Open ManuelMoradiellos opened 1 year ago
Hey Manuel, I had the same issue as you when performing some comparisons between groups. First comparisons went smooth. But on the later ones I got the same error as you.
The problem was that not all the conditions shared cells on all clusters. Therefore if you have any cluster/cell group that is not present in one of the conditions the analysis won't run.
How to solve it. Just subset the your object on both conditions to make sure that you have the same clusters/cell groups and re-run the cellchat analysis.
Hi all!
I've used CellChat the previous month for an important experiment that needed some revisiting now. While looking at the update I wanted to use some of the new functions available to obtain a general overview of the pathways being "communicated" within my experiment.
In my experiment I have three different conditions that I store in CellChat objects like so (from scratch. not updating previous objects):
Next I follow the new part of the vignette (specifically Part IV) but whenever I want to use any of the functions on one of my experimental conditions-specific object I get an error:
I don't know exactly what I'm doing wrong and when I checked for past issues some looked pretty similar (#479 ) but their solution didn't help me or it did not seem to fit what I want to do, which is working on separate objects per experimental condition to check overall trends to later focus on.
Thanks in advance and sorry if this is a stupid question