMARY_AA_taxonomy_assignment which should tell you if the scheduler killed it for some reason.

katievigil commented 1 year ago

@beardymcjohnface Here are all my logs,

For the 3/16/23 logs I used: hecatomb run --reads /lustre/project/taw/ONR030223/ONR030223/20230302_1344_MN18851_FAW54058_d4b97d63/fastq_pass/barcode07 --threads 20 --preprocess longread -- profile slurm --host oyster

2023-03-16T092821.856620.snakemake.log 2023-03-16T092747.012837.snakemake.log 2023-03-16T092055.486949.snakemake.log 2023-03-16T091947.073215.snakemake.log

Do I need to concatenate my fastq files for each barcode??

beardymcjohnface commented 1 year ago

Scheduler logs are handled separately, you should be able to find them in logs/PRIMARY_AA_taxonomy_assignment/. If it died due to memory or time limits it should state in these logs.

EDIT: Yes you should concatenate the fastq files for each barcode.

katievigil commented 1 year ago

@beardymcjohnface

I concatenated all the fastq.gz files and now I get this error:

(/lustre/project/taw/share/conda-envs/hecatomb) [kvigil@cypress01-119 barcode12]$ hecatomb run --reads /lustre/project/taw/ONR030223/ONR030223/20230302_1344_MN18851_FAW54058_d4b97d63/fastq_pass/concatenate/barcode01.fastq.gz --preprocess longread --threads 20 --profile slurm

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Hecatomb version v1.1.0

[2023:03:18 12:19:16] Config file hecatomb.config.yaml already exists. Using existing config file. [2023:03:18 12:19:16] Writing runtime config file to hecatomb.config.yaml [2023:03:18 12:19:16] ------------------ [2023:03:18 12:19:16] | Runtime config | [2023:03:18 12:19:16] ------------------

BigJobCpu: 20 BigJobMem: 64000 BigJobTimeMin: 1440 COMPRESSION: 1 CONTIG_MINLENGTH: 1000 CUTTAIL_WINDOW: 25 DEDUP_ACCURACY: 4 Databases: null ENTROPY: 0.5 ENTROPYWINDOW: 25 Host: human MediumJobCpu: 16 MediumJobMem: 32000 MoreRamCpu: 2 MoreRamMem: 16000 Output: hecatomb.out Preprocess: longread QSCORE: 15 READ_MINLENGTH: 90 Reads: /lustre/project/taw/ONR030223/ONR030223/20230302_1344_MN18851_FAW54058_d4b97d63/fastq_pass/concatenate/barcode01.fastq.gz Search: sensitive SmallJobCpu: 8 SmallJobMem: 16000 canuSettings: correctedErrorRate=0.16 maxInputCoverage=10000 minInputCoverage=0 corOutCoverage=10000 corMhapSensitivity=high corMinCoverage=0 useGrid=False stopOnLowCoverage=False genomeSize=10M -nanopore filtAAprimary: --min-length 30 -e 1e-3 filtAAsecondary: --min-length 30 -e 1e-5 filtNTprimary: --min-length 90 -e 1e-10 filtNTsecondary: --min-length 90 -e 1e-20 linclustParams: --kmer-per-seq-scale 0.3 -c 0.8 --cov-mode 1 --min-seq-id 0.97 --alignment-mode 3 perfAA: --start-sens 1 --sens-steps 3 -s 7 --lca-mode 2 --shuffle 0 perfAAfast: -s 4.0 --lca-mode 2 --shuffle 0 perfNT: --start-sens 2 -s 7 --sens-steps 3 perfNTfast: -s 4.0 taxIdIgnore: 0 1 2 10239 131567 12429 2759

[2023:03:18 12:19:16] --------------------- [2023:03:18 12:19:16] | Snakemake command | [2023:03:18 12:19:16] ---------------------

snakemake -s /lustre/project/taw/share/conda-envs/hecatomb/bin/../snakemake/workflow/Hecatomb.smk --configfile hecatomb.config.yaml --use-conda --conda-frontend mamba --conda-prefix /lustre/project/taw/share/conda-envs/hecatomb/bin/../snakemake/conda --rerun-incomplete --printshellcmds --nolock --show-failed-logs --profile slurm Config file /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../config/config.yaml is extended by additional config specified via the command line. Config file /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../config/dbFiles.yaml is extended by additional config specified via the command line. Config file /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/../config/immutable.yaml is extended by additional config specified via the command line. UnicodeDecodeError in file /lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/rules/00_samples_se.smk, line 34: 'utf-8' codec can't decode byte 0x8b in position 1: invalid start byte File "/lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/Hecatomb.smk", line 71, in File "/lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/rules/00_samples_se.smk", line 53, in parseSamples File "/lustre/project/taw/share/conda-envs/hecatomb/snakemake/workflow/rules/00_samples_se.smk", line 34, in samplesFromTsv File "/lustre/project/taw/share/conda-envs/hecatomb/lib/python3.10/codecs.py", line 322, in decode [2023:03:18 12:19:19] Error: Snakemake failed

beardymcjohnface commented 1 year ago

Sorry for the late reply, we had a hackathon last week. With --reads, you need to either pass a folder where the reads are located, or a TSV file that specifies the sample names and file paths. You also don't have to run them one barcode at a time. I would put the concatenated fastqs for each barcode into a new directory and pass that to --reads.

katievigil commented 1 year ago

Thanks! I will try this out!

katievigil commented 6 months ago

Hi, After a year I got this to work, I had to switch to the Loni supercomputer now its running! yey!

Load conda.sh script from the global conda installation

source /usr/local/packages/python/3.9.7-anaconda/etc/profile.d/conda.sh

create hecatomb conda environment

conda create -n hecatomb -c conda-forge -c bioconda hecatomb