Error during annotation

Hi there! I am using TACCO to annotate my spatial single cell data with a pubblished datasets. In particular, I am having issues with this dataset. I would like to ask and to understand which is the problem and I can solve it.
Best regards, Giulia L.
` import tacco as tc
tc.tl.annotate(adata, reference, annotation_key='tissue_type',counts_location='X', result_key='cell_type',max_annotation=1,assume_valid_counts=True,multi_center=0)`

Annotation profiles were not found in `reference.varm["tissue_type"]`. Constructing reference profiles with `tacco.preprocessing.construct_reference_profiles` and default arguments...
Finished preprocessing in 6.31 seconds.
There is no expression data available in the `reference` (`np.prod(reference.shape) == 0`)! Therefore no platform normalization is performed even though `platform_iterations=0`.
Starting annotation of data with shape (126176, 0) and a reference of shape (79522, 0) using the following wrapped method:
+- maximum annotation: max_annotation=1
   +- multi center: multi_center=0 multi_center_amplitudes=True
      +- bisection boost: bisections=4, bisection_divisor=3
         +- core: method=OT annotation_prior=None
bisection run on 1
---------------------------------------------------------------------------
ValueError                                Traceback (most recent call last)
Cell In[18], line 3
      1 import tacco as tc
      2 #tc.tl.annotate(adata, reference, annotation_key='Detailed_Cell_Type', result_key='cell_type',max_annotation=1,assume_valid_counts=True,method='svm')
----> 3 tc.tl.annotate(adata, reference, annotation_key='tissue_type',counts_location='X', result_key='cell_type',max_annotation=1,assume_valid_counts=True,multi_center=0)

File [~/.conda/envs/seurat/lib/python3.9/site-packages/tacco/tools/_annotate.py:820](http://localhost:50188/~/.conda/envs/seurat/lib/python3.9/site-packages/tacco/tools/_annotate.py#line=819), in annotate(adata, reference, annotation_key, result_key, counts_location, method, bisections, bisection_divisor, platform_iterations, normalize_to, annotation_prior, multi_center, multi_center_amplitudes, reconstruction_key, max_annotation, min_counts_per_gene, min_counts_per_cell, min_cells_per_gene, min_genes_per_cell, remove_constant_genes, remove_zero_cells, min_log2foldchange, min_expression, remove_mito, n_hvg, skip_checks, assume_valid_counts, return_reference, gene_keys, verbose, **kw_args)
    818     print('\n'.join(method_construction_info[::-1]))
    819 start = time.time()
--> 820 cell_type = _method(tdata, reference, annotation_key, annotation_prior, verbose)
    821 if verbose > 0:
    822     print(f'Finished annotation in {np.round(time.time() - start, 2)} seconds.')

File [~/.conda/envs/seurat/lib/python3.9/site-packages/tacco/tools/_annotate.py:499](http://localhost:50188/~/.conda/envs/seurat/lib/python3.9/site-packages/tacco/tools/_annotate.py#line=498), in max_annotation_method.<locals>._method(adata, reference, annotation_key, annotation_prior, verbose)
    495 def _method(adata, reference, annotation_key, annotation_prior, verbose):
    497     nonlocal annotation_method, max_annotation, prepare_reconstruction
--> 499     cell_type = annotation_method(adata, reference, annotation_key, annotation_prior, verbose)
    501     if max_annotation is not None and max_annotation > 0:
    502         _cell_type = cell_type.to_numpy()

File [~/.conda/envs/seurat/lib/python3.9/site-packages/tacco/tools/_annotate.py:381](http://localhost:50188/~/.conda/envs/seurat/lib/python3.9/site-packages/tacco/tools/_annotate.py#line=380), in multi_center_annotation_method.<locals>._method(adata, reference, annotation_key, annotation_prior, verbose)
    378 nonlocal annotation_method, multi_center, prepare_reconstruction
    380 if multi_center is None or multi_center <= 1:
--> 381     cell_type = annotation_method(adata, reference, annotation_key, annotation_prior, verbose)
    382     if prepare_reconstruction is not None:
    383         prepare_reconstruction['annotation'] = cell_type.copy()

File [~/.conda/envs/seurat/lib/python3.9/site-packages/tacco/tools/_annotate.py:208](http://localhost:50188/~/.conda/envs/seurat/lib/python3.9/site-packages/tacco/tools/_annotate.py#line=207), in boost_annotation_method.<locals>._method(adata, reference, annotation_key, annotation_prior, verbose)
    205 if verbose > 0:
    206     print(f'bisection run on {todo}')
--> 208 cell_type = annotation_method(adata, reference, annotation_key, annotation_prior, verbose)
    210 #print('update result with weight', current)
    211 cell_type *= 1 / cell_type.sum(axis=1).to_numpy()[:, None]

File [~/.conda/envs/seurat/lib/python3.9/site-packages/tacco/tools/_annotate.py:115](http://localhost:50188/~/.conda/envs/seurat/lib/python3.9/site-packages/tacco/tools/_annotate.py#line=114), in annotation_method.<locals>._method(adata, reference, annotation_key, annotation_prior, verbose)
    112     else:
    113         _annotation_prior = annotation_prior(adata, reference, annotation_key)
--> 115     cell_type = annotate(adata, reference, annotation_key, annotation_prior=_annotation_prior, **verbose_arg, **kw_args)
    117     #cell_type/cell_type.sum(axis=1)[:,None]
    118 else:
    119     cell_type = annotate(adata, reference, annotation_key, **verbose_arg, **kw_args)

File [~/.conda/envs/seurat/lib/python3.9/site-packages/tacco/tools/_OT.py:66](http://localhost:50188/~/.conda/envs/seurat/lib/python3.9/site-packages/tacco/tools/_OT.py#line=65), in _annotate_OT(adata, reference, annotation_key, annotation_prior, epsilon, lamb, decomposition, deconvolution, **kw_args)
     59 """\
     60 Implements the functionality of :func:`~annotate_OT` without data
     61 integrity checks.
     62 """
     64 cell_prior = helper.prep_cell_priors(adata, reads=True)
---> 66 type_cell_dist = helper.prep_distance(adata, reference, annotation_key, decomposition=decomposition, deconvolution=deconvolution, **kw_args)
     67 if decomposition:
     68     type_cell_dist, mixtures = type_cell_dist

File [~/.conda/envs/seurat/lib/python3.9/site-packages/tacco/tools/_helper.py:74](http://localhost:50188/~/.conda/envs/seurat/lib/python3.9/site-packages/tacco/tools/_helper.py#line=73), in prep_distance(tdata, reference, type_key, n_pca, zero_center, log_norm, scale, metric, min_distance, decomposition, deconvolution)
     71     raise ValueError('The deconvolution and decomposition workflow dont work together!')
     73 if (np.array(tdata.X.sum(axis=1)).flatten()==0).any():
---> 74     raise ValueError('There are observations without non-zero variables!')
     76 tdata, reference = tdata.copy(), reference.copy() # dont touch the originals
     78 if log_norm:

ValueError: There are observations without non-zero variables!
simonwm / tacco

Error during annotation #18
