Undetermined issue with peak detection?

smith-chem-wisc / FlashLFQ

Ultra-fast label-free quantification algorithm for mass-spectrometry proteomics

GNU Lesser General Public License v3.0

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Undetermined issue with peak detection? #77

Closed Dmorgen closed 5 years ago

Dmorgen commented 5 years ago

Hi guys,

So I've ran the software against some data I've got from Byonic. despite seeing good peaks in the raw files, I get "Identified and not quantified" or "Not detected" in the output. The only thing I'm not sure about is the monoisotopic mass - is it the deconvoluted one? if not should I add to the ID table the same sequence with other isotopes? please let me know if you'd like any files.

Thanks! D.

rmillikin commented 5 years ago

The monoisotopic mass should be the theoretical mass - is that what you mean?

If you can share the files with us, that would be a good way to solve this I think. Can you email me a link to spectra/PSM files?