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shimlab / BLAZE

SingleCell Nanopore sequencing data analysis
GNU General Public License v3.0
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Split-seq single cell barcoding method demultipexing #21

Open vasikara17 opened 1 month ago

vasikara17 commented 1 month ago

Hello I would like to ask if BLAZE can work with other type of single cell data such as split-seq technology. These barcodes are a bit different than the 10X ones, since this technology uses 3 different barcodes and is based on pooling splitting ligation steps.

Best regards, Vasiliki

youyupei commented 2 weeks ago

Hi @vasikara17, unfortunately, does not work with Split-seq at the moment but only compatible with 10X data. Alternatively, I think there are other software which can do this (e.g. flexiplex https://davidsongroup.github.io/flexiplex/)