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smorabit / hdWGCNA

High dimensional weighted gene co-expression network analysis
https://smorabit.github.io/hdWGCNA/
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Question about [SetDatExpr() error] #188

Closed lisp-0326 closed 5 months ago

lisp-0326 commented 5 months ago

SetDatExpr() error Hi, When I run hdWGNA, using the sample data（Zhou_2020.rds）, I always get an error when reaching this SetDatExpr() function. Error in GetAssayData(): ! GetAssayData doesn't work for multiple layers in v5 assay.

I want to know how to solve

seurat_obj <- SetDatExpr(

  seurat_obj,

  group_name = "INH", # the name of the group of interest in the group.by column

  group.by='cell_type', # the metadata column containing the cell type info. This same column should have also been used in MetacellsByGroups

  assay = 'RNA', # using RNA assay

  slot = 'data' # using normalized data )

venkan commented 5 months ago

Hello,

Looks like you working with Seurat v5. Better use Seurat v4.3.0 you won't be having this error.