How to get liquid Nitrogen/Hobo logging

[grace-ac]

I'm going to Taylor Shellfish Hatchery tomorrow to meet Rhonda @Ellior2 . Was asked to bring liquid nitrogen. Does anyone know how to get it?

Or, @Ellior2, would a cooler with dry ice work?

[Ellior2]

Well the reason I like liquid nitrogen is because it will last for a whole month of sampling. I don't think dry ice will last longer than a day or two. I have gone to Airgas in Bremerton in the past with our dewar or you could get it from the UW. @kubu4 or @sr320 could give you tips on how to get it.

Also important- I would like to hook up these hobos you gave me. Do you guys have a logger and all the necessary equipment/manuals to get them going? I have never used them before...

[seanb80]

We have everything to set them up. How can be found at

https://seanb80.github.io/blog/2016/12/20/Prepping-HOBO-loggers

[sr320]

UW Chem Dept provides LN, but you have to be trained to dispense. Maybe Grace could go there in morning?

You have a -80? Correct?

[Ellior2]

I just checked in with the guys at Bremerton Airgas. They have about 80L, plenty for our needs.. They open at 8AM. You could take the Seattle to Bremerton Ferry and stop by Airgas on the way to the hatchery. This is an option, or you can try to figure out the UW situation...

We do have a -80C. I haven't gone down there in a while to look at it but will do so tomorrow.

[sr320]

Bremerton Airgas would probably be simpler and faster as stock room might not even open until 9...

On Thu, May 4, 2017 at 5:05 PM Rhonda Elliott notifications@github.com wrote:

I just checked in with the guys at Bremerton Airgas. They have about 80L, plenty for our needs.. They open at 8AM. You could take the Seattle to Bremerton Ferry and stop by Airgas on the way to the hatchery. This is an option, or you can try to figure out the UW situation...

We do have a -80C. I haven't gone down there in a while to look at it but will do so tomorrow.

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[Ellior2]

I also briefly looked at the hobos today and they look much different than the ones @seanb80 writes about. The ones I got measure temperature AND light... Is the same equipment used to set them up?

[seanb80]

I'm not sure, but I can look in to it!

On May 4, 2017, at 5:20 PM, Rhonda Elliott notifications@github.com wrote:

I also briefly looked at the hobos today and they look much different than the ones @seanb80 writes about. The ones I got measure temperature AND light... Is the same equipment used to set them up?

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[sr320]

We really only have one kind. On Thu, May 4, 2017 at 5:28 PM seanb80 notifications@github.com wrote:

I'm not sure, but I can look in to it!

On May 4, 2017, at 5:20 PM, Rhonda Elliott notifications@github.com wrote:

I also briefly looked at the hobos today and they look much different than the ones @seanb80 writes about. The ones I got measure temperature AND light... Is the same equipment used to set them up?

— You are receiving this because you were mentioned. Reply to this email directly, view it on GitHub, or mute the thread.

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[hputnam]

http://www.onsetcomp.com/products/communications/base-u-4 The base station should come with all the couplers you need and list the number for the logger type on the side.

If you can't get the couplers but have the base station, you can just align the optics and hold or tape them there to read out the data. You have to align the dot and black square on the front of the logger with the matching set on the base station. http://www.onsetcomp.com/products/data-loggers/ua-002-08

I would highly recommend logging temp with all loggers in the same tank directly next to each other for at least 24h for cross validation prior to allocating them to separate tanks. Also as Sean says make sure to sync them all to start at the same time and log at the same interval, or your data will not be comparable.

[grace-ac]

Okay, so it sounds like I have to stop by FTR to pick up Dewar to use at Airgas. Anything else from FTR? On Thu, May 4, 2017 at 6:03 PM Hollie Putnam notifications@github.com wrote:

http://www.onsetcomp.com/products/communications/base-u-4 The base station should come with all the couplers you need and list the number for the logger type on the side.

