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shizhuoxing / BGI-Full-Length-RNA-Analysis-Pipeline

Full-Length RNA Analysis pipeline developted by BGI RD group.
https://github.com/shizhuoxing/BGI-Full-Length-RNA-Analysis-Pipeline
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Why the number of sequences in ccs.fasta is less than the number in flnc.fasta? #5

Closed Moo-cow closed 1 year ago

Moo-cow commented 1 year ago

Hi , I took the iso-seq at BGI. I used the official PacBio pipeline flnc.fasta file which has only a hundred sequences, which is obviously not correct. I used your pipeline and found that the number of sequences in ccs.fasta is less than the number in flnc.fasta. Please tell me is this normal? Thanks.

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shizhuoxing commented 1 year ago

Hi, That's because we're using BGI patented IsoLink-seq protocol: https://github.com/shizhuoxing/BGI-Full-Length-RNA-Analysis-Pipeline/wiki.

Moo-cow commented 1 year ago

Wow~ Thank you !