soedinglab / MMseqs2

MMseqs2: ultra fast and sensitive search and clustering suite
https://mmseqs.com
GNU General Public License v3.0
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tmp/3407079133035171875/tmp_hsp1/941510883324284880/blastp.sh: line 99: 13762 Killed #697

Open zzsunday opened 1 year ago

zzsunday commented 1 year ago

Expected Behavior

assign taxonomy to Metagenome contigs, and the database is created from krakenuniq bacterial database.

Current Behavior

tmp/3407079133035171875/tmp_hsp1/941510883324284880/blastp.sh: line 99: 13762 Killed $RUNNER "$MMSEQS" prefilter "$INPUT" "$TARGET" "$TMPPATH/pref$STEP" $PREFILTER_PAR -s "$SENS" Error: Prefilter died Error: Search step died Error: First search died

Steps to Reproduce (for bugs)

Please make sure to execute the reproduction steps with newly recreated and empty tmp folders. mmseqs createdb ~/04.db/krakenuniq/library/nt-bacteria-dustmasked.fna nt-bacteria-dustmasked --dbtype 2 mmseqs createtaxdb nt-bacteria-dustmasked tmp --ncbi-tax-dump ~/04.db/krakenuniq/taxonomy --tax-mapping-file ~/04.db/krakenuniq/library/nt-bacteria.fna.map mmseqs createdb BIN_REASSEMBLY/reassembled_bins/bin.18.strict.fa test mmseqs taxonomy --threads 16 test ~/04.db/mmseqs/taxonomy/nt-bacteria-dustmasked test_res tmp --search-type 3

MMseqs Output (for bugs)

Please make sure to also post the complete output of MMseqs. You can use gist.github.com for large output. Header split mode 0 Chain overlapping alignments 0 Merge query 1 Search type 3 Exhaustive search mode false Filter results during exhaustive search 0 Strand selection 1 LCA search mode false Disk space limit 0 MPI runner Force restart with latest tmp false Remove temporary files false

Accel. 2bLCA cannot be used with nucl-nucl taxonomy, using top-hit instead search test /public/home/bh2022044/04.db/mmseqs/taxonomy/nt-bacteria-dustmasked tmp/3407079133035171875/first tmp/3407079133035171875/tmp_hsp1 --alignment-mode 1 -e 1 --max-rejected 5 --max-accept 30 --threads 16 -s 2 --mask 0 --spaced-kmer-mode 1 --min-length 30 --max-length 32734 --orf-start-mode 1 --search-type 3

prefilter tmp/3407079133035171875/tmp_hsp1/941510883324284880/query_seqs_split tmp/3407079133035171875/tmp_hsp1/941510883324284880/target_seqs_split tmp/3407079133035171875/tmp_hsp1/941510883324284880/search/pref_0 --sub-mat 'aa:blosum62.out,nucl:nucleotide.out' --seed-sub-mat 'aa:VTML80.out,nucl:nucleotide.out' -k 15 --k-score seq:2147483647,prof:2147483647 --alph-size aa:21,nucl:5 --max-seq-len 10000 --max-seqs 300 --split 0 --split-mode 2 --split-memory-limit 0 -c 0 --cov-mode 0 --comp-bias-corr 1 --comp-bias-corr-scale 1 --diag-score 1 --exact-kmer-matching 1 --mask 0 --mask-prob 0.9 --mask-lower-case 0 --min-ungapped-score 15 --add-self-matches 0 --spaced-kmer-mode 1 --db-load-mode 0 --pca substitution:1.100,context:1.400 --pcb substitution:4.100,context:5.800 --threads 16 --compressed 0 -v 3 -s 2.0

Query database size: 960 type: Nucleotide Estimated memory consumption: 1T Target database size: 26422067 type: Nucleotide Index table k-mer threshold: 0 at k-mer size 15 Index table: counting k-mers [> ] 0.00% 1 eta -

tmp/3407079133035171875/tmp_hsp1/941510883324284880/blastp.sh: line 99: 13762 Killed $RUNNER "$MMSEQS" prefilter "$INPUT" "$TARGET" "$TMPPATH/pref$STEP" $PREFILTER_PAR -s "$SENS" Error: Prefilter died Error: Search step died Error: First search died

Context

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Your Environment

Include as many relevant details about the environment you experienced the bug in.

system:linux Architecture: x86_64 CPU op-mode(s): 32-bit, 64-bit Byte Order: Little Endian CPU(s): 56 On-line CPU(s) list: 0-55 Thread(s) per core: 1 Core(s) per socket: 28 Socket(s): 2 NUMA node(s): 2 Vendor ID: GenuineIntel CPU family: 6 Model: 106 Model name: Intel(R) Xeon(R) Gold 6348 CPU @ 2.60GHz

abdo3a commented 10 months ago

Hi @zzsunday, i'm facing the same error. did you manage to figure out the solution?