single-cell-genetics / vireo

Demultiplexing pooled scRNA-seq data with or without genotype reference
https://vireoSNP.readthedocs.io
Apache License 2.0
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Error when using --forceLearGT #5

Open drneavin opened 5 years ago

drneavin commented 5 years ago

Hello,

I have been running vireoSNP v0.1.8 with a variety of different commands to compare the results. I have genotype information for some but not all of my multiplexed samples. When I run: vireo -c $CELL_DATA -d $DONOR_GT_FILE -o $OUT -N $n_donor --forceLearnGT

I get the following error:

Warning: no outDir provided, we use $cellFilePath/vireo.
Traceback (most recent call last):
  File "/share/ClusterShare/software/contrib/sccg/anaconda3/bin/vireo", line 11, in <module>
    load_entry_point('vireoSNP==0.1.8', 'console_scripts', 'vireo')()
  File "/share/ClusterShare/software/contrib/sccg/anaconda3/lib/python3.7/site-packages/vireoSNP-0.1.8-py3.7.egg/vireoSNP/vireo.py", line 87, in main
AttributeError: 'Values' object has no attribute 'cell_file'

Even though I have provided an outDir in the command it seems to think that I have not.

I have run: vireo -c $CELL_DATA -d $DONOR_GT_FILE -o $OUT -N $n_donor without this error (but with a different error that was posted separately)

and I also ran: vireo -c $CELL_DATA -o $OUT -N $n_donor that finished without any errors.

Any ideas on how I might fix this?

Cheers, Drew