Open xiaonalu opened 1 year ago
Sounds like a duplicate to #42
We knew the cas-offinder output. But what can we next do to generate "off-target score" for evaluating off-target efficiency. In other words, we need a value to judge a gRNA good or bad relatively.
I see, I have a plan to upgrade the web interface soon, but it had to be delayed due to the shortage of R&D funding in Korea. We hope to finish this within this year. The progress will be trackable at: https://github.com/pnucolab
I see, I have a plan to upgrade the web interface soon, but it had to be delayed due to the shortage of R&D funding in Korea. We hope to finish this within this year. The progress will be trackable at: https://github.com/pnucolab
Thanks for your soon reply! And recently i'm designing gRNAs in batch based on NGG rules. Then i wanna evaluate the off-target efficiency of these gRNAs by cas-offinder for further filter. I started to know cas-offinder from a pubished paper in Nature Genetics. They said they identified off-target scores by Cas-OFFinder. I have no idea about the raw Cas-OFFinder output. Do you have any suggestion for me?
I think i see how to use the raw Cas-OFFinder output. Just count the match records for each gRNA. Only one record is the best design!
Could the outcome be integrated with statistics, to guide further wet experiments to detect high risk off target event? Thanks