I ran it on a ~150 GB BAM file and it finished in about 30 seconds or so which I think is far too quick and did not provide any entries in bp.fa. The mapped.sam and the all.bed just contain header information, despite reporting success. Any idea what is going on?
Lowering the min BP cluster size didn't add any more results, still nothing.
How fast does this program run?
I ran it on a ~150 GB BAM file and it finished in about 30 seconds or so which I think is far too quick and did not provide any entries in bp.fa. The mapped.sam and the all.bed just contain header information, despite reporting success. Any idea what is going on?
Lowering the min BP cluster size didn't add any more results, still nothing.
-John
INPUT INFORMATION
SAM FILE : X306_Merged_Lanes_realigned.bam
FASTA FILE : ./hg19.fa
JSON FILE : null
OUTPUT DIR : X306
BP FILTER PROCESSES : 2
MAX BREAKPOINT DIFF : 2
MIN BP CLUSTER SIZE : 5
MIN MEAN BASE QUAL : 5
MIN SEQ LENGTH : 10
BASES AROUND BREAK : 1000
MAX SV DIFF : 10
MIN SV CLUSTER SIZE : 10
MAPPER : bwa
MAPPER THREADS : 2
node /lustre/scratch/ozborn/schroeder/ngs/clipcrop/shinout-clipcrop-421d175/subs/rawbreaks.js X306_Merged_Lanes_realigned.bam --parallel 2 node /lustre/scratch/ozborn/schroeder/ngs/clipcrop/shinout-clipcrop-421d175/subs/bpfastagen.js X306/bp.bed ./hg19.fa -l 1000 -j bwa index X306/bp.fa bwa aln -t 2 X306/bp.fa X306/bp.fastq >X306/mapped.sai bwa samse -f X306/mapped.sam X306/bp.fa X306/mapped.sai X306/bp.fastq node /lustre/scratch/ozborn/schroeder/ngs/clipcrop/shinout-clipcrop-421d175/subs/cluster_svinfo.js ./hg19.fa X306 10 10 SUCCEEDED