sheynkman-lab / Long-Read-Proteogenomics

A workflow for enhanced protein isoform detection through integration of long-read RNA-seq and mass spectrometry-based proteomics.
MIT License
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metamorpheus and rescue+resolve #168

Open rsalz opened 1 year ago

rsalz commented 1 year ago

I would like to use metamorpheus + rescue and resolve, so I'm having a look at main_metamorpheus.nf to try to run it.

Some questions: 1 - can't find the toml file that you use. Why do you only run 1 TOML when metamopheus recommends 5 TOML files? 2- why are you converting .raw with msconvert? Metamorpheus can take raw files as input. 3- I have an experimental design tsv that works with metamorpheus for normalization purposes, where does this fit in 4- generally how to run this nextflow file by itself, isn't clear by looking at the notes in the file

Thanks!