sheenamt / msings

mSINGS: Detecting Microsatellite Instability By Next-Generation Sequencing This project provides a program with a command-line interface for detecting microsatellite instability by next-generation sequencing
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unable to create intervals file #1

Open elmaffo opened 4 years ago

elmaffo commented 4 years ago

I am trying to set up msings on a custom analysis. When I try to create the msi intervals file, target BED file sorting works but then the creation of the intervals file fails (the intervals file is empty) with the following messages:

Making MSI intervals file scripts/custom_regions.msi_intervals Traceback (most recent call last): File "/home/elmaffo/msings/msings-env/bin/msi", line 4, in import('pkg_resources').run_script('msings==191.302893', 'msi') File "/home/elmaffo/msings/msings-env/local/lib/python2.7/site-packages/pkg_resources/init.py", line 658, in run_script self.require(requires)[0].run_script(script_name, ns) File "/home/elmaffo/msings/msings-env/local/lib/python2.7/site-packages/pkg_resources/init.py", line 1438, in run_script exec(code, namespace, namespace) File "/home/elmaffo/msings/msings-env/lib/python2.7/site-packages/msings-191.302893-py2.7.egg/EGG-INFO/scripts/msi", line 7, in sys.exit(main(sys.argv[1:])) File "/home/elmaffo/msings/msings-env/local/lib/python2.7/site-packages/msings-191.302893-py2.7.egg/msings/scripts/script.py", line 32, in main return action(arguments) File "/home/elmaffo/msings/msings-env/local/lib/python2.7/site-packages/msings-191.302893-py2.7.egg/msings/subcommands/formatter.py", line 57, in action chr, beg, end = coords(row) File "/home/elmaffo/msings/msings-env/local/lib/python2.7/site-packages/msings-191.302893-py2.7.egg/msings/subcommands/formatter.py", line 32, in coords return row[chromosome], int(row[start]), int(row[stop]) ValueError: invalid literal for int() with base 10: 'Start' Created scripts/custom_regions.msi_intervals

elmaffo commented 4 years ago

Addendum: the bed file is in format chr# start stop, not # start stop