If you can't get the couplers but have the base station, you can just align the optics and hold or tape them there to read out the data. You have to align the dot and black square on the front of the logger with the matching set on the base station. http://www.onsetcomp.com/products/data-loggers/ua-002-08

I would highly recommend logging temp with all loggers in the same tank directly next to each other for at least 24h for cross validation prior to allocating them to separate tanks. Also as Sean says make sure to sync them all to start at the same time and log at the same interval, or your data will not be comparable.

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[sr320]

Logger

On Fri, May 5, 2017 at 6:57 AM grace-ac notifications@github.com wrote:

Okay, so it sounds like I have to stop by FTR to pick up Dewar to use at Airgas. Anything else from FTR? On Thu, May 4, 2017 at 6:03 PM Hollie Putnam notifications@github.com wrote:

http://www.onsetcomp.com/products/communications/base-u-4 The base station should come with all the couplers you need and list the number for the logger type on the side.

If you can't get the couplers but have the base station, you can just align the optics and hold or tape them there to read out the data. You have to align the dot and black square on the front of the logger with the matching set on the base station. http://www.onsetcomp.com/products/data-loggers/ua-002-08

I would highly recommend logging temp with all loggers in the same tank directly next to each other for at least 24h for cross validation prior to allocating them to separate tanks. Also as Sean says make sure to sync them all to start at the same time and log at the same interval, or your data will not be comparable.

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.

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[sr320]

@Ellior2 Note your hobos should already be logging. Sean set them up. You can confirm with blink pattern (see manual sheet)

[hputnam]

blinking red light means logging. you can also check detailed status using hoboware pro to confirm logging settings

[grace-ac]

Well i just made a huge mistake- misheard the guy at the ferry. Bremerton ferry doesnt come until 10am!!! I just bought the ticket and everything :(((((( i should have just drove around.

On Fri, May 5, 2017 at 8:38 AM Hollie Putnam notifications@github.com wrote:

blinking red light means logging. you can also check detailed status using hoboware pro to confirm logging settings

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[grace-ac]

That's probably why my navigation didn't give me the ferry option! It takes longer than driving.

On Fri, May 5, 2017 at 8:52 AM Grace Crandall graceac9@uw.edu wrote:

Well i just made a huge mistake- misheard the guy at the ferry. Bremerton ferry doesnt come until 10am!!! I just bought the ticket and everything :(((((( i should have just drove around.

On Fri, May 5, 2017 at 8:38 AM Hollie Putnam notifications@github.com wrote:

blinking red light means logging. you can also check detailed status using hoboware pro to confirm logging settings

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[seanb80]

They have in the past let me get a refund if I decided not to take the ferry after queuing up, might be worth a shot!

[sr320]

Or you could just relax, grab a coffee, and catch some of lab meeting at https://hangouts.google.com/hangouts/_/6z4e4irui5a4dov6td22cmsacme :)

[Ellior2]

I see no sign of life on these hobos. No blinking lights. Manual says it will blink once every one to four seconds (the shorter the logging interval, the faster the light blinks), but then it says it blinks when it's logging a sample. What if it is set up to log once an hour? Am I missing the blink?

[seanb80]

Hm, I'm pretty sure I set them up, but it should be easy to check with the logger base once Grace brings it. I sent her with a serial number for the Hoboware software so you can download the software and use it where convenient.

[Ellior2]

So I downloaded the Hoboware for Windows but was not able to get the upgrade to Hoboware pro. When I clicked on the Hoboware Pro link, it brought me to a page where I could purchase it for $100 but I couldn't figure out how to enter in the product key. I registered and got an account and everything, tried putting it in my cart and looking for a place to enter the code but couldn't figure it out.

I tried to plug the shuttle in (without pendant) and use the program I downloaded: Hoboware for Windows. I don't see the "Sync Shuttle Clock" button that @seanb80 talks about and when I try to "select device" I get this...

[Ellior2]

Nevermind, I think I got it! I am going to put them all in one tank and check out the results Monday. Thanks all!