Sequencing fails

[GoogleCodeExporter]

Dear Micheal,
I've tried Flux Simulator, but found that it didn't generate any reads. I used 
Flux Simulator as the instruction at 
http://flux.sammeth.net/sim_gene_expression.html,  but modified .pro generated 
by the "expression" step manually before I conducted the "Library" step. After 
I conducted the "Sequencing" step, the indicator at the "Sequencing" tag 
remained red and the .bed and .fasta files are both empty, but the .lib file 
was OK.  

The attachments are the .par .gtf and .pro files I used.  Is that a bug or I 
failed to use it correctly? Thanks a lot.

The enviroment I conducted the experiment is :

Flux Simulator version : 20100427
OS : Window vista
processor : 2.2 GHz
RAM : 4 Gb

Best.

Zhixing 

Original issue reported on code.google.com by gmicha@gmail.com on 22 Jun 2010 at 11:49

• Merged into: #43

[GoogleCodeExporter]

Dear Zhixing,

I failed to reproduce the problem with the current build 20100611 of the 
FluxSimulator on a Windows 64bit machine here: your par/pro/gtf file produced 
an ~30Mb .lib file, which subsequently produced .bed and .fasta files with the 
desired amount of reads. 

This does not mean that the problem does not exist. Please confirm if your 
problem persists using the newest build. If so, provide some more information 
on what the program does after the launching the sequencing step (i.e., do you 
see progress bars at the bottom in the status bar, what do the labels read, 
etc. ..). You may also try to launch the wrapper script from a command shell 
(Start->Execute->cmd)

flux.bat -sp dummy.par

Issue stays open until further clarification.

Original comment by gmicha@gmail.com on 22 Jun 2010 at 11:57

• Changed state: Unconfirmed

[GoogleCodeExporter]

Due to similarity of described symptoms, I merged with issue 43.

Original comment by gmicha@gmail.com on 5 Jul 2010 at 8:56

• Changed state: Duplicate