Open mujiezhang opened 2 years ago
Hi,
This looks like a format issue in the GenBank file you are trying to use as input, as there is a missing tag ("locus_tag" should be in there). My recommendation would be to try to run the pipeline from a fasta file of the same sequence instead ?
If you are already doing this, then I am not sure what happens, and would need to see the output folder.
Best, Simon
Thanks for your suggestion!
Yes, you can use Identify_candidate_fragments_from_fna.pl instead of Identify_candidate_fragments_from_gbk.pl in the first step (see https://github.com/simroux/Inovirus/tree/master/Inovirus_detector#example-with-a-fasta-file-as-input)
I am not sure I understand the question. This set of script is an automated inovirus detector, but it is expected that the exact boundaries will not always be found. The tool in this case indicates that it could not identify att sites, so any boundary should be interpreted as "possible" at best. If there are better / refined coordinates for this inovirus, these should be used. And as you mention, any analysis (and specifically results interpretation) should be careful and always take into consideration that prophage boundaries were identified by an automatic tool and thus likely to include some errors.
I got it. Thank you very much for your kindly help !
When I run the script "Identify_candidate_fragments_from_gbk.pl" , I got an exception like this: ------------- EXCEPTION ------------- MSG: asking for tag value that does not exist locus_tag STACK Bio::SeqFeature::Generic::get_tag_values /dssg/home/acct-clsjhh/clsjhh/anaconda3/envs/inovirus_detector/lib/perl5/site_perl/Bio/SeqFeature/Generic.pm:604 STACK toplevel /dssg/home/acct-clsjhh/clsjhh/zmj/software/inovirus_detector/srouxjgi-inovirus-dfc3d5c3b1ac/Inovirus_detector/Identify_candidate_fragments_from_gbk.pl:120 So what is the problem? Thanks a lot