Open danli349 opened 5 months ago
When I run the tutorial https://snystrom.github.io/memes-manual/articles/integrative_analysis.html I found this error again:
head(by_binding)
BStringSetList of length 3
[["ectopic"]] chr3L:40482-40581=GGACTGGGGTTGGGTACGAAACGAAATGGAAACATAGCCAAACAC...
[["entopic"]] chr3L:60616-60715=CAGGTGCATCCCGCGACGAGTATTTTTTATATTATTGTTTGGCCG...
[["orphan"]] chr3L:57749-57848=GAAACCTATACCCCGCCGTCGGCCGATTATCACCTTTTCAAGTCTC...
ame_by_binding <- by_binding %>%
runAme
Added /tmp/RtmpD0mwb1/file62f8791c7d96.meme to motif_sources which now has 1 file names. Motif file name is /tmp/RtmpD0mwb1/file62f8791c7d96.meme. Writing results to output directory '/tmp/RtmpD0mwb1/file62f8206069aa_vs_shuffle'. E-value threshold for reporting results: 10 Checking alphabets in 1 motif files. Loading motifs from file '/tmp/RtmpD0mwb1/file62f8791c7d96.meme' Loading primary sequences. Creating control sequences by shuffling input sequences preserving 2-mers. Not in partition maximization mode. Fixing partition at the number of primary sequences (2421). Bad file name. Bad file name. Bad file name. Bad file name. Bad file name. Bad file name. Bad file name. Bad file name. Bad file name. Bad file name. Bad file name. Bad file name. FATAL: Template does not contain data section.
Error in purrr::map()
:
ℹ In index: 1.
ℹ With name: ectopic.
Caused by error:
! Shell process had non-zero exit status.
Traceback:
Hi Dan,
Sorry for just now seeing this issue, I was sick the week you reported & this slipped through the cracks!
Would you mind sharing how you installed the meme suite? I believe this error can happen when AME is missing a core file in its install. Perhaps rerun your runAme
call using verbose = TRUE
and post the resulting log.
I see you are running MEME Suite 5.5.5 which I have not tested myself, so perhaps the developers made a change. I can also take a look at their release notes for any clues.
Another way to test is to attempt to run ame
at the commandline and see if it still errors. That may help rule out whether the R code or the install itself is at fault.
Sorry again for the delay.
Hello:
I run the example codes in the
README
:How should I fix this? Thanks