Error running mpi job with class

[liuxianghui]

Dear sir: I have no problem running a single plass job on my Linux cluster. However, I want to try for mpi plass job. Here are the details for the input and logs files. I specify two nodes with each 32 cores with PBS -l nodes=2:ppn=32. In the job, I specify mpirun -np 64. I ran into error with writting to /tmp file. The problem also happens if I specify a local tmp file folder. Please kindly suggest,

The sh file is like this:

!/bin/bash

PBS -N mpi

name you want to give your job

the default output file will use this

PBS -q std

specify the queue you want to use

PBS -l nodes=2:ppn=32

PBS -j oe

PBS -o logs

=======================================================

LOAD PBS MODULES

=======================================================

cd $PBS_O_WORKDIR

module load openmpi3/gcc/64/3.1.4 module load pbs

cd $PBS_O_WORKDIR

export OMP_NUM_THREADS=1

mpirun -np 42 plass assemble examples/reads_1.fastq.gz examples/reads_2.fastq.gz assembly.fas tmp

plass assemble examples/reads_1.fastq.gz examples/reads_2.fastq.gz assembly.fas tmp

for bin in $(cat list2) do echo ${bin} mpirun -np 64 plass assemble $bin'_tr_hostout_R1.fastq' $bin'_tr_hostout_R2.fastq' $bin'.assembly.plass.protein.fas' /tmp

plass nuclassemble $bin'_tr_hostout_R1.fastq' $bin'_tr_hostout_R2.fastq' $bin'.assembly.plass.nuc.fas' tmp

echo "###Assembly Sample" $bin" Start###" date done

list2 file: P016_S9

The logs file is like the below:

P016_S9 assemble P016_S9_tr_hostout_R1.fastq P016_S9_tr_hostout_R2.fastq P016_S9.assembly.plass.protein.fas /tmp

assemble P016_S9_tr_hostout_R1.fastq P016_S9_tr_hostout_R2.fastq P016_S9.assembly.plass.protein.fas /tmp

assemble P016_S9_tr_hostout_R1.fastq P016_S9_tr_hostout_R2.fastq P016_S9.assembly.plass.protein.fas /tmp

assemble P016_S9_tr_hostout_R1.fastq P016_S9_tr_hostout_R2.fastq P016_S9.assembly.plass.protein.fas /tmp

assemble P016_S9_tr_hostout_R1.fastq P016_S9_tr_hostout_R2.fastq P016_S9.assembly.plass.protein.fas /tmp

assemble P016_S9_tr_hostout_R1.fastq P016_S9_tr_hostout_R2.fastq P016_S9.assembly.plass.protein.fas /tmp

MMseqs Version: 3.764a3 Substitution matrix nucl:nucleotide.out,aa:blosum62.out Rescore mode 3 Allow wrapped scoring false Remove hits by seq. id. and coverage false E-value threshold 1e-05 Coverage threshold 0 Add backtrace MMseqs Version: 3.764a3 Substitution matrix nucl:nucleotide.out,aa:blosum62.out Rescore mode 3 Allow wrapped scoring false Remove hits by seq. id. and coverage false E-value threshold 1e-05 Coverage threshold 0 Add backtrace false Coverage mode 0 Seq. id. threshold 0.9 Min. alignment length 0 Seq. id. mode 0 Include identical seq. id. false Sort results 0 Preload mode 0 Threads 64 Compressed 0 Verbosity 3 Alphabet size 13 K-mers per sequence 60 scale k-mers per sequence 0 Adjust k-mer length false Mask residues 0 Mask lower case residues 0 K-mer size 14 Max sequence length 65535 Shift hash 5 Split memory limit 0 Include only extendable true Skip repeating k-mers true Min codons in orf 45 Max codons in length 32734 Max orf gaps 2147483647 Could not delete /tmp/latest! Could not create symlink of /tmp/531455983002076514! Could not delete /tmp/latest! Could not delete /tmp/latest! Could not delete /tmp/latest! Could not write file /tmp/531455983002076514/assembler.sh! Could not delete /tmp/latest! Could not delete /tmp/latest! Could not delete /tmp/latest! Could not write file /tmp/531455983002076514/assembler.sh! Could not delete /tmp/latest! Could not delete /tmp/latest! Could not delete /tmp/latest! Could not delete /tmp/latest! Could not delete /tmp/latest!

Expected Behavior

Current Behavior

Steps to Reproduce (for bugs)

Please make sure to execute the reproduction steps with newly recreated and empty tmp folders.

Plass Output (for bugs)

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Context

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Your Environment

Include as many relevant details about the environment you experienced the bug in.

• Git commit used (The string after "Plass Version:" when you execute Plass without any parameters):
• Which Plass version was used (Statically-compiled, self-compiled, Homebrew, etc.):
• For self-compiled and Homebrew: Compiler and Cmake versions used and their invocation:
• Server specifications (especially CPU support for AVX2/SSE and amount of system memory):
• Operating system and version:

[martin-steinegger]

Thank you for reporting this issues. The MPI is currently broken in PLASS, please use the single version in the mean time.