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shiquan / PISA

A collection of tools to process single-cell omics datasets.
MIT License
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PISA count error #14

Open liufuyan2016 opened 1 month ago

liufuyan2016 commented 1 month ago

I use the following command for big genome (wheat) scRNA-seq analysis: PISA count -one-hit -@ 10 -cb CB -anno-tag GN -umi UB -outdir RawMatrix final.sorted.bam An error occurred: [error] [func: write_outs, line: 431] No barcode found.

Could you help me? Thank you!

shiquan commented 1 month ago

Could you check if the CB tag exists in the bam file? samtools view final.sorted.bam | grep 'CB:Z:'