Can ribotricer infer translation start position?

smithlabcode / ribotricer

A tool for accurately detecting actively translating ORFs from Ribo-seq data

http://doi.org/djv4

GNU General Public License v3.0

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Can ribotricer infer translation start position? #86

Closed freeedog closed 2 years ago

freeedog commented 2 years ago

Continued from #45

Hi,

I mean that there is some hint of where tranlation initiated even in normal Riob-seq without special inhibitor. For example, well can tell where the translation started in the following situation, for there is no reads or no periodic reads before the second start codon:

saketkc commented 2 years ago

Sorry to have missed your original question. Without a translation initiation inhibitor (such as harringtonine or lactimidomycin), it is not possible to predict the initiation site.