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Roberts Lab Documents
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Purchase supplies for protein extractions #611

Closed yaaminiv closed 7 years ago

yaaminiv commented 7 years ago

@laurahspencer and I looked through the lab today and we need the following:

Ellior2 commented 7 years ago

Really? I thought we got a TON of pH test strips when I started the extractions, in fact I don't even know if I broke into the new pack. Are the IAA and TCEP I made in the -80C used up or no good?

yaaminiv commented 7 years ago

@Ellior2 if there are extra pH strips, we definitely can't find them! There isn't a listing for pH strips on the lab inventory/location spreadsheet, last I checked.

The IAA and TCEP aren't all used up, but we're not sure if they're no good. @emmats would we be able to use IAA and TCEP made in November for our upcoming extractions? Either way, we may need more reagent because we're planning on doing more extractions in the future.

emmats commented 7 years ago

How were the IAA and TCEP stored? If both were frozen and IAA has been kept in the dark, then they should be fine.

Ellior2 commented 7 years ago

Just wanted to make sure you wouldn't be buying more when we still have a bunch. I also remember a little bit went a long way with those ones, so I am not sure if we still have the original bottle that would be good too. When I made the November batch of IAA for example, I only used 186mg to make 5ml and we bought 5g....

yaaminiv commented 7 years ago

In that case, here's our revised list:

Ellior2 commented 7 years ago

For pH test strips check out the upper counter behind that pH meter set up... in the office where Sam hangs out maybe 6 steps in on the right.

yaaminiv commented 7 years ago

We checked that spot and there are no more there!

sr320 commented 7 years ago

Budget: 61-0221

kubu4 commented 7 years ago

@yaaminiv and @laurahspencer - Please clarify the following:

laurahspencer commented 7 years ago

The Tris container in the chem locker is low- figured we needed more regardless (we use for OA projects too).

On Wed, May 10, 2017 at 10:03 AM kubu4 notifications@github.com wrote:

@yaaminiv https://github.com/yaaminiv and @laurahspencer https://github.com/laurahspencer - Please clarify the following:

  • need example of desired microplates (preferably with a Vendor and Catalog number)
  • Tris: We have this (i.e. >500g). Do you actually need more?

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kubu4 commented 7 years ago

There are two containers (I believe the other one is unopened).

kubu4 commented 7 years ago

Gah! Never mind! They are both open. Sorry!! I'll just order more.

yaaminiv commented 7 years ago

img_7355

Hopefully this will be the information you need for the microplates!

sr320 commented 7 years ago

Curious- what are these used for?

On Wed, May 10, 2017 at 2:09 PM Yaamini Venkataraman < notifications@github.com> wrote:

[image: img_7355] https://cloud.githubusercontent.com/assets/22335838/25921371/fbefe98c-3589-11e7-8985-c030a8529714.JPG

Hopefully this will be the information you need for the microplates!

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yaaminiv commented 7 years ago

@sr320 we use them in BCA assay to quantify the amount of protein in our sonicated samples. that number is then used to calculate the amount of a reagent we need to add for trypsin digestion

sr320 commented 7 years ago

Would something like this not be more appropriate? https://www.thermofisher.com/order/catalog/product/15045 On Wed, May 10, 2017 at 2:15 PM Yaamini Venkataraman < notifications@github.com> wrote:

@sr320 https://github.com/sr320 we use them in BCA assay to quantify the amount of protein in our sonicated samples. that number is then used to calculate the amount of a reagent we need to add for trypsin digestion

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yaaminiv commented 7 years ago

Probably! I just looked at what we used previously/what was already in the lab.

kubu4 commented 7 years ago

What type of 96-well plates does @emmats use for the BCA assay?

emmats commented 7 years ago

I'm not in the lab now, but will try to get you that info soon.

emmats commented 7 years ago

Brook ignored my request for this the other day, but here it is. Sorry for the delay. img_3243

kubu4 commented 7 years ago

Thanks.

laurahspencer commented 7 years ago

@kubu4 are there tracking numbers for any of these items? I'm particularly interested in the well plates. Thanks!

kubu4 commented 7 years ago

All items, with the exception of the pH strips have been received.

Plates were delivered today, so are likely still in the main office waiting for me to pick them up.

sr320 commented 7 years ago

Plates are not here- just confirmed with Laurie

kubu4 commented 7 years ago

Sorry. They get delivered tomorrow. Whoops!

kubu4 commented 7 years ago

@Ellior2 if there are extra pH strips, we definitely can't find them! There isn't a listing for pH strips on the lab inventory/location spreadsheet, last I checked.

For anyone who's still interested, I found the previously purchased pH strips that @Ellior2 referenced on the bench in front of the NanoPure in FTR 213, along with a few bottles of miscellaneous solutions (Solvent A, Solvent B, and some acetonitrile solution). I've left them all there, since it seems as though someone has placed them there intentionally.

laurahspencer commented 7 years ago

Yes, that was me who found everything and placed them there, as we will use them this week. The pH strips had been hiding under a towel.

On Mon, May 22, 2017 at 1:24 PM kubu4 notifications@github.com wrote:

@Ellior2 https://github.com/ellior2 if there are extra pH strips, we definitely can't find them! There isn't a listing for pH strips on the lab inventory/location spreadsheet, last I checked.

For anyone who's still interested, I found the previously purchased pH strips that @Ellior2 https://github.com/ellior2 referenced on the bench in front of the NanoPure in FTR 213, along with a few bottles of miscellaneous solutions (Solvent A, Solvent B, and some acetonitrile solution). I've left them all there, since it seems as though someone has placed them there intentionally.

— You are receiving this because you were mentioned. Reply to this email directly, view it on GitHub https://github.com/sr320/LabDocs/issues/611#issuecomment-303209991, or mute the thread https://github.com/notifications/unsubscribe-auth/AQdwfX8sH5E6-ilNrM6qydJ2jvjp0qc-ks5r8e7SgaJpZM4NUQNA .