timestamps

[joaomamede]

Hi,

Thank you for pims_nd2.

IS there anyway of retrieving the time stamps metadata?

​

>frames =  pims.ND2_Reader('E:/JM/igfp3_caruby3_rutin_BVD_F2_2days.nd2')

>frames.bundle_axes = 'zyx'  # when 'z' is available, this will be default

>​print(frames.sizes)

>>    {'x': 2048, 'y': 2044, 'c': 3, 't': 126, 'm': 23, 'z': 21}

> meta = frames.metadata

> print(meta)

 >> {'width': 2048, 'width_bytes': 12288, 'height': 2044, 'components': 3, 'bitsize_memory': 16, 'bitsize_significant': 16, 'sequence_count': 60858, 'tile_width': 2048, 'tile_height': 2044, 'compression': None, 'compression_quality': 4294967197, 'plane_count': 3, 'angle': -1.5867835237991448, 'calibration_um': 0.108333333333333, 'time_start_jdn': 2459020.447350243, 'time_start': datetime.datetime(2020, 6, 19, 15, 44, 11, 61004), 'time_start_utc': datetime.datetime(2020, 6, 19, 22, 44, 11, 61004), 'objective': 'Plan Apo λ 60x Oil', 'magnification': -1.0, 'NA': 1.4, 'refractive_index1': 1.515, 'refractive_index2': 1.515, 'pinhole': 0.0, 'zoom': 1.0, 'projective_mag': -1.0, 'image_type': 'normal', 'z_home': 0, 'plane_0': {'components': 1, 'rgb_value': (0.0, 0.0, 1.0), 'name': 'a647', 'oc': 'a647', 'emission_nm': 670.0}, 'plane_1': {'components': 1, 'rgb_value': (1.0, 0.0, 0.0), 'name': 'mRuby', 'oc': '', 'emission_nm': 620.0}, 'plane_2': {'components': 1, 'rgb_value': (0.0, 1.0, 0.0), 'name': 'GFP', 'oc': '', 'emission_nm': 535.0}}

This is what I get from a 176 time frame file. I have timelapses that range from 5 minutes, to 15minutes and then I finish with 30mins over 2days, so I need the exact deltaT between frames.

Best