Per read it is known which protease generated the peptide, this can be used in the assignment of hard amino acids. Chymotrypsin is known to have specificity after I/L. But a simple search with a sample dataset using database matching gave the impression that this specificity is not 100%. TO actually implement this a more accurate idea on the specificity is needed, after that these specificaties can be weighed in into the consensus sequence.
Per read it is known which protease generated the peptide, this can be used in the assignment of hard amino acids. Chymotrypsin is known to have specificity after I/L. But a simple search with a sample dataset using database matching gave the impression that this specificity is not 100%. TO actually implement this a more accurate idea on the specificity is needed, after that these specificaties can be weighed in into the consensus sequence.