question about using dorado

skovaka / uncalled4

MIT License

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question about using dorado #15

Closed mocherry closed 5 months ago

mocherry commented 5 months ago

Hi,

I would like to ask a question about using Dorado for basecalling sequencing data obrained with the RNA004 kit: when looking at dorado basecaller -h I cannot find the --moves_out option. Howver you say: --bam-in must be a BAM file produced by Guppy or Dorado using the --moves_out option. Is this option something Guppy has and Dorado has not?

Thanks and bes Matthias

skovaka commented 5 months ago

Sorry about that, for Dorado the flags are --emit-moves --emit-sam. I just updated the README with that info