Sorry again for ask this question here because I can not find a issue page or your email in the stringtie2 page. You can close this issue whenever you like.
From your paper about stringtie2, I believe i can handle my Ont RNA reads well with it.
But I get a much larger transcripts(about 30-40k) from stringtie2 no matter use ONT reads or Illumina reads., but the actually length should be 3-4k. I did all these with default paras and the input bam all result from hisat and minimap2.
Could you please give some tips about this or How should I process my Ont reads for genome annotation.
Thanks a lot.
Hello, Dr Skovaka:
Sorry again for ask this question here because I can not find a issue page or your email in the stringtie2 page. You can close this issue whenever you like. From your paper about stringtie2, I believe i can handle my Ont RNA reads well with it. But I get a much larger transcripts(about 30-40k) from stringtie2 no matter use ONT reads or Illumina reads., but the actually length should be 3-4k. I did all these with default paras and the input bam all result from hisat and minimap2. Could you please give some tips about this or How should I process my Ont reads for genome annotation. Thanks a lot.
ZhangZhou from JXAU,Nanchang,China.