Closed splaisan closed 6 years ago
I deleted the two results folders and re-ran the full script but the bam's do not get indexed What I am missing? Thanks
rule bwa_map: input: "data/genome.fa", "data/samples/{sample}.fastq" output: "mapped_reads/{sample}.bam" shell: "bwa mem {input} | samtools view -Sb - > {output}" rule samtools_sort: input: "mapped_reads/{sample}.bam" output: "sorted_reads/{sample}.bam" shell: "samtools sort -T sorted_reads/{wildcards.sample} " "-O bam {input} > {output}" rule samtools_index: input: "sorted_reads/{sample}.bam" output: "sorted_reads/{sample}.bam.bai" shell: "samtools index {input}" > snakemake sorted_reads/{A,B}.bam Building DAG of jobs... Using shell: /bin/bash Provided cores: 1 Rules claiming more threads will be scaled down. Job counts: count jobs 2 samtools_sort 2 [Mon Oct 22 11:40:17 2018] rule samtools_sort: input: mapped_reads/B.bam output: sorted_reads/B.bam jobid: 0 wildcards: sample=B [Mon Oct 22 11:40:17 2018] Finished job 0. 1 of 2 steps (50%) done [Mon Oct 22 11:40:17 2018] rule samtools_sort: input: mapped_reads/A.bam output: sorted_reads/A.bam jobid: 1 wildcards: sample=A [Mon Oct 22 11:40:17 2018] Finished job 1. 2 of 2 steps (100%) done Complete log: /data/NC_projects/snakemake-tutorial/.snakemake/log/2018-10-22T114017.262317.snakemake.log # is leading to > ls -lah sorted_reads/ total 4.4M drwxr-xr-x 2 u0002316 domain users 4.0K Oct 22 11:30 . drwxr-xr-x 6 u0002316 domain users 4.0K Oct 22 11:39 .. -rw-r--r-- 1 u0002316 domain users 2.2M Oct 22 11:30 A.bam -rw-r--r-- 1 u0002316 domain users 2.2M Oct 22 11:30 B.bam
Duplicate of #10.
I deleted the two results folders and re-ran the full script but the bam's do not get indexed What I am missing?
Thanks