"The junction-spanning reads (JSRs) were categorized by the position of 50and 30site positions. A JSR was marked as a leader-to-body junction when the 5' site of the deletion is mapped to a genomic position between 55 and 85. In the cases where the 50site is inthe 5' UTR region, the sgRNA identity and the frame matching were determined by the first appearance of AUG in the downstream ofthe 3' site. In the cases where the 5' site is in a known ORF or an AUG is introduced by fusion, we checked if the concatenatedsequence generates a protein product with the same reading frame as a canonical ORF after the 3' site"
"For the analyses of sgRNA reads using the nanopore DRS data, the mapped reads from canonical sgRNAs were identified using the start and end positions of large deletions >= 10000 nt. For a valid assignment to a species of sgRNA, we required that the start position is between 55 and 85 in the genomic coordinate. The first AUG in the downstream of the end position of a large deletion was used for identification of the ‘‘spliced’’ product"
"The junction-spanning reads (JSRs) were categorized by the position of 50and 30site positions. A JSR was marked as a leader-to-body junction when the 5' site of the deletion is mapped to a genomic position between 55 and 85. In the cases where the 50site is inthe 5' UTR region, the sgRNA identity and the frame matching were determined by the first appearance of AUG in the downstream ofthe 3' site. In the cases where the 5' site is in a known ORF or an AUG is introduced by fusion, we checked if the concatenatedsequence generates a protein product with the same reading frame as a canonical ORF after the 3' site"
"For the analyses of sgRNA reads using the nanopore DRS data, the mapped reads from canonical sgRNAs were identified using the start and end positions of large deletions >= 10000 nt. For a valid assignment to a species of sgRNA, we required that the start position is between 55 and 85 in the genomic coordinate. The first AUG in the downstream of the end position of a large deletion was used for identification of the ‘‘spliced’’ product"