Ribotricer Output

[bshim181]

Hello, This is my first time using ribotricer to interpret the ribosome sequencing dataset. I was kind of confused by what the proportion meant in the metagene profile graph. is the proportion of reads that are 29nt and 30nt long / total number of reads that in the bam file that have aligned to the translating ORFs?

Also, I was only able to find metagene profile for reads of these two lengths (29 nt and 30 nt). In the RibORF characterization,

I was able to notice level of periodicity in reads of other lengths as well. I was wondering what might have happened to reads of those lengths.

This is the bam file summary.

[saketkc]

We should clarify the meaning of the percentage in the documentation. But to clarify, ribotricer only plots metagene profile of read lengths that pass the phase score cutoff. In this case you just have two read lengths (29,30) that meet this criterion. Hope this helps. Feel free to reopen if there are any follow up questions.