Open ShaowenJ opened 8 months ago
Hi ShaowenJ, Thanks for your question.
At the moment BLAE is only compatible with 10x workflow. If you provide some more detail re the structure of your read we may be able to help modify the workflow.
All the best
Sefi
Hi, I have a similar request. Is it possible to set a certain barcode length? Or that the BC length is taken from the supplied whitelist?
Cheers, Luuk
Hi @ljwharbers, as BLAZE was originally designed mainly for the 10X kits, the barcode length was hard coded. I can certainly make it specifiable in the future version. For now, if you do want to modify the default barcode length, the only way I can think of is as follows:
main
branch)blaze/config.py
: DEFAULT_BC_SIZE = 16
setup.py
) and install the modified version by pip install .
Dear BLAZE developers,
I was wondering if the BLAZE pipeline can be used with a different single-cell protocol than 10X. If our single-cell protocol has a different Adapter sequence, and we have 2 barcode sequences on both sides of the Adapter, would the pipeline also works?
Thanks, Look forward to your reply!