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smith-chem-wisc / FlashLFQ

Ultra-fast label-free quantification algorithm for mass-spectrometry proteomics
GNU Lesser General Public License v3.0
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Crash with header / PSM identification issues #78

Closed Dmorgen closed 5 years ago

Dmorgen commented 5 years ago

Hi Guys,

got the following error: Data files were processed on a VMware, Inc. VMware Virtual Platform computer using Windows version 6.1.7601.65536 with a 64-Bit processor and 26 cores operating at 2.2GHz and 128GB installed RAM. .NET Framework Version: 4.7 or laterThermo MSFileReader Fileio DLL Version: 3.0 Thermo MSFileReader Fregistry DLL Version: 3.0 Thermo MSFileReader Rawfile Com Interface Version: 3.0.31.0

e: System.Exception: Could not interpret PSM header labels from file: D:\fass\Qsox1 ox. FlashLFQ data.txt at CMD.PsmReader.ReadPsms(String filepath, Boolean silent, List1 rawfiles) at GUI.MainWindow.RunFlashLfq() in C:\Users\rmillikin\Source\Repos\FlashLFQ_Master\GUI\MainWindow.xaml.cs:line 305e.Message: Could not interpret PSM header labels from file: D:\fass\Qsox1 ox. FlashLFQ data.txte.InnerException: e.Source: CMDe.StackTrace: at CMD.PsmReader.ReadPsms(String filepath, Boolean silent, List1 rawfiles) at GUI.MainWindow.RunFlashLfq() in C:\Users\rmillikin\Source\Repos\FlashLFQ_Master\GUI\MainWindow.xaml.cs:line 305e.TargetSite: System.Collections.Generic.List1[FlashLFQ.Identification] ReadPsms(System.String, Boolean, System.Collections.Generic.List1[FlashLFQ.SpectraFileInfo])

attached is the ID file Qsox1 ox. FlashLFQ data.txt

thanks! D.

trishorts commented 5 years ago

Hi D. I'm sure Rob will look at this as soon as he gets in. Are you going to ASMS? Let us know is you have posters/presentations so we can stop by.

rmillikin commented 5 years ago

Hi David,

The headers in the ID file are case-sensitive. The "peptide monoisotopic mass" label must instead be "Peptide Monoisotopic Mass".

Hope to see you at ASMS!

Dmorgen commented 5 years ago

Sorry about that – I’ve missed it… I won’t be coming to ASMS this year. I’m going to HUPO instead. However, I should visit the Coon lab soon, so I might drop by…

Thanks!

From: Robert Millikin [mailto:notifications@github.com] Sent: Wednesday, May 22, 2019 5:17 PM To: smith-chem-wisc/FlashLFQ FlashLFQ@noreply.github.com Cc: David Morgenstern david.morgenstern@weizmann.ac.il; Author author@noreply.github.com Subject: Re: [smith-chem-wisc/FlashLFQ] Crash with header / PSM identification issues (#78)

Hi David,

The headers in the ID file are case-sensitive. The "peptide monoisotopic mass" label must instead be "Peptide Monoisotopic Mass".

Hope to see you at ASMS!

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