Hi there, when running the readthrough mode I keep getting this error.
(artdeco) [mfaleeva@papr-res-compute07 ARTDeco-master_3]$ ARTDeco -mode readthrough -gtf-file /home/mfaleeva/ARTDeco-master_3/newgenes.gtf [-home-dir /home/mfaleeva/ARTDeco-master_3 -bam-files-dir /home/mfaleeva/ARTDeco-master_3/bam -layout SE -stranded TRUE -orientation Reverse -cpu 8 ]
Running readthrough mode...
Loading ARTDeco file structure...
ARTDeco will generate the following files:
./quantification/readthrough.raw.txt
./quantification/max_isoform.txt
./readthrough/readthrough.txt
./quantification/gene.exp.raw.txt
./readthrough/read_in_assignments.txt
./readthrough/corrected_exp.txt
./quantification/gene.exp.fpkm.txt
./readthrough
./readthrough/read_in.txt
./quantification/read_in.raw.txt
GTF file needed... Checking...
GTF file exists...
BAM file format needed... Checking... Will infer if not user-specified.
BAM files specified as single-end...
BAM files specified as stranded...
BAM files specified as reverse-strand oriented...
Summarizing BAM file stats...
8 Experiments
Files are Single-End, Strand-Specific, Reverse-strand oriented
Experiment Total Reads Mapped Reads
/home/mfaleeva/ARTDeco-master_3/bam/x5NP_LATE_R1_S2.sort.dedup.bam 20052400 20052400
/home/mfaleeva/ARTDeco-master_3/bam/x5dP_LATE_R1_S4.sort.dedup.bam 18114441 18114441
/home/mfaleeva/ARTDeco-master_3/bam/x5NP_LATE_R2_S10.sort.dedup.bam 1973197 1973197
/home/mfaleeva/ARTDeco-master_3/bam/x5dP_LATE_R2_S12.sort.dedup.bam 30559820 30559820
/home/mfaleeva/ARTDeco-master_3/bam/x5dD_LATE_R2_S11.sort.dedup.bam 20323589 20323589
/home/mfaleeva/ARTDeco-master_3/bam/x5ND_LATE_R1_S1.sort.dedup.bam 15258988 15258988
/home/mfaleeva/ARTDeco-master_3/bam/x5ND_LATE_R2_S9.sort.dedup.bam 22254209 22254209
/home/mfaleeva/ARTDeco-master_3/bam/x5dD_LATE_R1_S3.sort.dedup.bam 20609729 20609729
Generating gene expression files...
Traceback (most recent call last):
File "/home/mfaleeva/.conda/envs/artdeco/bin/ARTDeco", line 33, in <module>
sys.exit(load_entry_point('ARTDeco==0.4', 'console_scripts', 'ARTDeco')())
File "/home/mfaleeva/.conda/envs/artdeco/lib/python3.10/site-packages/ARTDeco-0.4-py3.10.egg/ARTDeco/main.py", line 472, in main
File "/home/mfaleeva/.conda/envs/artdeco/lib/python3.10/site-packages/ARTDeco-0.4-py3.10.egg/ARTDeco/readthrough.py", line 59, in get_multi_gene_exp
File "/home/mfaleeva/.conda/envs/artdeco/lib/python3.10/site-packages/ARTDeco-0.4-py3.10.egg/ARTDeco/readthrough.py", line 59, in <lambda>
File "/home/mfaleeva/.conda/envs/artdeco/lib/python3.10/site-packages/pandas/core/reshape/merge.py", line 110, in merge
op = _MergeOperation(
File "/home/mfaleeva/.conda/envs/artdeco/lib/python3.10/site-packages/pandas/core/reshape/merge.py", line 707, in __init__
self._maybe_coerce_merge_keys()
File "/home/mfaleeva/.conda/envs/artdeco/lib/python3.10/site-packages/pandas/core/reshape/merge.py", line 1340, in _maybe_coerce_merge_keys
raise ValueError(msg)
ValueError: You are trying to merge on float64 and object columns. If you wish to proceed you should use pd.concat
All the preprocessing files have been generated without any problem. Any suggestions? (My apologies for the frequent queries, its my first time working in conda, I really appreciate your help).
Hi there, when running the readthrough mode I keep getting this error.
All the preprocessing files have been generated without any problem. Any suggestions? (My apologies for the frequent queries, its my first time working in conda, I really appreciate your help).