singleron-RD / CeleScope

Single Cell Analysis Pipelines
https://www.singleron.bio/
MIT License
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OpenBLAS warning: precompiled NUM_THREADS exceeded, adding auxiliary array for thread metadata. #293

Open haikunhu opened 2 months ago

haikunhu commented 2 months ago

When I run the .sh script generated by multi_RNA, the following error occurs: OpenBLAS warning: precompiled NUM_THREADS exceeded, adding auxiliary array for thread metadata. To avoid this warning, please rebuild your copy of OpenBLAS with a larger NUM_THREADS setting or set the environment variable OPENBLAS_NUM_THREADS to 64 or lower Segmentation fault (core dumped) Data from a certain sample in the same batch of data can be mapped successfully, while some sample encountered the error above. How can I fix this? Version celescope 2.0.7

zhouyiqi91 commented 2 months ago

https://stackoverflow.com/questions/75619847/python-sklearn-openblas-error-for-kmeans

Try to add export OPENBLAS_NUM_THREADS=1 at the top of the shell script.

haikunhu commented 2 months ago

https://stackoverflow.com/questions/75619847/python-sklearn-openblas-error-for-kmeans

Try to add export OPENBLAS_NUM_THREADS=1 at the top of the shell script.

Thank you so much for this solution and it worked. I ran into another error when mapping other samples from the same batch of data. 微信图片_20240923170809 All the samples were prepared with the same kit (Singleron Biotechnologies, 4180011) and the .sh script were generated by the same multi_RNA command. I'd appreciate it if you could fix this again.

zhouyiqi91 commented 2 months ago

Exit status 102 usually means an error related to memory or disk. https://github.com/alexdobin/STAR/issues/1236

I think the real error message of the STAR should be a little above this screenshot.

zhouyiqi91 commented 2 months ago

Add the paramter --STAR_param "--limitBAMsortRAM 20000000000" to multi_rna.

haikunhu commented 2 months ago

Add the paramter --STAR_param "--limitBAMsortRAM 20000000000" to multi_rna.

Problems solved! Thank you so much and you can close this issue.