Open cbonnefoy opened 4 years ago
The p-value comes from the regression done by Hmisc::rcorr
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I am not a statistician, so I won't explain but the documentation says "[...] P, the asymptotic P-values".
I think you can find an explanation here: https://statisticsbyjim.com/regression/interpret-coefficients-p-values-regression/
It is unclear if you question was something else...
Thanks for your comment Jan.
Now I can calculate the p_values by Hmisc::rcorr.
I understand it is to test the significativity of the correlation which depends on the number of pairs used. Because I have only 12 samples, even a correlation of 0.6 pass the test (= is less than 0.05).
What I don't understand is why two variables that are in the same pseudospectra before groupCorr are splitted in two different after, knowing that the correlation is >0.90 and the significativity far less than 0.05
Sounds strange. Are they the only features in the group?
you could try graphMethod="lpc"
to see if it is the clustering algorithm that does something strange.
I think example data is probably needed to investigate this further.
Yes when they split they are often the only feature in the group
I only test graphMethod for one psg_list. They both result in splitting, even if the splitting is sligthly different.
I noticed that the number of the pcgroups after splitting are very near, they only differ by one. It seems to me that the features are rejected from the originating group but I don't know how
Here is an example for 3 molecules. Have a look at carbamazepine
Thanks
FWHM_Corr_1_12_Carbamazepine_Sulfamethoxazole_Ketoprofene.xlsx
Which intensity values did you use for that analysis? You used maxo for groupFWHM but default for groupCorr is into. I am wondering if there are some NA values in play. Did you fill peaks?
I used maxo for groupCorr too. I filled peaks but for some features there are still many NA.
I agree a part of my problem can come from that but I think there is another reason because I have examples where no peak is missing.
Hello and thanks in advance for you help
I am using CAMERA
xsgf<-as(fdata,"xcmsSet") xsaf<-xsAnnotate(xsgf,sample = c(3:5), polarity = "positive") xsaFf<-groupFWHM(xsaf, sigma = 6 , perfwhm = 0.6, intval = "maxo") xsaFCIf<-findIsotopes(xsaFf, maxcharge=3, maxiso=4, ppm=5, mzabs=0.015, intval="maxo", minfrac=1, isotopeMatrix = NULL,filter = TRUE) xsFCf <- groupCorr(xsaFCIf, cor_exp_th = 0.90,calcCiS = FALSE, calcCaS = TRUE) xsaFCIAf<-findAdducts(xsFCf,polarity = "positive")
At the end, I get groups in different ps-groups
Example
line 2227 and line 2239 after FWHM pc-group = 21 for both after isotopes, corr and adducts pc-group = 756 and 757 respectively the correlation coefficient calculated by calcCaS(xsaFCIf,corval=0.90, pval=0.05, intval="maxo") is 0.9999
Could someone explain me why? What is the role of the pval? How could I access it?
Christelle